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Molecular Imaging - 2nd Edition - ISBN: 9780128163863, 9780128163870

Molecular Imaging

2nd Edition

Principles and Practice

Editors: Brian Ross Sanjiv Gambhir
Hardcover ISBN: 9780128163863
eBook ISBN: 9780128163870
Imprint: Academic Press
Published Date: 2nd August 2021
Page Count: 1868
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The detection and measurement of the dynamic regulation and interactions of cells and proteins within the living cell are critical to the understanding of cellular biology and pathophysiology. The multidisciplinary field of molecular imaging of living subjects continues to expand with dramatic advances in chemistry, molecular biology, therapeutics, engineering, medical physics and biomedical applications. Molecular Imaging: Principles and Practice, Volumes 1 and 2, Second Edition provides the first point of entry for physicians, scientists, and practitioners. This authoritative reference book provides a comprehensible overview along with in-depth presentation of molecular imaging concepts, technologies and applications making it the foremost source for both established and new investigators, collaborators, students and anyone interested in this exciting and important field.

Key Features

The most authoritative and comprehensive resource available in the molecular-imaging field, written by over 170 of the leading scientists from around the world who have evaluated and summarized the most important methods, principles, technologies and data
Concepts illustrated with over 600 color figures and molecular-imaging examples
Chapters/topics include, artificial intelligence and machine learning, use of online social media, virtual and augmented reality, optogenetics, FDA regulatory process of imaging agents and devices, emerging instrumentation, MR elastography, MR fingerprinting, operational radiation safety, multiscale imaging and uses in drug development
This edition is packed with innovative science, including theranostics, light sheet fluorescence microscopy, (LSFM), mass spectrometry imaging, combining in vitro and in vivo diagnostics, Raman imaging, along with molecular and functional imaging applications
Valuable applications of molecular imaging in pediatrics, oncology, autoimmune, cardiovascular and CNS diseases are also presented
This resource helps integrate diverse multidisciplinary concepts associated with molecular imaging to provide readers with an improved understanding of current and future applications


Molecular imaging basic scientists, molecular biologists, oncologists, functional and nuclear medicine imaging, radiologists with molecular imaging background/interest

Table of Contents

Volume 1
1. General Principles of Molecular Imaging
Brian D. Ross and Sanjiv Sam Gambhir
Part I: Molecular Imaging Technologies
2. Positron Emission TomographyeMagnetic Resonance Imaging
Farshad Moradi, Ryan L. Brunsing, Vipul R. Sheth and Andrei Iagaru
Brian F. Hutton, Marlies C. Goorden and Freek J. Beekman
4. Principles of Micro X-ray Computed Tomography
Cristian T. Badea
5. Small-Animal SPECT, SPECT/CT, and SPECT/MRI
Ling-Jian Meng and Neal H. Clinthorne
6. Instrumentation and Methods to Combine Small-Animal PET With Other Imaging Modalities
Andrew N. Groll and Craig S. Levin
7. Functional Imaging Using Bioluminescent Reporter Genes in Living Subjects
Scott K. Lyons, Kevin P. Francis and Christopher H. Contag
8. Principles and Practice of Intraoperative Fluorescence Imaging
Dimitris Gorpas, Vasilis Ntziachristos and Jie Tian
9. Molecular Endoscopic Imaging in Cancer
Timo Rath, Markus F. Neurath and Raja Atreya
10. Intravital Microscopy
Shariq M. Usmani and Thorsten R. Mempel
11. Ultrasound
F. Stuart Foster and Emmanuel Cherin
12. Hybrid PET-CT-Ultrasound Imaging
Bertrand Tavitian and Mailyn Perez-Liva
13. Photoacoustic Molecular Imaging: Principles and Practice
Junjie Yao and Lihong V. Wang
14. Inhaled Gas Magnetic Resonance Imaging: Advances, Applications, Limitations, and New Frontiers
Alexander M. Matheson, Caleb Thompson and Grace Parraga
15. Magnetic Particle Imaging for Vascular, Cellular and Molecular Imaging
Prashant Chandrasekharan, Zhi Wei Tay, Xinyi Y. Zhou, Elaine Y. Yu, Barry K.L. Fung, Caylin Colson, Benjamin D. Fellows, Yao Lu, Quincy Huynh, Chinmoy Saayujya, Paul Keselman, Daniel Hensley, Kuan Lu, Ryan Orendorff, Justin Konkle, Emine Ulku Saritas, Bo Zheng, Patrick Goodwill and Steven Conolly
16. Optogenetics
Maria G. Paez Segala and Loren L. Looger
17. Imaging Mass Spectrometry
Richard R. Drake, Danielle A. Scott and Peggi M. Angel
18. CEST (Chemical Exchange Saturation Transfer) MR Molecular Imaging
Guanshu Liu and Peter C.M. van Zijl
19. Molecular Imaging Using Raman Scattering
Stefan Harmsen and Sanjiv Sam Gambhir
20. Synchrotron-Based Dynamic Lung Imaging
Stephen Dubsky
21. Light-Sheet Fluorescence Microscopy for Multiscale Biological Imaging
Jae-Byum Chang, Ruixuan Gao and Fei Chen
22. Cerenkov Imaging
Edwin C. Pratt, Ryo Tamura and Jan Grimm
23. Near-Infrared II Optical Imaging
Shuqing He and Zhen Cheng
Part II: Chemistry of Molecular Imaging
24. Chemistry of Molecular Imaging: An Overview
Silvio Aime, Fabio Travagin, Enzo Terreno and Giovanni B. Giovenzana
25. PET Radiochemistry
Henry F. VanBrocklin
26. SPECT Radiochemistry
Theodosia Maina and Mathew Thakur
27. The Chemistry in Surface Functionalization of Nanoparticles for Molecular Imaging 493
Xianchuang Zheng, Joanna Wang and Jianghong Rao
28. Newer Bioconjugation Methods
Ahmet Krasniqi, Catarina Xavier and Nick Devoogdt
29. Targeted Antibodies and Peptides
Michael R. Lewis, Cathy S. Cutler and Silvia S. Jurisson
30. Antibody-Based Molecular Imaging
Kathryn M. Tully, Veronica L. Nagle, Joshua A. Korsen and Jason S. Lewis
31. Hyperpolarized Magnetic Resonance With Dissolution Dynamic Nuclear Polarization: Principles and Applications
Jan H. Ardenkjaer-Larsen
32. Magnetic Resonance Imaging Agents
Cuihua Wang, Elisenda Rodriguez and John W. Chen
33. Optical Imaging Agents
Luisa Ottobrini, Cristina Martelli and Giovanni Lucignani
34. Photoacoustic Molecular Imaging With Exogenous Agents
Liming Nie, Yajing Liu and Xiaogyuan Chen
35. Ultrasound Contrast Agents
Mark A. Borden, Paul A. Dayton, Connor Slagle and Rachel W. Walmer
36. Theranostics: Agents for Diagnosis and Therapy
Fay Nicolson and Moritz F. Kircher
37. Magnetic Nanoparticles
Ahmad Amirshaghaghi, Zhiliang Cheng, Lee Josephson and Andrew Tsourkas
38. Aptamers for Molecular Imaging
Fre´de´ric Duconge´
39. Nonclinical Product Developmental Strategies, Safety Considerations and Toxicity Profiles of Medical Imaging and Radiopharmaceuticals Products
Adebayo Laniyonu, Yanli Ouyang, Jonathan Cohen, Sunny Awe, Olayinka Dina, Siham Biade, Sally Hargus and Tushar Kokate
40. Intraoperative Molecular Imaging Agents
Stan van Keulen and Eben L. Rosenthal

