Description

Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first book in which such methodology and molecular biology are combined. The preferred cell lines for genetic analysis are presented in the first section, followed by special techniques for isolating a wide range of mutants. The remainder of the book is devoted to genetic mapping and cloning of mutant genes. The final section presents special techniques in gene regulation.

Readership

Biochemists, geneticists, genetic engineers, microbiologists, molecular biologists, cell biologists, analytical and clinical chemists.

Table of Contents

Cell Lines Useful for Genetic Analysis. M.M. Gottesman, Chinese Hamster Ovary Cells. T. van Daalen Wetters and P. Coffino, Cultured S49 Mouse T Lymphoma Cells. G.J. Darlington, Liver Cell Lines. S.-i. Akiyama, HeLa Cell Lines. D. French, T. Kelly, S. Buhl, and M.D. Scharff, Somatic Cell Genetic Analysis of Myelomas and Hybridomas. H. Jakob and J.-F. Nicolas, Mouse Teratocarcinoma Cells. Special Techniques for Mutant Selection. R.T. Schimke, D.S. Roos, and P.C. Brown, Amplification of Genes in Somatic Mammalian Cells. S. Gal, Replica Plating and Detection of Antigen Variants. M.M. Gottesman, Drug-Resistant Mutants: Selection and Dominance Analysis. D. Patterson and C.A. Waldren, Suicide Selection of Mammalian Cell Mutants. J. Pouyss,gur and A. Franchi, Use of Tritium-Labeled Precursors to Select Mutants. D.J.P. FitzGerald, Construction of Immunotoxins Using Pseudomonas Exotoxin A. J. Hirschberg and M. Marcus, Isolation of Temperature-Sensitive Mutants. M.E. Kamarck, Fluorescence-Activated Cell Sorting of Hybrid and Transfected Cells. Genetic Mapping and Analysis. M.J. Siciliano and B.F. White, Isozyme Identification of Chromosomes in Interspecific Somatic Cell Hybrids. U. Zimmermann and H.B. Urnovitz, Principles of Electrofusion and Electropermeabilization. J.W. Shay, Cell Enucleation, Cybrids, Reconstituted Cells, and Nuclear Hybrids. W.E. Wright, Toxin*b1Antitoxin Selection for Isolating Heterokaryons and Cell Hybrids. M. Bartholdi, J. Meyne, K. Albright, M. Luedemann, E. Campbell, D. Chritton, L.L. Deaven, and L.S. Cram, Chromosome Sorting by Flow Cytometry. J.M. Trent and F.H. Thompson, Methods for Chromosome Banding of Human and Experimental Tumors in Vitro. S.L. Naylor, J.R. McGill, and B.U. Zabel, In Situ Hybridization of Metaphase and Prometaphase Chromosomes. R.V. Lebo and B.D. Bruce, Gene Mapping with Sorted Chromosomes. P.J. Saxon and E.J. Stanbridge, Transfer and Selective Retention of Single Specific Human Chromosomes via Microcell-Mediated Chromosome Tr

Details

No. of pages:
609
Language:
English
Copyright:
© 1987
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080882680
Print ISBN:
9780121820527

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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY