Modern Methods in the Analysis and Structural Elucidation of Mycotoxins

Modern Methods in the Analysis and Structural Elucidation of Mycotoxins

1st Edition - October 28, 1986

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  • Editor: Richard J. Cole
  • eBook ISBN: 9780323148535

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Modern Methods in the Analysis and Structural Elucidation of Mycotoxins presents available methods of analysis and structural elucidation of mycotoxins by recognized experts in the various disciplines. The approach in each chapter of the book is to present each method initially in theoretical terms and then to review the method as it specifically applies to the analysis and/or structural elucidation of mycotoxins. Comprised of 15 chapters, the book's opening chapters deal with screening, sampling, and survey methods for mycotoxins and toxigenic fungi. This is followed by chapters dealing mostly with methods for structural elucidation, such as NMR and X-ray crystallography and IR and UV spectroscopy, as well as biosynthetic techniques. Significant chapters consider the analytical methods for mycotoxin analyses, including enzyme-linked immunosorbent assay system and tandem mass spectrometry. The concluding chapter examines the mycotoxin analytical problem in taxonomic or ecological terms. This book is of value to food and feed researchers, scientists, and manufacturers who are interested in product contamination control.

Table of Contents

  • Preface

    1 Biological Screening Methods for Mycotoxins and Toxigenic Fungi

    I. Introduction

    II. Animal Assays

    III. Plant Assays

    IV. Microbiological Assays

    V. Conclusion


    2 Sampling and Sample Preparation Methods for Mycotoxin Analysis

    I. Introduction

    II. Distribution of Mycotoxins within the Product

    III. Sources of Error in Mycotoxin Tests

    IV. Error Reduction

    V. Sampling Procedures

    VI. Subsampling Procedures

    VII. Preservation of Samples

    VIII. Survey Studies

    IX. Quality Control

    X. Experimentation


    3 Chemical Survey Methods for Mycotoxins

    I. Introduction

    II. Aflatoxins

    III. Zearalenone

    IV. Trichothecenes

    V. Ochratoxins

    VI. Sterigmatocystin

    VII. Conclusion and Future Developments


    4 Application of Ultraviolet and Infrared Spectroscopy in the Analysis and Structural Elucidation of Mycotoxins

    I. Introduction

    II. Infrared Spectroscopy

    III. Ultraviolet Spectroscopy

    IV. Conclusion


    5 Application of Nuclear Magnetic Resonance to Structural Elucidation of Mycotoxins

    I. Introduction

    II. Strategies in Structural Determination

    III. One-Dimensional Methods

    IV. Spectral Editing in 13C NMR Spectra

    V. Two-Dimensional Methods

    VI. Carbon-Carbon Connectivity

    VII. Biosynthetic Studies


    6 X-Ray Crystallography as a Tool for Identifying New Mycotoxins

    I. Introduction

    II. History and Description of the Single-Crystal X-Ray Diffraction Experiment

    III. Advantages and Limitations of Using X-Ray Diffraction

    IV. X-Ray Diffraction of Mycotoxins

    V. Conclusions


    7 Application of Biosynthetic Techniques in the Structural Studies of Mycotoxins

    I. Introduction

    II. Techniques

    III. Biosynthesis of Polyketides

    IV. Amino Acids in the Biosynthesis of Mycotoxins


    8 Immunoassays for Mycotoxins

    I. Introduction

    II. Production of Antibody against Mycotoxins

    III. Characterization of Antibody

    IV. Radioimmunoassay for Mycotoxins

    V. Enzyme-Linked Immunosorbent Assay for Mycotoxins

    VI. Immunohistochemical Methods and Other Studies

    VII. Concluding Remarks


    9 Thin-Layer Chromatography/High-Performance Thin-Layer Chromatography as a Tool for Mycotoxin Determination

    I. Introduction

    II. Thin-Layer Chromatographic Procedures for Mycotoxins

    III. Analysis of Individual Mycotoxins

    IV. Conclusions


    10 Gas Chromatography in Mycotoxin Analysis

    I. Introduction

    II. Practical Aspects of Gas Chromatography

    III. Use of Gas Chromatography in the Analysis of Mycotoxins


    11 High-Performance Liquid Chromatography and Its Application to the Analysis of Mycotoxins

    I. Introduction

    II. High-Performance Liquid Chromatographic Instrumentation and Methodology

    III. Manipulation of High-Performance Liquid Chromatographic Conditions

    IV. High-Performance Liquid Chromatography: A Literature Review

    V. Conclusions


    12 Gas Chromatographic-Mass Spectrometric Analysis of Mycotoxins

    I. Introduction

    II. History and Instrumentation

    III. Comparison of Mass Spectrometry Techniques

    IV. Quantitative Analysis

    V. Mycotoxins

    VI. Conclusions


    13 Mass Spectra of Selected Trichothecenes

    I. Introduction

    II. Application to Selected Trichothecene Mycotoxins

    III. Analysis of a Mixture of Trichothecenes by Gas Chromatography and Identification of Some of the Components by Searching a Computer Library of Positive Chemical Ionization Spectra

    IV. Conclusions


    14 Mass Spectrometry-Mass Spectrometry as a Tool for Mycotoxin Analysis

    I. Introduction

    II. Mass Spectrometry-Mass Spectrometry Instrumentation

    III. Mass Spectrometry-Mass Spectrometry Techniques and Experiments

    IV. Examples of Mass Spectrometry-Mass Spectrometry of Mycotoxins


    15 Taxonomic Approaches to Mycotoxin Identification (Taxonomic Indication of Mycotoxin Content in Foods)

    I. Introduction

    II. Factors Affecting Mycotoxin Production

    III. Mycological Examination of Foods

    IV. Mycotoxins and Fungal Taxonomy

    V. Important Genera Containing Toxigenic Isolates



Product details

  • No. of pages: 486
  • Language: English
  • Copyright: © Academic Press 1986
  • Published: October 28, 1986
  • Imprint: Academic Press
  • eBook ISBN: 9780323148535

About the Editor

Richard J. Cole

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