Volume 2
Part III: Molecular Imaging in Cell and Molecular Biology
41. Protein Engineering for Molecular Imaging
Anna M. Wu
42. Phage Display for Imaging Agent Development
Jessica Hung, Fred Reynolds and Kimberly A. Kelly
43. Molecular Imaging of Gene Therapy
Ramasamy Paulmurugan and Tarik F. Massoud
44. Cell Surveillance Using Magnetic Resonance Imaging
Brooke M. Helfer and Jeff W.M. Bulte
45. Tumor Vasculature
Susan Notohamiprodjo, Zohreh Varasteh, Ambros J. Beer, Gang Niu, Xiaoyuan (Shawn) Chen, Wolfgang Weber and Markus Schwaiger
46. Imaging Hypoxia
Hong Yuan, Gregory M. Palmer and Mark W. Dewhirst
47. Molecular Imaging of ProteineProtein Interactions and Protein Folding
Tarik F. Massoud and Ramasamy Paulmurugan
48. Molecular Imaging of Cellular Signaling Pathways
Kathryn E. Luker
49. Exosome Imaging
Do Won Hwang, Minseok Suh and Dong Soo Lee
50. Gene-Editing Technologies and Applications for Molecular Imaging
Gary D. Luker
51. Reporter Genes for Ultrasound and MRI
Mikhail G. Shapiro
52. Theranostic Approaches Using Live Bacteria
Jung-Joon Min, Mai Thi-Quynh Duong, Thangam Ramar, Sung-Hwan You and Sae-Ryung Kang
Part IV: Applications of Molecular Imaging Oncology
53. Molecular and Functional Imaging and Theranostics of the Tumor Microenvironment
Marie-France Penet, Samata Kakkad, Jesus Pacheco-Torres, Santosh Bharti, Balaji Krishnamachary and Zaver M. Bhujwalla
54. Novel Magnetic Resonance Imaging and Positron Emission Tomography in the RT Planning and Assessment of Response of Malignant Gliomas
David Kamson and Christina Tsien
55. PET Diagnosis and Response Monitoring in Oncology
Richard L. Wahl and Rodney J. Hicks
56. Spectroscopic MRI for Brain Tumor Imaging
Hyunsuk Shim
57. Cancer Detection and Quantification of Treatment Response Using Diffusion-Weighted MRI
Brian D. Ross and Thomas L. Chenevert
58. Prostate Cancer Theranostics
Steven P. Rowe, Mohammad S. Sadaghiani, Rudolf A. Werner, Takahiro Higuchi, Thorsten Derlin, Lilja B. Solnes and Martin G. Pomper
59. Pediatric Molecular Imaging
Heike E. Daldrup-Link
60. Imaging Myocardial Metabolism
Robert J. Gropler
61. Congestive Heart Failure
Stephan G. Nekolla, Antti Saraste, Hendrik Sager, Marcus R. Makowski and Markus Schwaiger
62. Molecular Imaging of Atherosclerosis
Eric A. Osborn, Mazen Albaghdadi, Peter Libby and Farouc A. Jaffer
63. Thrombosis and Embolism
Reza Hajhosseiny, Claudia Prieto, Haikun Qi, Alkystis Phinikaridou and Rene´ M. Botnar
64. Molecular Imaging of Stem Cell Therapy in Ischemic Cardiomyopathy
Mark Chandy and Joseph C. Wu
Central Nervous System
65. Central Nervous System Molecular Imaging
Lilja B. Solnes, Andreas H. Jacobs, Jennifer M. Coughlin, Yong Du, Reema Goel, Dima A. Hammoud and Martin G. Pomper
66. Applications, Advances, and Limitations of Molecular Imaging of Brain Receptors
Paul Cumming, Gerhard Gru¨nder, Zabecca Brinson and Dean F. Wong
67. PET and SPECT Imaging of Neurodegenerative Diseases
Alexander Drzezga, Ge´rard N. Bischof, Kathrin Giehl and Thilo van Eimeren
68. PET Imaging of Neuroinflammation
Aisling M. Chaney, Emily M. Deal, Isaac M. Jackson and Michelle L. James
Autoimmunity and Immunology
69. Identifying Musculoskeletal Pain Generators Using Molecular Imaging
Daehyun Yoon, Feliks Kogan, Garry Gold and Sandip Biswal
70. Molecular Imaging of Autoimmune Diseases
Alberto Signore, Arianna Di Paolo and Filippo Galli
71. Molecular Imaging of Diabetes
Lieke Joosten, Marti Boss, Tom Jansen, Maarten Brom, Mijke Buitinga, Erik Aarntzen, Olof Eriksson, Lars Johansson, Bastiaan de Galan and Martin Gotthardt
72. Imaging in Asthma
Avanti Gulhane and Delphine L. Chen
73. Molecular Imaging of Fibrosis
Iris Y. Zhou, Sydney B. Montesi, Eman A. Akam and Peter Caravan
74. Imaging of Bacterial Infections
Alvaro A. Ordon˜ez and Sanjay K. Jain
Part V: Molecular Imaging in Drug Evaluation
75. Molecular and Functional Imaging in Central Nervous System Drug Development
Basil Ku¨nnecke, Michael Honer, Gennaro Pagano and Markus Rudin
76. PET Imaging in Cancer Clinical Trials
David A. Mankoff
77. Magnetic Resonance Imaging: Cardiovascular Applications for Clinical Trials
Chun Yuan, Zach Miller and Xue-Qiao Zhao
78. Imaging of Gene and Cell-Based Therapies: Basis and Clinical Trials
Andreas H. Jacobs, Sonja Schelhaas, Thomas Viel, Yannic Waerzeggers, Alexandra Winkeler, Bastian Zinnhardt and Juri Gelovani
Part VI: Image Quantitation/Analytics
79. Virtual and Augmented Reality Technologies in Molecular and Anatomical Imaging
Suman B. Mondal and Samuel Achilefu
80. Quantification of Radiotracer Uptake Into Tissue
Michael M. Graham
81. Pharmacokinetic Modeling
Sung-Cheng (Henry) Huang
82. Online Social Media: Concepts and Practices for Molecular Imaging Professionals
Chadwick L. Wright, Michelle I. Knopp and Michael V. Knopp
83. The Regulatory Process for Imaging Agents and Devices
Matthew F. Covington, Sally W. Schwarz and John M. Hoffman
84. Operational Radiation Safety for PET-CT, SPECT-CT, and Cyclotron Facilities
Pat Zanzonico
85. Bridging Spatial Scales From Radiographic Images to Cellular and Molecular Properties in Cancers
Natarajan Raghunand and Robert A. Gatenby
86. AI/Machine Learning in Medical Imaging
Maryellen L. Giger
87. Retrospective Registration in Molecular Imaging
Brian D. Ross, Thomas L. Chenevert and Charles R. Meyer
88. Image Processing Analytics: Enhancements and Segmentation
Lubomir Hadjiiski, Ravi Samala and Heang-Ping Chan
89. Magnetic Resonance Fingerprinting: Basic Concepts and Applications in Molecular Imaging
Kathleen Ropella-Panagis and Nicole Seiberlich
90. MR Elastography
Patrick J. Navin, Sudhakar Kundapur Venkatesh and Richard L. Ehman


