Molecular Biology of Plant Nuclear Genes - 1st Edition - ISBN: 9780127150062, 9780323147323

Molecular Biology of Plant Nuclear Genes

1st Edition

Editors: Indra Vasil
eBook ISBN: 9780323147323
Imprint: Academic Press
Published Date: 28th July 1989
Page Count: 520
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Cell Culture and Somatic Cell Genetics of Plants, Volume 6: Molecular Biology of Plant Nuclear Genes focuses on the spectacular and rapid advances in the molecular biology and genetics of plants.

This book consists of 19 chapters. Chapters 1 to 5 describe the most commonly used approaches for the genetic transformation of plants. The light-inducible and tissue-organ-specific genes are discussed in Chapters 6 to 11. In Chapters 12 to 14, the genes regulating phytohormone synthesis, heat shock proteins, and nodulation in legume roots are treated, while in Chapters 15 to 16, the relationship between chromatin structure and gene expression and molecular biology of plant RNA viruses are analyzed. The development of transgenic plants resistant to viruses, insects, and herbicides is dealt with in the last three chapters.

This volume is suitable for plant molecular biologist, genetic engineers, and researchers concerned with plant cell and tissue culture.

Table of Contents


General Preface


Contents of Previous Volumes

1 Plant Gene Vectors and Genetic Transformation: Plant Transformation Systems Based on the Use of Agrobacterium tumefaciens

I. Transformation of Plants by Agrobacterium tumefaciens

II. Agrobacterium Hosts

III. Basic Transformation Vectors

IV. Selectable Markers

V. Miscellaneous Features

VI. Specialized Vectors

VII. Conclusions


2 Plant Gene Vectors and Genetic Transformation: Agrobacterium Ri Plasmids

I. Introduction

II. Relevant Features of Hairy Root with Respect to Plant Transformation

III. Ri Plasmid-Based DNA Transfer Strategies

IV. Selection of Transformants

V. Conclusion


3 Plant Gene Vectors and Genetic Transformation: DNA-Mediated Direct Gene Transfer to Plants

I. General Introduction

II. Direct Gene Transfer System

III. Foreign DNA in Transgenic Plants

IV. Evaluation of Direct Gene Transfer

V. Experimental Applications of Direct Gene Transfer

VI. Conclusions


4 Plant Gene Vectors and Genetic Transformation: Plant Viruses as Vectors

I. Introduction

II. CaMV and Its Development into a Plant Vector

III. CaMV as a Tool in Plant Genetic Engineering

IV. Geminiviruses

V. Vectors Based on RNA Plant Viruses

VI. Conclusion and Outlook


5 Plant Gene Vectors and Genetic Transformation: The Structure, Function, and Uses of Maize Transposable Elements

I. Introduction

II. The Structure of Maize Transposable Elements

III. The Function and Regulation of Spm and Ac

IV. Using Maize Transposable Elements


6 Structure and Regulation of Light-Inducible Genes: Genes Involved in Photosynthesis

I. Introduction

II. Gene Structure and Linkage

III. Expression Dynamics

IV. Cis-Acting Elements for Light-Regulated Transcription

V. Trans-Acting Factors for Light-Regulated Transcription

VI. Conclusions and Prospects


7 Regulation and Structure of Chalcone Synthase Genes

I. Introduction

II. Modes of CHS Regulation

III. Structure, Organization, and Specific Stimulation of CHS Genes

IV. Regulatory Elements of the CHS Promoter

V. Perspectives


8 Structure and Regulation of Light-inducible Genes: Phytochrome, Properties of a Photoreceptor That Regulates Its Own Expression

I. Introduction

II. Gene Characterization

III. Regulation of Phytochrome Expression

IV. Regulation of Gene Expression by Phytochrome

V. Other Forms of Phytochrome

VI. Conclusion


9 Structure and Regulation of Organ- and Tissue-Specific Genes: Regulated Expression of Flower-Specific Genes

I. Introduction

II. Isolation of Flower-Specific Genes

III. Characterization of Flower-Specific Genes

IV. Cellular- and Tissue-Specific Expression Patterns

V. Discussion


10 Structure and Regulation of Organ- and Tissue-Specific Genes in Plants

I. Introduction

II. Plant Chitinase

III. Induction of Chitinase Activity

IV. Plant Chitinase Genes

V. Bacterial Chitinases

VI. Bacterial Chitinase Genes

VII. Expression of a Bacterial Chitinase Gene in Plant Cells

VIII. Activity of a Bacterial Chitinase Gene in Plants

IX. Conclusions


11 Structure and Regulation of Organ- and Tissue-Specific Genes: Structural and Cytological Features of Incompatibility Gene Expression in Flowering Plants

I. Introduction

II. Homomorphic Systems

III. The Heteromorphic System

IV. Interspecific Incompatibility

V. Nature of the S-Gene Products

VI. Concluding Comments


12 Phytohormone Synthesis: Pathways, Genes, and Mutations

I. Introduction

II. Growth Hormone Synthesis: Pathways, Genes, and Mutations

III. Concluding Remarks


13 Heat Shock Protein Genes of Plants

I. Introduction

II. Regulation of Heat Shock Gene Expression

III. Heat Shock Proteins

IV. Heat Shock Protein Structure

V. Heat Shock Protein Gene Structure

VI. Concluding Remarks


14 Nodule Development and Formation of the Endosymbiotic Compartment

I. Introduction

II. Cell Commitment in Nodule Development

III. Plant-Microbe Signaling

IV. The Peribacteroid Compartment: A Nitrogen-Fixing Organelle

V. Carbon and Nitrogen: Altered Metabolic Pathways


15 Chromatin Structure and Gene Expression

I. Introduction

II. First-Order Structure and Gene Regulation

III. Proteins That Interact with DNA in Hypersensitive Regions


16 Molecular Biology of Plant RNA Viruses

I. Introduction

II. Viruses with a Capped RNA Genome

III. Viruses with a 5' Terminal VPg at Their RNA Genome

IV. Concluding Remarks


17 Genetic Engineering of Plants for Viral Disease Resistance

I. Introduction

II. Virus Resistance in Plants Expressing Viral CP Genes

III. Resistance Conferred by Expression of CP Antisense Transcripts

IV. Protection Engineered by Expression of Viral Satellite RNA

V. Expression of Other Viral Genes Important to Virus Infection

VI. Conclusions


18 Protein Engineering in Plants: Expression of Bacillus thuringiensis Insecticidal Protein Genes

I. Introduction

II. Structural and Functional Properties of a Lepidoptera-Specific ICP

III. Expression of Toxin Genes in Plants

IV. Selection of B. thuringiensis Genes


19 Selective Herbicide Tolerance through Protein Engineering

I. Introduction

II. Glyphosate Tolerance

III. Sulfonylurea Herbicide Tolerance

IV. Imidazolinone Herbicide Tolerance

V. Phosphinothricin Tolerance

VI. Bromoxynil Tolerance

VII. Triazine Herbicide Tolerance

VIII. Conclusion and Future Prospects




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© Academic Press 1989
Academic Press
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About the Editor

Indra Vasil

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