Molecular Imaging

Molecular Imaging

Principles and Practice

2nd Edition - August 2, 2021

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  • Editors: Brian Ross, Sanjiv Gambhir
  • Hardcover ISBN: 9780128163863
  • eBook ISBN: 9780128163870

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The detection and measurement of the dynamic regulation and interactions of cells and proteins within the living cell are critical to the understanding of cellular biology and pathophysiology. The multidisciplinary field of molecular imaging of living subjects continues to expand with dramatic advances in chemistry, molecular biology, therapeutics, engineering, medical physics and biomedical applications. Molecular Imaging: Principles and Practice, Volumes 1 and 2, Second Edition provides the first point of entry for physicians, scientists, and practitioners. This authoritative reference book provides a comprehensible overview along with in-depth presentation of molecular imaging concepts, technologies and applications making it the foremost source for both established and new investigators, collaborators, students and anyone interested in this exciting and important field.

Key Features

  • The most authoritative and comprehensive resource available in the molecular-imaging field, written by over 170 of the leading scientists from around the world who have evaluated and summarized the most important methods, principles, technologies and data
  • Concepts illustrated with over 600 color figures and molecular-imaging examples
  • Chapters/topics include, artificial intelligence and machine learning, use of online social media, virtual and augmented reality, optogenetics, FDA regulatory process of imaging agents and devices, emerging instrumentation, MR elastography, MR fingerprinting, operational radiation safety, multiscale imaging and uses in drug development
  • This edition is packed with innovative science, including theranostics, light sheet fluorescence microscopy, (LSFM), mass spectrometry imaging, combining in vitro and in vivo diagnostics, Raman imaging, along with molecular and functional imaging applications
  • Valuable applications of molecular imaging in pediatrics, oncology, autoimmune, cardiovascular and CNS diseases are also presented
  • This resource helps integrate diverse multidisciplinary concepts associated with molecular imaging to provide readers with an improved understanding of current and future applications


Molecular imaging basic scientists, molecular biologists, oncologists, functional and nuclear medicine imaging, radiologists with molecular imaging background/interest