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© Academic Press 2021
2nd August 2021
Academic Press
Hardcover ISBN:
eBook ISBN:

About the Editors

Brian Ross

Dr. Ross is the Director of the Center for Molecular Imaging at the University of Michigan, which provides state-of-the-art imaging services for the research community and offering a broad range of imaging systems (two IVIS bioluminescent/fluorescent imaging systems, an Agilent 7 Tesla horizontal bore MRI system, a Siemens PET/CT, a Trifoil Imaging SPECT/CT, and a Bruker CT system). As part of CMI, his research helps to develop applications for imaging to allow for investigations of cancer growth and response to therapy including noninvasive detection of molecular signaling events in living tissue.

Affiliations and Expertise

Roger A. Berg Research Professor of Radiology; Director, Center for Molecular Imaging; Associate Chair for Basic Science Research; Professor of Biological Chemistry; University of Michigan School of Medicine, Ann Arbor, Michigan, USA

Sanjiv Gambhir

Dr. Gambhir served as the Director of the Molecular Imaging Program at Stanford (MIPS) and Canary Center at Stanford for Cancer Early Detection. He has authored over 600 publications, has over 40 patents pending or granted, has been featured on the cover of over 25 journals including the Nature Series, Science, and Science Translational Medicine. He was on the editorial board of several journals including Nano Letters, Nature Clinical Practice Oncology, and Science Translational Medicine. He mentored post-doctoral fellows and graduate students from diverse disciplines. His research focused on the development of imaging assays to monitor fundamental cellular/molecular events in living subjects with an emphasis on the detection and management of cancer. A particular interest of his research and lab was early cancer detection including combining in vivo and in vitro diagnostics.

Affiliations and Expertise

Virginia & D.K. Ludwig Professor of Radiology and Bioengineering; Director, Molecular Imaging Program at Stanford (MIPS); Director, Canary Center for Cancer Early Detection at Stanford; Chief, Division of Nuclear Medicine; Stanford University School of Medicine, Stanford, California, USA

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