Mitosis and Meiosis Part A - 1st Edition - ISBN: 9780128141441, 9780128141458

Mitosis and Meiosis Part A, Volume 144

1st Edition

Serial Volume Editors: Helder Maiato Melina Schuh
eBook ISBN: 9780128141458
Hardcover ISBN: 9780128141441
Imprint: Academic Press
Published Date: 26th May 2018
Page Count: 494
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Table of Contents


Helder Maiato and Melina Schuh

    1. Assays for the spindle assembly checkpoint in cell culture
    2. Chiara Marcozzi and Jonathon Pines

    3. Quantitative methods to measure aneuploidy and chromosomal instability
    4. Kristina M. Godek and Duane A. Compton

    5. Dissecting the role of the tubulin code in mitosis
    6. Luísa T. Ferreira, Ana C. Figueiredo, Bernardo Orr, Danilo Lopes and Helder Maiato

    7. Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis
    8. Kara L. McKinley

    9. Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells
    10. Bramwell G. Lambrus, Tyler C. Moyer and Andrew J. Holland

    11. Small molecule tools in mitosis research
    12. Franziska Teusel, Lars Henschke and Thomas U. Mayer

    13. Optogenetic control of mitosis with photocaged chemical dimerizers
    14. Huaiying Zhang, David M. Chenoweth and Michael A. Lampson

    15. Measuring mitotic forces
    16. Anna A. Ye and Thomas J. Maresca

    17. Employing the one-cell C. elegans embryo to study cell division processes
    18. Neil Hattersley, Pablo Lara-Gonzalez, Dhanya Cheerambathur, Sebastian Gomez, Taekyung Kim, Bram Prevo, Renat Khaliullin, Kian-Yong Lee, Midori Ota, Rebecca Green, Karen Oegema and Arshad Desai

    19. A cell-free system of Drosophila egg explants supporting native mitotic cycles
    20. Jorge de-Carvalho, Ojas Deshpande, Catarina Nabais and Ivo A. Telley

    21. Living Xenopus oocytes, eggs, and embryos as models for cell division
    22. Ani Varjabedian, Angela Kita and William Bement

    23. Spreading of chromosomes from mouse oocytes and mammalian cultured cells for light microscopic analysis
    24. Mariana C.C. Silva, Gordana Wutz, Kikuë Tachibana and Jan-Michael Peters

    25. In vitro reconstitution of lateral to end-on conversion of kinetochore–microtubule attachments
    26. Manas Chakraborty, Ekaterina Tarasovetc and Ekaterina L. Grishchuk

    27. Isolation of mitotic chromosomes from vertebrate cells and characterization of their proteome by mass spectrometry
    28. Itaru Samejima and William C. Earnshaw

    29. Purification of kinetochores from the budding yeast Saccharomyces cerevisiae
    30. Amitabha Gupta, Rena K. Evans, Lori B. Koch, Aimee J. Littleton and Sue Biggins

    31. Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae
    32. Rokas Grigaitis, Aitor Susperregui, Philipp Wild and Joao Matos

    33. Single-nucleus Hi-C of mammalian oocytes and zygotes
    34. Johanna Gassler, Ilya M. Flyamer and Kikuë Tachibana

    35. Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells
    36. Hidetaka Miyauchi, Hiroshi Ohta and Mitinori Saitou

    37. Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos
    38. Greg FitzHarris, John Carroll and Karl Swann

    39. Single cell genomics to study DNA and chromosome changes in human gametes and embryos
    40. Robert C. Blanshard, Chongyi Chen, X. Sunney Xie and Eva R. Hoffmann

    41. Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes

Hirohisa Kyogoku, Shuhei Yoshida and Tomoya S. Kitajima


Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Analyzing the Spindle Assembly Checkpoint in human cell culture, an Analysis of CIN, a Functional analysis of the tubulin code in mitosis, Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis, Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells, Small Molecule Tools in Mitosis Research, Optogenetic control of mitosis with photocaged chemical, and more.

Key Features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics


Researchers and professionals in Cell Biology field


No. of pages:
© Academic Press 2018
Academic Press
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Ratings and Reviews

About the Serial Volume Editors

Helder Maiato Serial Volume Editor

Helder Maiato graduated in Biochemistry and holds a PhD in Biomedical Sciences from the University of Porto. He was a visiting PhD student at the University of Edinburgh (UK) and a Post-doctoral Research Affiliate at the New York State Department of Health (USA). At present, he is a Principal Investigator at the Institute for Research and Innovation in Health (i3S) and Invited Professor at the Faculty of Medicine of the University of Porto (Portugal). In 2015 he was honoured with the Young Investigator Award from the Louis-Jeantet Foundation and was recently elected EMBO Member. He served as National Counsellor for Science and Technology to the Portuguese Prime Minister and founded Yscience (, a pilot non-profit initiative that brings together scientists to promote science education in young children. His main research interest is the spatiotemporal control of chromosome segregation during cell division.

Affiliations and Expertise

Principal Investigator/Group Leader, Chromosome Instability and Dynamics Lab, Instituto de Investigacao e Inovacao em Saude da Universidade do Porto Rua Alfredo Allen, Porto, Portugal

Melina Schuh Serial Volume Editor

Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany

Affiliations and Expertise

Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Gottingen, Germany