Description

Computer-based design and modeling, computational approaches, and instrumental methods for elucidating molecular mechanisms of protein folding and ligand-acceptor interactions are included in Volumes 202 and 203, as are genetic and chemical methods for the production of functional molecules including antibodies and antigens, enzymes, receptors, nucleic acids and polysaccharides, and drugs.

Readership

Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.

Table of Contents

Antibodies and Antigens: Antibody Combining Site: E.A. Padlan and E.A. Kabat, Modeling of Antibody Combining Sites. M.B. Bolger and M.A. Sherman, Computer Modeling of the Combining Site Structure of Anti-hapten Monoclonal Antibodies. J.S. Huston, M. Mudgett-Hunter, M.-S. Tai, J. McCartney, F. Warren, E. Haber, and H. Oppermann, Protein Engineering of Single-Chain Fv Analogs and Fusion Proteins. S. Johnson and R.E. Bird, Construction of Single-Chain Fv Derivatives of Monoclonal Antibodies and Their Production in Escherichia coli. D. G~adussow and G. Seemann, Humanization of Monoclonal Antibodies. Antibody-Antigen Interactions: A.C.R. Martin, J.C. ~Cheetham, and A. Rees, Molecular Modeling of Antibody Combining Sites. I.A. Wilson, J.M. Rini, D.H. Fremont, G.G. Fieser, and E.A Stura, X-Ray Crystallographic Analysis of Free and Antigen-Complexed Fab Fragments to Investigate Structural Basis of Immune Recognition. J.L. Pellequer, E. Westhof, and M.H.V. Van Regenmortel, Predicting Location of Continuous Epitopes in Proteins from Their Primary Structures. J.C. Cheetham, C. Redfield, R.E. Griest, C.M. Dobson, and A.R. Rees, Use of Two-Dimensional 1H Nuclear Magnetic Resonance to Study High-Affinity Antibody-Peptide Interactions. J. Anglister and F. Naider, Nuclear Magnetic Resonance for Studying Peptide-Antibody Complexes by Transferred Nuclear Overhauser Effect Difference Spectroscopy. P. Tsang, M. Rance, and P.E. Wright, ~Isotope-Edited Nuclear Magnetic Resonance Studies of Fab-Peptide Complexes. J.E. Jentoft, Reductive Methylation and Carbon-13 Nuclear Magnetic Resonance in Structure-Function Studies of the Fc Fragment and Its Subfragments.

Details

No. of pages:
764
Language:
English
Copyright:
© 1991
Published:
Imprint:
Academic Press
Print ISBN:
9780121821043
Electronic ISBN:
9780080883182

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY