Molecular Approaches to Neurobiology

Molecular Approaches to Neurobiology

1st Edition - January 28, 1982

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  • Editor: Ian R. Brown
  • eBook ISBN: 9781483282299

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Molecular Approaches to Neurobiology deals with molecular approaches to the analysis of the nervous system. This book surveys the current state of knowledge in a number of areas of molecular neurobiology that includes the subcellular level of chromatin structure, complexity of RNA synthesis, role of hormones in cell differentiation, and molecular correlates between neuropeptides and behavior. A comprehensive review of procedures for the isolation of specific brain cells and their experimental use is also provided at the end of this text. This publication is useful to those working in the field of neurochemistry and those engaged in morphological and physiological approaches to the analysis of the nervous system, as well as molecular biologists and biochemists working with non-neural tissues.

Table of Contents

  • List of Contributors


    1 Current Genetic Approaches to the Mammalian Nervous System

    I. Introduction

    II. Inherited Variations in Genes and Gene Products

    III. Generating Genetic Variants

    IV. Assessing the Role of Heredity in Behavior and Disease

    V. Conclusion


    2 Chromosomal Components in Brain Cells

    I. Introduction

    II. Organization of Chromatin

    III. Brain Histones

    IV. Nonhistone Chromosomal Proteins (NHCP) of the Brain

    V. DNA Polymerase

    VI. RNA Polymerase

    VII. Concluding Remarks


    3 The Sequence Complexity of Brain Ribonucleic Acids

    I. Introduction

    II. RNA-DNA Hybridization Analysis

    III. Complexity of Gene Transcription in the Brain

    IV. RNA Sequence Complexity at the Level of Translation

    V. Regional and Cellular Distribution of Gene Transcripts

    VI. Gene Activity during Development and Aging

    VII. Concluding Remarks


    4 Molecular Characterization of Synapses of the Central Nervous System

    I. Introduction

    II. Isolation and Characterization of Synaptic Junctional Complexes and Postsynaptic Densities

    III. Protein Composition of Isolated Synaptic Junctions and Postsynaptic Densities

    IV. Functional Properties of Synaptic Junctions and Postsynaptic Densities

    V. Development of the Synapse

    VI. Conclusions


    5 Axonal Transport of Macromolecules

    I. Introduction

    II. Slow Axonal Transport

    III. Rapid Axonal Transport

    IV. Conclusions


    6 Mechanistic Studies on the Cellular Effects of Nerve Growth Factor

    I. NGF and Its Target Cells

    II. Role of Transcription in NGF Mechanism of Action on Neurite Outgrowth

    III. Priming Model of Neurite Outgrowth

    IV. Transcriptional Regulation of NGF Responses Other Than Neurite Outgrowth

    V. NGF Receptors and Transcription

    VI. Nontranscriptional, Anabolic, and Rapid Effects of NGF

    VII. Genetic Approaches to NGF Molecular Mechanism

    VIII. NGF and Cell Proliferation

    IX. Concluding Remarks


    7 Cell Interactions in Embryonic Neural Retina: Role in Hormonal Induction of Glutamine Synthetase

    I. Introduction

    II. Neural Retina of Chick Embryo

    III. Glutamine Synthetase (GS) in Avian Neural Retina

    IV. Induction of GS

    V. Cell Interactions and GS Induction

    VI. Cortisol Receptors

    VII. Comment


    8 Sexual Differentiation of the Brain: Gonadal Hormone Action and Current Concepts of Neuronal Differentiation

    I. Introduction

    II. General Plan of Sexual Differentiation for Mammals

    III. Sexual Differentiation in Rats

    IV. Morphological Sex Differences in the Brain

    V. Sexual Differentiation in Relation to Current Concepts of Neuronal Development and Differentiation

    VI. Conclusions


    9 Analysis of Protein Synthesis in the Mammalian Brain Using LSD And Hyperthermia as Experimental Probes

    I. Introduction

    II. Perturbation of Protein Synthesis in the Brain

    III. Effect of LSD on the Translational Apparatus of the Brain

    IV. Effect of Hyperthermia on the Translational Apparatus of the Brain

    V. Effect of LSD and Hyperthermia on Brain Protein Synthesis In Vivo

    VI. Effect of LSD and Hyperthermia on Subsequent Cell-Free Protein Synthesis in the Brain

    VII. Concluding Remarks


    10 Neuropeptides as Putative Neurotransmitters: Endorphins, Substance P, Cholecystokinin, and Vasoactive Intestinal Polypeptide

    I. Introduction

    II. Endogenous Opioid Peptides

    III. Substance P

    IV. Cholecystokinin and Vasoactive Intestinal Polypeptide

    V. Conclusion


    11 Molecular Correlates between Pituary Hormones and Behavior

    I. Introduction

    II. Behavioral Effects of Pituitary Hormones

    III. Molecular Correlates of Pituitary Peptide Hormones

    IV. Concluding Remarks


    12 Macromolecules and Behavior

    I. Introduction

    II. General Approaches to the Relationships between Molecules and Behavior

    III. Deoxyglycose Procedure

    IV. Learning and Memory Studies

    V. Neuropeptides as Tools for Studying the Biochemistry of Behavior

    VI. Receptors

    VII. Concluding Remarks


    13 Isolation and Culture of Specific Brain Cells and Their Experimental Use

    I. Introduction

    II. General Approaches to the Isolation of Brain Cells

    III. Microscale Isolation Methods

    IV. Macroscale Isolation Methods

    V. Biochemical Properties of Neuronal and Glial Cells Isolated from "Normal" Brain Tissue

    VI. Biochemical Properties of Neural Cells Isolated from Pathologically Altered Brain Tissue

    VII. Culturing of Isolated Neurons and Glia from Brains of Young Adult Animals—A Preliminary Survey

    VIII. Summary and Outlook



Product details

  • No. of pages: 434
  • Language: English
  • Copyright: © Academic Press 1982
  • Published: January 28, 1982
  • Imprint: Academic Press
  • eBook ISBN: 9781483282299

About the Editor

Ian R. Brown

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