Molecular Mechanisms in the Control of Gene Expression

1st Edition - January 1, 1976
Editors: Donald P. Nierlich, W.J. Rutter, C. Fred Fox
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 7 3 9 3 - 8

Molecular Mechanisms in the Control of Gene Expression documents the proceedings of the ICN-UCLA conference on Molecular Mechanisms in the Control of Gene Expression, organized… Read more

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Molecular Mechanisms in the Control of Gene Expression documents the proceedings of the ICN-UCLA conference on Molecular Mechanisms in the Control of Gene Expression, organized through the Molecular Biology Institute of UCLA, held in Keystone, Colorado, 21-26 March 1976. The conference focused on three topics: the action of repressors on specific nucleotide sequences in DNA; how DNA and histones are intertwined in eucaryotic chromosomes; and in the development of new techniques that appear to lift genes from complex genomes. The volume contains 65 chapters organized into nine parts. The papers in Part I examine the organization of prokaryotic and eukaryotic chromosomes. Part II presents studies on the interaction of RNA a polymerase and regulatory molecules with defined DNA sites. Parts III and IV focus on RNA polymerases of eukaryotes and the regulation of transcription in eukaryotic systems, respectively. Part V contains papers dealing with nucleic acid sequences, transcription, and processing. Part VI covers cellular aspects in the study of gene expression. Part VII takes up cloning while Part VIII is devoted to genetic analysis through restriction mapping and molecular cloning. Finally, Part IX summarizes the recent progress reported at the conference and also indicates some of the limitations that can be placed upon interpretation of data.

Preface

I. Organization of Prokaryotic and Eukaryotic Chromosomes

1. The Structure of Chromatin and Its Reconstruction

2. X-Ray Diffraction From Isolated Repeat Units of Chromatin

3. Nucleoprotein Core Particles in Chromatin Subunits: Existence of a Complex of Eight Histones and 140 Nucleotide Pairs of DNA

4. Histone 2A-2B-4 Interactions within Chromatin

5. The Site of Trimethylpsoralen Cross-Linking in Chromatin

6. The Isolation, Characterization and Suggested Structure of Euchromatic Segments from Mouse TLT Hepatoma Chromatin

7. Chromatin Assembly. Sites of Association of Newly Synthesized Chromatin Proteins on DNA

8. Autoradiographic Visualization and Sedimentation Properties of Unfolded Bacterial Nucleoid DNA

9. Thermoplasma Acidophilum: Studies on a Prokaryote That Contains a Histone-like Protein

10. Factors Which Control DNA Packaging May Influence the Control of Transcription

II. Interaction of RNA Polymerase and Regulatory Molecules with Defined DNA Sites

11. Transcriptional and Translational (?) Control of the λ Repressor Gene (cl)

12. Regulation of Transcription Initiation and Termination in the Control of Expression of the Tryptophan Operon of E. coli

13. Positive Control of the Temporal Program of Bacteriophage SP01 Gene Expression by Phage and Host Specified Subunits of RNA Polymerase

14. DNA-Binding Specificity of a Positively Regulating RNA Polymerase

15. Characterization of the Promoter of the T4 tRNA Operon

16. Chemical Modification of Supercoiled DNA: Effect on the Rate of Transcription

17. The Interaction of Chemically Synthesized 21 Base Pair Lac Operator with the Lac Repressor

18. Synthesis of Lac and λ Operator DNA Sequences

19. Reconstitution of Operator DNA-Active Lactose Repressor from Subunits

20. Electron Microscopy of Glutamine Synthetase-DNA Interactions

21. Regulation of Transcription by Peptide Antibiotics

III. RNA Polymerases of Eukaryotes: Transcription and Specificity

22. Characteristics of an In Vitro System Which Transcribes Viral RNA from Chromatin

23. Selective Transcription of the 5S RNA Genes in Isolated Chromatin by RNA Polymerase III

24. Oocyte RNA Polymerase of Xenopus Laevis: In Vitro Transcription of Amplified Ribosomal DNA

25. Localization of RNA Polymerase on Drosophila Polytene Chromosomes by Indirect Immunofluorescence

26. Subclasses of RNA Polymerase in the Urchin Embryo

27. Multiple RNA Polymerases from Acanthamoeba castellanii: Lack of Alteration in Subunit Architecture and Levels during Encystment

