Molecular Mechanisms in the Control of Gene Expression

Molecular Mechanisms in the Control of Gene Expression

1st Edition - January 1, 1976

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  • Editors: Donald P. Nierlich, W.J. Rutter, C. Fred Fox
  • eBook ISBN: 9781483273938

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Molecular Mechanisms in the Control of Gene Expression documents the proceedings of the ICN-UCLA conference on Molecular Mechanisms in the Control of Gene Expression, organized through the Molecular Biology Institute of UCLA, held in Keystone, Colorado, 21-26 March 1976. The conference focused on three topics: the action of repressors on specific nucleotide sequences in DNA; how DNA and histones are intertwined in eucaryotic chromosomes; and in the development of new techniques that appear to lift genes from complex genomes. The volume contains 65 chapters organized into nine parts. The papers in Part I examine the organization of prokaryotic and eukaryotic chromosomes. Part II presents studies on the interaction of RNA a polymerase and regulatory molecules with defined DNA sites. Parts III and IV focus on RNA polymerases of eukaryotes and the regulation of transcription in eukaryotic systems, respectively. Part V contains papers dealing with nucleic acid sequences, transcription, and processing. Part VI covers cellular aspects in the study of gene expression. Part VII takes up cloning while Part VIII is devoted to genetic analysis through restriction mapping and molecular cloning. Finally, Part IX summarizes the recent progress reported at the conference and also indicates some of the limitations that can be placed upon interpretation of data.

Table of Contents

  • Preface

    I. Organization of Prokaryotic and Eukaryotic Chromosomes

    1. The Structure of Chromatin and Its Reconstruction

    2. X-Ray Diffraction From Isolated Repeat Units of Chromatin

    3. Nucleoprotein Core Particles in Chromatin Subunits: Existence of a Complex of Eight Histones and 140 Nucleotide Pairs of DNA

    4. Histone 2A-2B-4 Interactions within Chromatin

    5. The Site of Trimethylpsoralen Cross-Linking in Chromatin

    6. The Isolation, Characterization and Suggested Structure of Euchromatic Segments from Mouse TLT Hepatoma Chromatin

    7. Chromatin Assembly. Sites of Association of Newly Synthesized Chromatin Proteins on DNA

    8. Autoradiographic Visualization and Sedimentation Properties of Unfolded Bacterial Nucleoid DNA

    9. Thermoplasma Acidophilum: Studies on a Prokaryote That Contains a Histone-like Protein

    10. Factors Which Control DNA Packaging May Influence the Control of Transcription

    II. Interaction of RNA Polymerase and Regulatory Molecules with Defined DNA Sites

    11. Transcriptional and Translational (?) Control of the λ Repressor Gene (cl)

    12. Regulation of Transcription Initiation and Termination in the Control of Expression of the Tryptophan Operon of E. coli

    13. Positive Control of the Temporal Program of Bacteriophage SP01 Gene Expression by Phage and Host Specified Subunits of RNA Polymerase

    14. DNA-Binding Specificity of a Positively Regulating RNA Polymerase

    15. Characterization of the Promoter of the T4 tRNA Operon

    16. Chemical Modification of Supercoiled DNA: Effect on the Rate of Transcription

    17. The Interaction of Chemically Synthesized 21 Base Pair Lac Operator with the Lac Repressor

    18. Synthesis of Lac and λ Operator DNA Sequences

    19. Reconstitution of Operator DNA-Active Lactose Repressor from Subunits

    20. Electron Microscopy of Glutamine Synthetase-DNA Interactions

    21. Regulation of Transcription by Peptide Antibiotics

    III. RNA Polymerases of Eukaryotes: Transcription and Specificity

    22. Characteristics of an In Vitro System Which Transcribes Viral RNA from Chromatin

    23. Selective Transcription of the 5S RNA Genes in Isolated Chromatin by RNA Polymerase III

    24. Oocyte RNA Polymerase of Xenopus Laevis: In Vitro Transcription of Amplified Ribosomal DNA

    25. Localization of RNA Polymerase on Drosophila Polytene Chromosomes by Indirect Immunofluorescence

    26. Subclasses of RNA Polymerase in the Urchin Embryo

    27. Multiple RNA Polymerases from Acanthamoeba castellanii: Lack of Alteration in Subunit Architecture and Levels during Encystment

