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Molecular Imaging - 1st Edition - ISBN: 9780195177206, 9780080536873

Molecular Imaging

1st Edition

FRET Microscopy and Spectroscopy

Editors: Ammasi Periasamy Richard Day
Hardcover ISBN: 9780195177206
eBook ISBN: 9780080536873
Imprint: Academic Press
Published Date: 1st August 2005
Page Count: 312
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The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided.

The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.


Biochemists, molecular and cell biologists.

Table of Contents

Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy - Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization


No. of pages:
© Academic Press 2005
1st August 2005
Academic Press
Hardcover ISBN:
eBook ISBN:

About the Editors

Ammasi Periasamy

Richard Day


"This is a gold mine of information for those interested in using FRET microscopy to study protein-protein interactions. A major strength of this book is that it is focused and yet comprehensive...The book is clearly written, carefully edited, and engaging...It will be a valued resource and practical guide for years to come...5 Stars! (Highest Rating)"--Doody's

Ratings and Reviews