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Modern Methods in the Analysis and Structural Elucidation of Mycotoxins - 1st Edition - ISBN: 9780121795153, 9780323148535

Modern Methods in the Analysis and Structural Elucidation of Mycotoxins

1st Edition

Editor: Richard J. Cole
eBook ISBN: 9780323148535
Imprint: Academic Press
Published Date: 28th October 1986
Page Count: 486
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Modern Methods in the Analysis and Structural Elucidation of Mycotoxins presents available methods of analysis and structural elucidation of mycotoxins by recognized experts in the various disciplines. The approach in each chapter of the book is to present each method initially in theoretical terms and then to review the method as it specifically applies to the analysis and/or structural elucidation of mycotoxins. Comprised of 15 chapters, the book's opening chapters deal with screening, sampling, and survey methods for mycotoxins and toxigenic fungi. This is followed by chapters dealing mostly with methods for structural elucidation, such as NMR and X-ray crystallography and IR and UV spectroscopy, as well as biosynthetic techniques. Significant chapters consider the analytical methods for mycotoxin analyses, including enzyme-linked immunosorbent assay system and tandem mass spectrometry. The concluding chapter examines the mycotoxin analytical problem in taxonomic or ecological terms. This book is of value to food and feed researchers, scientists, and manufacturers who are interested in product contamination control.

Table of Contents


1 Biological Screening Methods for Mycotoxins and Toxigenic Fungi

I. Introduction

II. Animal Assays

III. Plant Assays

IV. Microbiological Assays

V. Conclusion


2 Sampling and Sample Preparation Methods for Mycotoxin Analysis

I. Introduction

II. Distribution of Mycotoxins within the Product

III. Sources of Error in Mycotoxin Tests

IV. Error Reduction

V. Sampling Procedures

VI. Subsampling Procedures

VII. Preservation of Samples

VIII. Survey Studies

IX. Quality Control

X. Experimentation


3 Chemical Survey Methods for Mycotoxins

I. Introduction

II. Aflatoxins

III. Zearalenone

IV. Trichothecenes

V. Ochratoxins

VI. Sterigmatocystin

VII. Conclusion and Future Developments


4 Application of Ultraviolet and Infrared Spectroscopy in the Analysis and Structural Elucidation of Mycotoxins

I. Introduction

II. Infrared Spectroscopy

III. Ultraviolet Spectroscopy

IV. Conclusion


5 Application of Nuclear Magnetic Resonance to Structural Elucidation of Mycotoxins

I. Introduction

II. Strategies in Structural Determination

III. One-Dimensional Methods

IV. Spectral Editing in 13C NMR Spectra

V. Two-Dimensional Methods

VI. Carbon-Carbon Connectivity

VII. Biosynthetic Studies


6 X-Ray Crystallography as a Tool for Identifying New Mycotoxins

I. Introduction

II. History and Description of the Single-Crystal X-Ray Diffraction Experiment

III. Advantages and Limitations of Using X-Ray Diffraction

IV. X-Ray Diffraction of Mycotoxins

V. Conclusions


7 Application of Biosynthetic Techniques in the Structural Studies of Mycotoxins

I. Introduction

II. Techniques

III. Biosynthesis of Polyketides

IV. Amino Acids in the Biosynthesis of Mycotoxins


8 Immunoassays for Mycotoxins

I. Introduction

II. Production of Antibody against Mycotoxins

III. Characterization of Antibody

IV. Radioimmunoassay for Mycotoxins

V. Enzyme-Linked Immunosorbent Assay for Mycotoxins

VI. Immunohistochemical Methods and Other Studies

VII. Concluding Remarks


9 Thin-Layer Chromatography/High-Performance Thin-Layer Chromatography as a Tool for Mycotoxin Determination

I. Introduction

II. Thin-Layer Chromatographic Procedures for Mycotoxins

III. Analysis of Individual Mycotoxins

IV. Conclusions


10 Gas Chromatography in Mycotoxin Analysis

I. Introduction

II. Practical Aspects of Gas Chromatography

III. Use of Gas Chromatography in the Analysis of Mycotoxins


11 High-Performance Liquid Chromatography and Its Application to the Analysis of Mycotoxins

I. Introduction

II. High-Performance Liquid Chromatographic Instrumentation and Methodology

III. Manipulation of High-Performance Liquid Chromatographic Conditions

IV. High-Performance Liquid Chromatography: A Literature Review

V. Conclusions


12 Gas Chromatographic-Mass Spectrometric Analysis of Mycotoxins

I. Introduction

II. History and Instrumentation

III. Comparison of Mass Spectrometry Techniques

IV. Quantitative Analysis

V. Mycotoxins

VI. Conclusions


13 Mass Spectra of Selected Trichothecenes

I. Introduction

II. Application to Selected Trichothecene Mycotoxins

III. Analysis of a Mixture of Trichothecenes by Gas Chromatography and Identification of Some of the Components by Searching a Computer Library of Positive Chemical Ionization Spectra

IV. Conclusions


14 Mass Spectrometry-Mass Spectrometry as a Tool for Mycotoxin Analysis

I. Introduction

II. Mass Spectrometry-Mass Spectrometry Instrumentation

III. Mass Spectrometry-Mass Spectrometry Techniques and Experiments

IV. Examples of Mass Spectrometry-Mass Spectrometry of Mycotoxins


15 Taxonomic Approaches to Mycotoxin Identification (Taxonomic Indication of Mycotoxin Content in Foods)

I. Introduction

II. Factors Affecting Mycotoxin Production

III. Mycological Examination of Foods

IV. Mycotoxins and Fungal Taxonomy

V. Important Genera Containing Toxigenic Isolates




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© Academic Press 1986
28th October 1986
Academic Press
eBook ISBN:

About the Editor

Richard J. Cole

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