Molecular Biology

Molecular Biology

A Project Approach

1st Edition - November 15, 1995

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  • Author: Susan Karcher
  • eBook ISBN: 9780080536835

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This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.

Key Features

  • Includes a glossary of over 200 terms important for understanding molecular biology
  • Uses an inexpensive source of eukaryotic cells - great for schools on a budget
  • Includes Methods Locator that provides instant access to the latest methods
  • Contain clearly written, easy-to-follow, student-tested instructions:
    • Sterile techniques
    • Phage titration
    • Gel electrophoresis of DNA
    • Restriction enzyme digestion
    • Plasmid isolation
    • Transformation of E. Coli
    • Recombinant DNA cloning
    • Nick translation labeling
    • Nonradioactive primer labelling
    • Nonradioactive DNA detection
    • Southern blotting
    • Colony hybridization
    • Purification of plant DNA
    • RNA purification
    • Northern blotting
    • Purification of poly A+ RNA
    • Polymerase chain reaction (PCR)


Juniors, seniors, and beginning graduate students in molecular biology and genetics will be interested

Table of Contents

  • Transposon Mutagenesis of Escherichia coli.
    Recombinant DNA and Cloning.
    Southern Blot Analysis.
    Plant Genomic Southern Blotting with Probes for Low- and High-Copy-Number Genes.
    RNA Purification and Northern Blot Analysis.
    Polymerase Chain Reaction.

Product details

  • No. of pages: 280
  • Language: English
  • Copyright: © Academic Press 1995
  • Published: November 15, 1995
  • Imprint: Academic Press
  • eBook ISBN: 9780080536835

About the Author

Susan Karcher

Affiliations and Expertise

Purdue University, West Lafayette, Indiana, U.S.A.

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