Table of Contents

  • Volume 1
    1. General Principles of Molecular Imaging
    Brian D. Ross and Sanjiv Sam Gambhir
    Part I: Molecular Imaging Technologies
    2. Positron Emission TomographyeMagnetic Resonance Imaging
    Farshad Moradi, Ryan L. Brunsing, Vipul R. Sheth and Andrei Iagaru
    3. SPECT and SPECT/CT
    Brian F. Hutton, Marlies C. Goorden and Freek J. Beekman
    4. Principles of Micro X-ray Computed Tomography
    Cristian T. Badea
    5. Small-Animal SPECT, SPECT/CT, and SPECT/MRI
    Ling-Jian Meng and Neal H. Clinthorne
    6. Instrumentation and Methods to Combine Small-Animal PET With Other Imaging Modalities
    Andrew N. Groll and Craig S. Levin
    7. Functional Imaging Using Bioluminescent Reporter Genes in Living Subjects
    Scott K. Lyons, Kevin P. Francis and Christopher H. Contag
    8. Principles and Practice of Intraoperative Fluorescence Imaging
    Dimitris Gorpas, Vasilis Ntziachristos and Jie Tian
    9. Molecular Endoscopic Imaging in Cancer
    Timo Rath, Markus F. Neurath and Raja Atreya
    10. Intravital Microscopy
    Shariq M. Usmani and Thorsten R. Mempel
    11. Ultrasound
    F. Stuart Foster and Emmanuel Cherin
    12. Hybrid PET-CT-Ultrasound Imaging
    Bertrand Tavitian and Mailyn Perez-Liva
    13. Photoacoustic Molecular Imaging: Principles and Practice
    Junjie Yao and Lihong V. Wang
    14. Inhaled Gas Magnetic Resonance Imaging: Advances, Applications, Limitations, and New Frontiers
    Alexander M. Matheson, Caleb Thompson and Grace Parraga
    15. Magnetic Particle Imaging for Vascular, Cellular and Molecular Imaging
    Prashant Chandrasekharan, Zhi Wei Tay, Xinyi Y. Zhou, Elaine Y. Yu, Barry K.L. Fung, Caylin Colson, Benjamin D. Fellows, Yao Lu, Quincy Huynh, Chinmoy Saayujya, Paul Keselman, Daniel Hensley, Kuan Lu, Ryan Orendorff, Justin Konkle, Emine Ulku Saritas, Bo Zheng, Patrick Goodwill and Steven Conolly
    16. Optogenetics
    Maria G. Paez Segala and Loren L. Looger
    17. Imaging Mass Spectrometry
    Richard R. Drake, Danielle A. Scott and Peggi M. Angel
    18. CEST (Chemical Exchange Saturation Transfer) MR Molecular Imaging
    Guanshu Liu and Peter C.M. van Zijl
    19. Molecular Imaging Using Raman Scattering
    Stefan Harmsen and Sanjiv Sam Gambhir
    20. Synchrotron-Based Dynamic Lung Imaging
    Stephen Dubsky
    21. Light-Sheet Fluorescence Microscopy for Multiscale Biological Imaging
    Jae-Byum Chang, Ruixuan Gao and Fei Chen
    22. Cerenkov Imaging
    Edwin C. Pratt, Ryo Tamura and Jan Grimm
    23. Near-Infrared II Optical Imaging
    Shuqing He and Zhen Cheng
    Part II: Chemistry of Molecular Imaging
    24. Chemistry of Molecular Imaging: An Overview
    Silvio Aime, Fabio Travagin, Enzo Terreno and Giovanni B. Giovenzana
    25. PET Radiochemistry
    Henry F. VanBrocklin
    26. SPECT Radiochemistry
    Theodosia Maina and Mathew Thakur
    27. The Chemistry in Surface Functionalization of Nanoparticles for Molecular Imaging 493
    Xianchuang Zheng, Joanna Wang and Jianghong Rao
    28. Newer Bioconjugation Methods
    Ahmet Krasniqi, Catarina Xavier and Nick Devoogdt
    29. Targeted Antibodies and Peptides
    Michael R. Lewis, Cathy S. Cutler and Silvia S. Jurisson
    30. Antibody-Based Molecular Imaging
    Kathryn M. Tully, Veronica L. Nagle, Joshua A. Korsen and Jason S. Lewis
    31. Hyperpolarized Magnetic Resonance With Dissolution Dynamic Nuclear Polarization: Principles and Applications
    Jan H. Ardenkjaer-Larsen
    32. Magnetic Resonance Imaging Agents
    Cuihua Wang, Elisenda Rodriguez and John W. Chen
    33. Optical Imaging Agents
    Luisa Ottobrini, Cristina Martelli and Giovanni Lucignani
    34. Photoacoustic Molecular Imaging With Exogenous Agents
    Liming Nie, Yajing Liu and Xiaogyuan Chen
    35. Ultrasound Contrast Agents
    Mark A. Borden, Paul A. Dayton, Connor Slagle and Rachel W. Walmer
    36. Theranostics: Agents for Diagnosis and Therapy
    Fay Nicolson and Moritz F. Kircher
    37. Magnetic Nanoparticles
    Ahmad Amirshaghaghi, Zhiliang Cheng, Lee Josephson and Andrew Tsourkas
    38. Aptamers for Molecular Imaging
    Fre´de´ric Duconge´
    39. Nonclinical Product Developmental Strategies, Safety Considerations and Toxicity Profiles of Medical Imaging and Radiopharmaceuticals Products
    Adebayo Laniyonu, Yanli Ouyang, Jonathan Cohen, Sunny Awe, Olayinka Dina, Siham Biade, Sally Hargus and Tushar Kokate
    40. Intraoperative Molecular Imaging Agents
    Stan van Keulen and Eben L. Rosenthal

    Volume 2
    Part III: Molecular Imaging in Cell and Molecular Biology
    41. Protein Engineering for Molecular Imaging
    Anna M. Wu
    42. Phage Display for Imaging Agent Development
    Jessica Hung, Fred Reynolds and Kimberly A. Kelly
    43. Molecular Imaging of Gene Therapy
    Ramasamy Paulmurugan and Tarik F. Massoud
    44. Cell Surveillance Using Magnetic Resonance Imaging
    Brooke M. Helfer and Jeff W.M. Bulte
    45. Tumor Vasculature
    Susan Notohamiprodjo, Zohreh Varasteh, Ambros J. Beer, Gang Niu, Xiaoyuan (Shawn) Chen, Wolfgang Weber and Markus Schwaiger
    46. Imaging Hypoxia
    Hong Yuan, Gregory M. Palmer and Mark W. Dewhirst
    47. Molecular Imaging of ProteineProtein Interactions and Protein Folding
    Tarik F. Massoud and Ramasamy Paulmurugan
    48. Molecular Imaging of Cellular Signaling Pathways
    Kathryn E. Luker
    49. Exosome Imaging
    Do Won Hwang, Minseok Suh and Dong Soo Lee
    50. Gene-Editing Technologies and Applications for Molecular Imaging
    Gary D. Luker
    51. Reporter Genes for Ultrasound and MRI
    Mikhail G. Shapiro
    52. Theranostic Approaches Using Live Bacteria
    Jung-Joon Min, Mai Thi-Quynh Duong, Thangam Ramar, Sung-Hwan You and Sae-Ryung Kang
    Part IV: Applications of Molecular Imaging Oncology
    53. Molecular and Functional Imaging and Theranostics of the Tumor Microenvironment
    Marie-France Penet, Samata Kakkad, Jesus Pacheco-Torres, Santosh Bharti, Balaji Krishnamachary and Zaver M. Bhujwalla
    54. Novel Magnetic Resonance Imaging and Positron Emission Tomography in the RT Planning and Assessment of Response of Malignant Gliomas
    David Kamson and Christina Tsien
    55. PET Diagnosis and Response Monitoring in Oncology
    Richard L. Wahl and Rodney J. Hicks
    56. Spectroscopic MRI for Brain Tumor Imaging
    Hyunsuk Shim
    57. Cancer Detection and Quantification of Treatment Response Using Diffusion-Weighted MRI
    Brian D. Ross and Thomas L. Chenevert
    58. Prostate Cancer Theranostics
    Steven P. Rowe, Mohammad S. Sadaghiani, Rudolf A. Werner, Takahiro Higuchi, Thorsten Derlin, Lilja B. Solnes and Martin G. Pomper
    59. Pediatric Molecular Imaging
    Heike E. Daldrup-Link
    60. Imaging Myocardial Metabolism
    Robert J. Gropler
    61. Congestive Heart Failure
    Stephan G. Nekolla, Antti Saraste, Hendrik Sager, Marcus R. Makowski and Markus Schwaiger
    62. Molecular Imaging of Atherosclerosis
    Eric A. Osborn, Mazen Albaghdadi, Peter Libby and Farouc A. Jaffer
    63. Thrombosis and Embolism
    Reza Hajhosseiny, Claudia Prieto, Haikun Qi, Alkystis Phinikaridou and Rene´ M. Botnar
    64. Molecular Imaging of Stem Cell Therapy in Ischemic Cardiomyopathy
    Mark Chandy and Joseph C. Wu
    Central Nervous System
    65. Central Nervous System Molecular Imaging
    Lilja B. Solnes, Andreas H. Jacobs, Jennifer M. Coughlin, Yong Du, Reema Goel, Dima A. Hammoud and Martin G. Pomper
    66. Applications, Advances, and Limitations of Molecular Imaging of Brain Receptors
    Paul Cumming, Gerhard Gru¨nder, Zabecca Brinson and Dean F. Wong
    67. PET and SPECT Imaging of Neurodegenerative Diseases
    Alexander Drzezga, Ge´rard N. Bischof, Kathrin Giehl and Thilo van Eimeren
    68. PET Imaging of Neuroinflammation
    Aisling M. Chaney, Emily M. Deal, Isaac M. Jackson and Michelle L. James
    Autoimmunity and Immunology
    69. Identifying Musculoskeletal Pain Generators Using Molecular Imaging
    Daehyun Yoon, Feliks Kogan, Garry Gold and Sandip Biswal
    70. Molecular Imaging of Autoimmune Diseases
    Alberto Signore, Arianna Di Paolo and Filippo Galli
    71. Molecular Imaging of Diabetes
    Lieke Joosten, Marti Boss, Tom Jansen, Maarten Brom, Mijke Buitinga, Erik Aarntzen, Olof Eriksson, Lars Johansson, Bastiaan de Galan and Martin Gotthardt
    72. Imaging in Asthma
    Avanti Gulhane and Delphine L. Chen
    73. Molecular Imaging of Fibrosis
    Iris Y. Zhou, Sydney B. Montesi, Eman A. Akam and Peter Caravan
    74. Imaging of Bacterial Infections
    Alvaro A. Ordon˜ez and Sanjay K. Jain
    Part V: Molecular Imaging in Drug Evaluation
    75. Molecular and Functional Imaging in Central Nervous System Drug Development
    Basil Ku¨nnecke, Michael Honer, Gennaro Pagano and Markus Rudin
    76. PET Imaging in Cancer Clinical Trials
    David A. Mankoff
    77. Magnetic Resonance Imaging: Cardiovascular Applications for Clinical Trials
    Chun Yuan, Zach Miller and Xue-Qiao Zhao
    78. Imaging of Gene and Cell-Based Therapies: Basis and Clinical Trials
    Andreas H. Jacobs, Sonja Schelhaas, Thomas Viel, Yannic Waerzeggers, Alexandra Winkeler, Bastian Zinnhardt and Juri Gelovani
    Part VI: Image Quantitation/Analytics
    79. Virtual and Augmented Reality Technologies in Molecular and Anatomical Imaging
    Suman B. Mondal and Samuel Achilefu
    80. Quantification of Radiotracer Uptake Into Tissue
    Michael M. Graham
    81. Pharmacokinetic Modeling
    Sung-Cheng (Henry) Huang
    82. Online Social Media: Concepts and Practices for Molecular Imaging Professionals
    Chadwick L. Wright, Michelle I. Knopp and Michael V. Knopp
    83. The Regulatory Process for Imaging Agents and Devices
    Matthew F. Covington, Sally W. Schwarz and John M. Hoffman
    84. Operational Radiation Safety for PET-CT, SPECT-CT, and Cyclotron Facilities
    Pat Zanzonico
    85. Bridging Spatial Scales From Radiographic Images to Cellular and Molecular Properties in Cancers
    Natarajan Raghunand and Robert A. Gatenby
    86. AI/Machine Learning in Medical Imaging
    Maryellen L. Giger
    87. Retrospective Registration in Molecular Imaging
    Brian D. Ross, Thomas L. Chenevert and Charles R. Meyer
    88. Image Processing Analytics: Enhancements and Segmentation
    Lubomir Hadjiiski, Ravi Samala and Heang-Ping Chan
    89. Magnetic Resonance Fingerprinting: Basic Concepts and Applications in Molecular Imaging
    Kathleen Ropella-Panagis and Nicole Seiberlich
    90. MR Elastography
    Patrick J. Navin, Sudhakar Kundapur Venkatesh and Richard L. Ehman

Product details

  • No. of pages: 1868
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: August 2, 2021
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128163863
  • eBook ISBN: 9780128163870

About the Editors

Brian Ross

Dr. Ross is the Director of the Center for Molecular Imaging at the University of Michigan, which provides state-of-the-art imaging services for the research community and offering a broad range of imaging systems (two IVIS bioluminescent/fluorescent imaging systems, an Agilent 7 Tesla horizontal bore MRI system, a Siemens PET/CT, a Trifoil Imaging SPECT/CT, and a Bruker CT system). As part of CMI, his research helps to develop applications for imaging to allow for investigations of cancer growth and response to therapy including noninvasive detection of molecular signaling events in living tissue.

Affiliations and Expertise

Roger A. Berg Research Professor of Radiology; Director, Center for Molecular Imaging; Associate Chair for Basic Science Research; Professor of Biological Chemistry; University of Michigan School of Medicine, Ann Arbor, Michigan, USA

Sanjiv Gambhir

Dr. Gambhir served as the Director of the Molecular Imaging Program at Stanford (MIPS) and Canary Center at Stanford for Cancer Early Detection. He has authored over 600 publications, has over 40 patents pending or granted, has been featured on the cover of over 25 journals including the Nature Series, Science, and Science Translational Medicine. He was on the editorial board of several journals including Nano Letters, Nature Clinical Practice Oncology, and Science Translational Medicine. He mentored post-doctoral fellows and graduate students from diverse disciplines. His research focused on the development of imaging assays to monitor fundamental cellular/molecular events in living subjects with an emphasis on the detection and management of cancer. A particular interest of his research and lab was early cancer detection including combining in vivo and in vitro diagnostics.

Affiliations and Expertise

Virginia & D.K. Ludwig Professor of Radiology and Bioengineering; Director, Molecular Imaging Program at Stanford (MIPS); Director, Canary Center for Cancer Early Detection at Stanford; Chief, Division of Nuclear Medicine; Stanford University School of Medicine, Stanford, California, USA

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  • Chin N. Mon May 30 2022

    e version of Molecular Imaging

    A very nice summary of the field of molecular imaging. A must-have book for those who would like to get a comprehensive view of how to apply the principles of molecular imaging to discover disease mechanism..