Description

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies.

Key Features

@introbul:Key Features @bul:* Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions * Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT * Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence * Includes quality control information crucial in research and clinical laboratories * Most chapters are written by the inventors and principal developers of the methodologies * Includes color plates, 77 figures, and 18 tables

Readership

Researchers, graduate students, technicians, and clinicians in virology, microbiology, pathology, infectious disease, cytogenetics, molecular biology, and diagnostics.

Table of Contents

D.L. Wiedbrauk and A.M. Drevon, Nucleic Acid Detection Methods. D.L. Wiedbrauk and J. Stoerker, Quality Assurance in the Molecular Biology Laboratory. D.L. Stoler and N.L. Michael, Nucleic Acid Blotting Techniques for Virus Detection. J.Carr, In Situ Hybridization. R.L. Hodinka, Antiviral Susceptibility Testing Using DNA-DNA Hybridization. J.C. Wilbur and M.S. Urdea, Quantitation of Viral Nucleic Acids Using Branched DNA (bDNA) Signal Amplification. I. Bronstein and C.E.M. Olesen, Chemiluminescence Methods. B.J. McCreedy, Polymerase Chain Reaction. F. Ferre, P. Pezzoli, E. Buxton, C. Duffy, A. Marchese, and A. Diagle, Quantitation of RNA Targets Using the Polymerase Chain Reaction. J.B. Mahony and M.A. Chernesky, Multiplex Polymerase Chain Reaction (M-PCR). G.J. Nuovo, PCR in Situ Hybridization. R. Sooknanan, B. van Gemen, and L.T. Malek, Nucleic Acid Sequence Based Amplification (NASBA(tm)). S.S.Ghosh, E. Fahy, and T.R. Gingeras, The Self-Sustained Sequence Replication (3SR) Reaction and its Application in Clinical Diagnostics and Molecular Biology. J.D. Burczak, S. Ching, H.-Y. Hu, and H.H. Lee, Ligase Chain Reaction (LCR) for the Detection of Infectious Agents. G. T. Walker, et al., A Chemiluminescent DNA Probe Test Based upon Strand Displacement Amplification (SDA). D.M. Schuster, M.S. Berninger, and A. Rashtchian, Ligation Activated Transcription Amplification: Amplification and Detection of Human Papillomaviruses. Subject Index.

Details

No. of pages:
386
Language:
English
Copyright:
© 1995
Published:
Imprint:
Academic Press
Print ISBN:
9780127489209
Electronic ISBN:
9780080536897

Reviews

@qu:"A particular strength of many of the chapters is the emphasis which has been placed on identifying problems that can arise when introducing and performing the tests and discussion of relative advantages and disadvantages... The clearly presented protocols should enable many research and development departments to develop in house assays." @source:--D. Cubitt, in THE JOURNAL OF CLINICAL PATHOLOGY