Molecular Genetics Pt 3 - 1st Edition - ISBN: 9780126844030, 9780323151870

Molecular Genetics Pt 3

1st Edition

Editors: Herbert Taylor
eBook ISBN: 9780323151870
Imprint: Academic Press
Published Date: 1st January 1979
Page Count: 406
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Molecular Genetics, Part III: Chromosome Structure explores the structure and modification of DNA, chromatin, and higher order organization and possible subunits of chromosomes at the molecular level. It describes major changes in concepts of chromatin structure and packaging of DNA based on studies of nuclease digests and electron micrographs; the role of restriction endonucleases in molecular genetics; the involvement of DNA topoisomerases in concerted breaking and rejoining of DNA backbone bonds; enzymatic methylation of DNA; and transcriptional units in eukaryotic chromosomes. Organized into seven chapters, this volume begins with an overview of the general properties of type I and type II restriction enzymes, basic aspects of restriction enzyme technology, and applications of restriction enzyme technology to the study of chromosome structure and function. It then discusses recombinant DNA technology; possible biological roles of DNA topoisomerases; recognition and control sequences in nucleic acids; composition and substructure of nucleosomes; analysis of chromosome fibers by electron microscopy; organization of fibers into chromosomes; and functional aspects of organization of chromosome fibers. Molecular biologists, geneticists, scientists, and electron microscopists will find this book extremely helpful.

Table of Contents

Contributors to Part III


Contents of Part I and Part II

Chapter I The Role of Restriction Endonucleases in Molecular Genetics

I. Introduction

II. General Properties of Restriction Enzymes

III. Basic Aspects of Restriction Enzyme Technology

IV. Applications of Restriction Enzyme Technology to the Study of Chromosome Structure and Function

V. Recombinant DNA Technology

VI. Concluding Remarks


Chapter II DNA Topoisomerases: Enzymes That Catalyze the Concerted Breaking and Rejoining of DNA Backbone Bonds

I. Introduction

II. Eco DNA Topoisomerase I

III. Other Prokaryotic DNA Topoisomerases

IV. Eukaryotic DNA Topoisomerases

V. Possible Biological Roles of DNA Topoisomerases

VI. Other Proteins with Properties Similar to DNA Topoisomerases


Chapter III Enzymatic Methylation of DNA: Patterns and Possible Regulatory Roles

I. Introduction

II. Restriction and Modification in Bacterial Cells

III. Sites of Methylation in DNA

IV. Patterns of Methylation of DNA and the Cell Division Cycle

V. Changes in Methylation during Differentiation and Development

VI. Methylating Enzymes Isolated from Cells

VII. Inactivation or Activation of the X Chromosome

VIII. Biological Clocks in Cell Division

IX. Hypothesis on the Control of Replication by Methylation

X. Other Models for Regulatory or Developmental Roles of Methylation



Chapter IV Transcriptional Units in Eukaryotic Chromosomes

I. Introduction

II. Genome Organization and Transcription

III. Multiple-Copy Genes

IV. Single-Copy Genes

V. Perspectives


Chapter V Recognition and Control Sequences in Nucleic Acids

I. Introduction

II. Transcription

III. Regulation of Transcription by Sequence-Specific DNA Binding Proteins

IV. DNA Synthesis

V. Codon Selection in mRNA

VI. A Brief Overview of Other Sequences That Play Roles in Gene Expression

VII. Rapid Nucleic Acid Sequencing Methods


Chapter VI Nucleosomes: Composition and Substructure

I. Introduction

II. Histones

III. Nucleosomes—Discovery, Isolation, and Composition

IV. Nucleosome Structure

V. Concluding Remarks


Chapter VII Nucleosomes and Higher Levels of Chromosomal Organization

I. Introduction

II. The Concept of the Chromosome Fiber

III. Components of Chromosome Fibers

IV. Molecular Organization of Chromosome Fibers

V. Organization of Fibers into Chromosomes

VI. Functional Aspects of Chromosome Fiber Organization




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© Academic Press 1979
Academic Press
eBook ISBN:

About the Editor

Herbert Taylor

Affiliations and Expertise

National Institutes of Health, Bethesda, Maryland, U.S.A.

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