    28. DNA-Dependent RNA Polymerases from Higher Plants

    29. Studies on the Inhibition of Transcription by the Hepatocarcinogen N-Hydroxy-2-Acetylaminofluorene

    IV. Regulation of Transcription in Eukaryotic Systems

    30. The Relationship between Chromatin Structure and Transcription

    31. The Synthesis, Isolation, Amplification, and Transcription of the Ovalbumin Gene

    32. Kinetics of Ovalbumin and Conalbumin mRNA Induction by Estrogen and Progesterone

    33. DNA-Dependent RNA Polymerase I and II Activation by 17ß-Estradiol-Receptor Complex on Liver Chromatin from Immature Chicks

    34. Effect of Estrogen on Gene Expression: Vitellogenin Synthesis May Be Regulated at the Level of Both Transcription and Translation

    35. Selective Transcription of the Euglena gracilis Chloroplast Chromosome In Vitro

    36. Products of Cell-Free RNA Synthesis Using Conditions Preventing Initiation and Processing

    V. Nucleic Acid Sequences, Transcription and Processing

    37. Sequences of SV40 DNA

    38. Complementary Sequences in Heterogeneous Nuclear RNA

    39. Purification of Globin mRNA and Detection of Its Presumptive Precursor

    40. The Complexity of Nuclear RNA and Messenger RNA m the Uninduced Friend Cell

    41. A Comparison of the Sizes of Messenger RNAs Coding for Lysozyme (Gene 3.5) in T7-Infected RNase III+ and RNase III- Strains of Escherichia coli

    42. Processing of Ribosomal RNA in E. coli

    43. RNA Unwinding Proteins: Mechanism of Acrion and Apparent Cellular Location

    44. Polynucleoüde Binding Properties of E. coli Ribosomal Protein SI

    45. Kinetic Studies on the Hybridization of RNA to Double Stranded DNA

    VI. Cellular Aspects in the Study of Gene Expression

    46. Nuclear and Cytoplasmic Roles in Determination of Cellular Phenotype

    47. Nuclear Transplantation with Mammalian Cells

    48. Red Cell Microinjection of Transfer RNA Molecules

    VU. Cloning and Cloning Vehicles

    49. Construction and Characterization of Cloning Vehicles

    50. A Mutation Amplifying the Genes Carried by the pi-Histidine Plasmid

    51. The Fidelity of Replication of Mouse Mitochondrial DNA-pSC101 Recombinant Plasmids Grown in E. coli K12

    52. Genetic Selections and the Cloning of Prokaryotic and Eukaryotic Genes

    53. The Production of Proteins by Bacterial Plasmids Containing Eukaryotic DNA Fragments

    54. In Vitro Synthesis and Molecular Cloning of Eukaryotic Structural Genes

    55. Insertion of Rabbit Globin Sequences into E. coli Plasmids

    VIII. Genetic Analysis through Restriction Mapping and Molecular Cloning

    56. Cloning of Drosophila melanogaster ECORI DNA Fragment

    57. Initial Studies of Sea Urchin DNA Sequence Organization by Molecular Cloning

    58. Use of Molecular Cloning in the Genetic and Functional Analyses of the Plasmid F

    59. Cloning of the Ribosomal RNA Genes of Yeast

    60. Physical Mapping of SV40-Lambda Hybrid Genomes

    61. Interspersion of Inverted and Middle Repeated Sequences within the Genome of the Silkworm, Bombyx mori

    62. Organization of the Ribosomal Genes of Dictyostelium

    63. Physical Mapping of Bacteriophage DNA by Exonuclease III and Endodeoxyribonuclease BAMH1

    64. Isolation and Transcription of T4 DNA Fragments Containing the T4 tRNA Genes

    IX. Summing up

    65. Perspective and Trends in Gene Expression

    Author Index

    Subject Index

Product details

  • No. of pages: 668
  • Language: English
  • Copyright: © Academic Press 1976
  • Published: January 1, 1976
  • Imprint: Academic Press
  • eBook ISBN: 9781483273938

About the Editors

Donald P. Nierlich

W.J. Rutter

C. Fred Fox

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