28. DNA-Dependent RNA Polymerases from Higher Plants

29. Studies on the Inhibition of Transcription by the Hepatocarcinogen N-Hydroxy-2-Acetylaminofluorene

IV. Regulation of Transcription in Eukaryotic Systems

30. The Relationship between Chromatin Structure and Transcription

31. The Synthesis, Isolation, Amplification, and Transcription of the Ovalbumin Gene

32. Kinetics of Ovalbumin and Conalbumin mRNA Induction by Estrogen and Progesterone

33. DNA-Dependent RNA Polymerase I and II Activation by 17ß-Estradiol-Receptor Complex on Liver Chromatin from Immature Chicks

34. Effect of Estrogen on Gene Expression: Vitellogenin Synthesis May Be Regulated at the Level of Both Transcription and Translation

35. Selective Transcription of the Euglena gracilis Chloroplast Chromosome In Vitro

36. Products of Cell-Free RNA Synthesis Using Conditions Preventing Initiation and Processing

V. Nucleic Acid Sequences, Transcription and Processing

37. Sequences of SV40 DNA

38. Complementary Sequences in Heterogeneous Nuclear RNA

39. Purification of Globin mRNA and Detection of Its Presumptive Precursor

40. The Complexity of Nuclear RNA and Messenger RNA m the Uninduced Friend Cell

41. A Comparison of the Sizes of Messenger RNAs Coding for Lysozyme (Gene 3.5) in T7-Infected RNase III+ and RNase III- Strains of Escherichia coli

42. Processing of Ribosomal RNA in E. coli

43. RNA Unwinding Proteins: Mechanism of Acrion and Apparent Cellular Location

44. Polynucleoüde Binding Properties of E. coli Ribosomal Protein SI

45. Kinetic Studies on the Hybridization of RNA to Double Stranded DNA

VI. Cellular Aspects in the Study of Gene Expression

46. Nuclear and Cytoplasmic Roles in Determination of Cellular Phenotype

47. Nuclear Transplantation with Mammalian Cells

48. Red Cell Microinjection of Transfer RNA Molecules

VU. Cloning and Cloning Vehicles

49. Construction and Characterization of Cloning Vehicles

50. A Mutation Amplifying the Genes Carried by the pi-Histidine Plasmid

51. The Fidelity of Replication of Mouse Mitochondrial DNA-pSC101 Recombinant Plasmids Grown in E. coli K12

52. Genetic Selections and the Cloning of Prokaryotic and Eukaryotic Genes

53. The Production of Proteins by Bacterial Plasmids Containing Eukaryotic DNA Fragments

54. In Vitro Synthesis and Molecular Cloning of Eukaryotic Structural Genes

55. Insertion of Rabbit Globin Sequences into E. coli Plasmids

VIII. Genetic Analysis through Restriction Mapping and Molecular Cloning

56. Cloning of Drosophila melanogaster ECORI DNA Fragment

57. Initial Studies of Sea Urchin DNA Sequence Organization by Molecular Cloning

58. Use of Molecular Cloning in the Genetic and Functional Analyses of the Plasmid F

59. Cloning of the Ribosomal RNA Genes of Yeast

60. Physical Mapping of SV40-Lambda Hybrid Genomes

61. Interspersion of Inverted and Middle Repeated Sequences within the Genome of the Silkworm, Bombyx mori

62. Organization of the Ribosomal Genes of Dictyostelium

63. Physical Mapping of Bacteriophage DNA by Exonuclease III and Endodeoxyribonuclease BAMH1

64. Isolation and Transcription of T4 DNA Fragments Containing the T4 tRNA Genes

IX. Summing up

65. Perspective and Trends in Gene Expression

Author Index

Subject Index