Cell Lines Useful for Genetic Analysis. M.M. Gottesman, Chinese Hamster Ovary Cells. T. van Daalen Wetters and P. Coffino, Cultured S49 Mouse T Lymphoma Cells. G.J. Darlington, Liver Cell Lines. S.-i. Akiyama, HeLa Cell Lines. D. French, T. Kelly, S. Buhl, and M.D. Scharff, Somatic Cell Genetic Analysis of Myelomas and Hybridomas. H. Jakob and J.-F. Nicolas, Mouse Teratocarcinoma Cells. Special Techniques for Mutant Selection. R.T. Schimke, D.S. Roos, and P.C. Brown, Amplification of Genes in Somatic Mammalian Cells. S. Gal, Replica Plating and Detection of Antigen Variants. M.M. Gottesman, Drug-Resistant Mutants: Selection and Dominance Analysis. D. Patterson and C.A. Waldren, Suicide Selection of Mammalian Cell Mutants. J. Pouyss,gur and A. Franchi, Use of Tritium-Labeled Precursors to Select Mutants. D.J.P. FitzGerald, Construction of Immunotoxins Using Pseudomonas Exotoxin A. J. Hirschberg and M. Marcus, Isolation of Temperature-Sensitive Mutants. M.E. Kamarck, Fluorescence-Activated Cell Sorting of Hybrid and Transfected Cells. Genetic Mapping and Analysis. M.J. Siciliano and B.F. White, Isozyme Identification of Chromosomes in Interspecific Somatic Cell Hybrids. U. Zimmermann and H.B. Urnovitz, Principles of Electrofusion and Electropermeabilization. J.W. Shay, Cell Enucleation, Cybrids, Reconstituted Cells, and Nuclear Hybrids. W.E. Wright, Toxin*b1Antitoxin Selection for Isolating Heterokaryons and Cell Hybrids. M. Bartholdi, J. Meyne, K. Albright, M. Luedemann, E. Campbell, D. Chritton, L.L. Deaven, and L.S. Cram, Chromosome Sorting by Flow Cytometry. J.M. Trent and F.H. Thompson, Methods for Chromosome Banding of Human and Experimental Tumors in Vitro. S.L. Naylor, J.R. McGill, and B.U. Zabel, In Situ Hybridization of Metaphase and Prometaphase Chromosomes. R.V. Lebo and B.D. Bruce, Gene Mapping with Sorted Chromosomes. P.J. Saxon and E.J. Stanbridge, Transfer and Selective Retention of Single Specific Human Chromosomes via Microcell-Mediated Chromosome Transfer. Isolation and Detection of Mutant Genes. M.M. Gottesman, Strategies for Isolation of Mutant Genes. I.B. Roninson, Use of in-Gel DNA Renaturation for Detection and Cloning of Amplified Genes. P.-F. Lin, D.B. Brown, P. Murphy, M. Yamaizumi, and F.H. Ruddle, Detection and Characterization of Specific mRNA by Microinjection and Complementation of Mutant Cells. C.M. Fordis and B.H. Howard, Use of the CAT Reporter Gene for Optimization of Gene Transfer into Eukaryotic Cells. M. McCormick, M.E. Gottesman, G.A. Gaitanaris, and B.H. Howard, Cosmid Vector Systems for Genomic DNA Cloning. R. Fleischmann, M. McCormick, and B.H. Howard, Preparation of a Genomic Cosmid Library. B.R. Troen, Colony Screening of Genomic Cosmid Libraries. Y.-F. Lau, Rescue of Genes Transferred from Cosmid Libraries. H. Okayama, Phage-Mediated Transduction of cDNA Expression Libraries into Mammalian Cells. M. McCormick, Sib Selection. L.A. Chasin, A.M. Carothers, and G. Urlaub, Repetitive Cloning of Mutant Genes Using Locus-Specific Sticky Ends. C.L. Smith, S.K. Lawrance, G.A. Gillespie, C.R. Cantor, S.M. Weissman, and F.S. Collins, Strategies for Mapping and Cloning Macroregions of Mammalian Genomes. S.P. Goff, Insertional Mutagenesis to Isolate Genes. J.-E. Edstr"m, R. Kaiser, and D. R"hme, Microcloning of Mammalian Metaphase Chromosomes. Gene Regulation in Tissue Culture. J.T. Stout and C.T. Caskey, Antisense RNA Inhibition of Endogenous Genes. G.H. Smith, In Situ Detection of Transcription in Transfected Cells Using Biotin-Labeled Molecular Probes. M.E. Harper and L.M. Marselle, RNA Detection and Localization in Cells and Tissue Sections by in Situ Hybridization of 35S-Labeled RNA Probes. A. Varshavsky, Electrophoretic Assay for DNA-Binding Proteins. Author Index. Subject Index.
Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first book in which such methodology and molecular biology are combined. The preferred cell lines for genetic analysis are presented in the first section, followed by special techniques for isolating a wide range of mutants. The remainder of the book is devoted to genetic mapping and cloning of mutant genes. The final section presents special techniques in gene regulation.
Biochemists, geneticists, genetic engineers, microbiologists, molecular biologists, cell biologists, analytical and clinical chemists.
- No. of pages:
- © Academic Press 1987
- 11th December 1987
- Academic Press
- eBook ISBN:
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California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA
National Cancer Institute, National Institutes of Health, Bethesda, Maryland, U.S.A.