Modern Approaches to Animal Cell Technology - 1st Edition - ISBN: 9780408027328, 9781483145099

Modern Approaches to Animal Cell Technology

1st Edition

Editors: R.E. Spier J.B. Griffiths
eBook ISBN: 9781483145099
Imprint: Butterworth-Heinemann
Published Date: 1st January 1987
Page Count: 846
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Modern Approaches to Animal Cell Technology is a collection of papers presented at the 1987 joint European Society for Animal Cell Technology-OHOLO conference held in Tiberias, Israel. Contributors explore modern approaches to animal cell technology and discuss the construction of the animal cell substrate, the physiology of those cells in a bioreactor type of environment, and the ways in which different products can be made from animal cells in culture and tested.
This book is comprised of 59 chapters divided into nine sections and begins by outlining the history of issues and decisions that were made regarding the acceptability of various cell substrates, along with the use of continuous cell lines in biotechnology. The next chapter explores the feasibility, reproducibility, and the sensitivity of the dot-blot filter hybridization test to detect minute amounts of residual cellular DNA. The reader is then introduced to cells and cell lines such as monoclonal antibodies; cell growth and development; physiology of cells; and the use of bioreactors for culturing animal cells. Downstream unit processes, vaccines, immune system products, and toxicity testing with animal cells are also considered. This monograph will be a valuable resource for animal-cell technologists, biotechnologists, and microbiologists.

Table of Contents

1. The Liberation of Animal Cells: Psychology of Changing Attitudes

2. Cellular DNA Biologicals: Significance and Quantitation

Section 1 New Cells and Cell Lines

3. Human Monoclonal Antibodies - Their Potential, Problems and Prospects

4. Production of Cancer Specific Monoclonal aAtibodies with Human-Human Hybridomas and Their Serum-free, High density, Perfusion Culture

5. Establishment of a Human Hybridoma Secreting Monoclonal Antibodies toward Rabies Virus

6. The Production of Bovine and Ovine Monoclonal Antibodies to Gonadal Steroids: The Use of Interspecific Hybridomas

7. The Immortalization of Differentiated Mammalian Cells

8. Structural Basis of Growth Factor Receptor Mediated Cellular Signals (Abstract)

9. Growth Control of Normal Human B Lymphocytes

10. Anti-proliferative Effects and Phenotypic Alterations Induced by Methylurea Derivatives in B16 Mouse Melanoma

11. Comparative Analysis of Major Biological Aspects of Human Hepatoma in Culture

12. Mini-review: Essential Strategies for the Application of Herpes Simplex Virus as a Vector for the Expression of Foreign Genes

13. The Glutamine Synthetase Gene: A Novel Marker for Gene Amplification in Mammalian Cells (Abstract)

14. Recombinant Animal Cell Lines for Production of Glycoproteins

15. Foreign Gene Expression in Rat Pituitary GH3 Cells

16. Use of Transfected and Amplified Drosophila Heat Shock Promoter Construction for Inducible Production of Toxic Mouse c-myc Proteins in CHO Cells

Section 2 Medium Development

17. The Use of Glutamine-free Media for the Growth of Three Cell Lines in Microcarrier Culture

18. The Attachment of MDCK Cells to Three Types of Microcarriers in Different Serum-free Media

19. Spreading, Growth and Function of Cells Attached to a Plasma Clot Interphase between Agarose and the Atmosphere

Section 3 Physiological Investigations

20. Investigations of the Influence of Physical Environment on the Cultivation of Animal Cells

21. Shear Sensitivity of Three Hybridoma Cell Lines in Suspension Culture

22. NMR Metabolic Studies of Human Breast Cancer Cells

23. Optimization of Glass-sphere Immobilized Bed Cultures

24. Examination of a Simple Model for the Diffusion of Oxygen into Dense Masses of Animal Cells

25. Kinetics of Prourokinase Production by Mammalian Cells in Culture

26. Specific Kinetic Patterns of Production of Monoclonal Antibodies in Batch Cultures and Consequences for Fermentation Processes

27. A Model System to Study Gene Expression and Scale up of Microcarrier Cultures of Recombinant DNA Manipulated Cells

Section 4 Bioreactor Developments

28. Membrane-Stirrer-Reactor for Bubble Free Aeration and Perfusion

29. Immobilization of Hybridomas in Packed-bed Bioreactors

30. Production of Biologicals by Animal Cells Immobilized in Polyurethane Foam Matrix

31. Comparison of Oxygenation Methods in a 39L Stirred Bioreactor

32. Development of Large-scale Mammalian Cell Perfusion Culture Systems

33. Microcarrier DORMACELL does not Detectably Alter Viral or Plasmid DNA upon Direct Exposure

34. Macroporous Microcarriers

35. Growth of Animal Cells on Microbeads III: Semi Continuous in Situ Estimation of Numbers

36. Evaluation of the Continuous Perfusion Culture System for the Production of Monoclonal Antibodies

37. Recent Advances in the Control of Monitoring of Bioreactors

38. Automated Process Control for Culturing Animal Cells

Section 5 Downstream Unit Processes

39. Design Criteria for the Purification of Biopolymers for the Pharmaceutical Industry

40. Use of a Dynamic Filtration Method for Separation of Animal Cells

41. Continuous Culture of Murine Hybridomas with Integrated Recovery of Monoclonal Antibodies

Section 6 Vaccine Products

42. Improved Production of Marek's Disease Vaccine with the Multi-tube Technology

43. Procedure for the Adoption of the New Mumps Vaccine Strain 'Rubini' to Human Diploid Cells

44. Immuno Detection of Flavi Viruses by Monoclonal Antibodies Directed against West Nile Virus Specific and Flavivirus Group Epitopes

45. A Simple ELISA for the Estimation of Antibodies to Equine Herpes Virus (EHV-1) and Its Application to the Quality Control of Vaccines

46. The Production and Application of an Oil Adjuvant Vaccine against Foot-and-Mouth Disease in Cattle

Section 7 Immune System Products

47. Application of Large Scale Perfusion Systems for Pharmaceutical Production from Mammalian Cells

48. The Organotype Culture Model of Human Cancer Cells: The Effect of Human Recombinant Interferons

49. Immune Interferon Enhances the Expression of MHC Antigens in Human Placenta

50. Gamma Interferon and Anti-thy-l Antibody Induce Specific Killing Activity in Memory-like Cytolytic Hybridomas

51. Development of a Device and a Double Antibody Microradioimmune Assay for Fast Screening and Sensitive Quantitation of Monoclonal Antibodies

52. Development of Monoclonal Antibodies Directed against Carcinoembryonic Antigen (CEA) and Their Application for CEA Detection

53. Carcinoembryonic Antigen (CEA) Production in Low Protein Growth Medium

54. Production and Purification of a Bladder Cancer Associated Membrane Protein

Section 8 Toxicity Testing with Animal Cells

55. The Use of Animal Cells for Evaluation of Toxicity, Carcinogenesis and Mutagenesis

56. Development of a Model for the Study of Toxicity and Carcinogenicity In Vitro 000

Section 9 A Glimpse of the Future

57. The Transformation of Science Fiction into Technical Reality by Using Animal Cells in Culture


58. Terms and Symbols for Use in Animal Cell Culture

Late Paper

59. Hybridoma Culture for the Continuous Production of Monoclonal Antibodies; a Fractionalized Factorial Design Experiment for Process Optimization I

List of Participants

List of Exhibitors


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About the Editor

R.E. Spier

J.B. Griffiths

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