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Helder Maiato and Melina Schuh
1. Assays for the spindle assembly checkpoint in cell culture
Chiara Marcozzi and Jonathon Pines
2. Quantitative methods to measure aneuploidy and chromosomal instability
Kristina M. Godek and Duane A. Compton
3. Dissecting the role of the tubulin code in mitosis
Luísa T. Ferreira, Ana C. Figueiredo, Bernardo Orr, Danilo Lopes and Helder Maiato
4. Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis
Kara L. McKinley
5. Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells
Bramwell G. Lambrus, Tyler C. Moyer and Andrew J. Holland
6. Small molecule tools in mitosis research
Franziska Teusel, Lars Henschke and Thomas U. Mayer
7. Optogenetic control of mitosis with photocaged chemical dimerizers
Huaiying Zhang, David M. Chenoweth and Michael A. Lampson
8. Measuring mitotic forces
Anna A. Ye and Thomas J. Maresca
9. Employing the one-cell C. elegans embryo to study cell division processes
Neil Hattersley, Pablo Lara-Gonzalez, Dhanya Cheerambathur, Sebastian Gomez, Taekyung Kim, Bram Prevo, Renat Khaliullin, Kian-Yong Lee, Midori Ota, Rebecca Green, Karen Oegema and Arshad Desai
10. A cell-free system of Drosophila egg explants supporting native mitotic cycles
Jorge de-Carvalho, Ojas Deshpande, Catarina Nabais and Ivo A. Telley
11. Living Xenopus oocytes, eggs, and embryos as models for cell division
Ani Varjabedian, Angela Kita and William Bement
12. Spreading of chromosomes from mouse oocytes and mammalian cultured cells for light microscopic analysis
Mariana C.C. Silva, Gordana Wutz, Kikuë Tachibana and Jan-Michael Peters
13. In vitro reconstitution of lateral to end-on conversion of kinetochore–microtubule attachments
Manas Chakraborty, Ekaterina Tarasovetc and Ekaterina L. Grishchuk
14. Isolation of mitotic chromosomes from vertebrate cells and characterization of their proteome by mass spectrometry
Itaru Samejima and William C. Earnshaw
15. Purification of kinetochores from the budding yeast Saccharomyces cerevisiae
Amitabha Gupta, Rena K. Evans, Lori B. Koch, Aimee J. Littleton and Sue Biggins
16. Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae
Rokas Grigaitis, Aitor Susperregui, Philipp Wild and Joao Matos
17. Single-nucleus Hi-C of mammalian oocytes and zygotes
Johanna Gassler, Ilya M. Flyamer and Kikuë Tachibana
18. Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells
Hidetaka Miyauchi, Hiroshi Ohta and Mitinori Saitou
19. Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos
Greg FitzHarris, John Carroll and Karl Swann
20. Single cell genomics to study DNA and chromosome changes in human gametes and embryos
Robert C. Blanshard, Chongyi Chen, X. Sunney Xie and Eva R. Hoffmann
21. Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes
Hirohisa Kyogoku, Shuhei Yoshida and Tomoya S. Kitajima
Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Analyzing the Spindle Assembly Checkpoint in human cell culture, an Analysis of CIN, a Functional analysis of the tubulin code in mitosis, Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis, Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells, Small Molecule Tools in Mitosis Research, Optogenetic control of mitosis with photocaged chemical, and more.
- Contains contributions from experts in the field from across the world
- Covers a wide array of topics on both mitosis and meiosis
- Includes relevant, analysis based topics
Researchers and professionals in Cell Biology field
- No. of pages:
- © Academic Press 2018
- 24th May 2018
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Helder Maiato graduated in Biochemistry and holds a PhD in Biomedical Sciences from the University of Porto. He was a visiting PhD student at the University of Edinburgh (UK) and a Post-doctoral Research Affiliate at the New York State Department of Health (USA). At present, he is a Principal Investigator at the Institute for Research and Innovation in Health (i3S) and Invited Professor at the Faculty of Medicine of the University of Porto (Portugal). In 2015 he was honoured with the Young Investigator Award from the Louis-Jeantet Foundation and was recently elected EMBO Member. He served as National Counsellor for Science and Technology to the Portuguese Prime Minister and founded Yscience (www.yscience.pt), a pilot non-profit initiative that brings together scientists to promote science education in young children. His main research interest is the spatiotemporal control of chromosome segregation during cell division.
Principal Investigator/Group Leader, Chromosome Instability and Dynamics Lab, Instituto de Investigacao e Inovacao em Saude da Universidade do Porto Rua Alfredo Allen, Porto, Portugal
Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Gottingen, Germany
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