Mitosis and Meiosis Part A - 1st Edition - ISBN: 9780128141441

Mitosis and Meiosis Part A, Volume 144

1st Edition

Serial Volume Editors: Helder Maiato Melina Schuh
Hardcover ISBN: 9780128141441
Imprint: Academic Press
Published Date: 1st June 2018
Page Count: 362
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Table of Contents

1. Analysing the Spindle Assembly Checkpoint in human cell culture
Jonathon Pines
2. Analysis of CIN
Duane Compton
3. Functional analysis of the tubulin code in mitosis
Helder Maiato
4. Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis
Kara L. McKinley
5. Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells
Andrew Jon Holland
6. Small Molecule Tools in Mitosis Research
Thomas U. Mayer
7. Optogenetic control of mitosis with photocaged chemical dimerizers
Michael Lampson
8. Measuring forces in living cells
Tom Maresca
9. Employing the One-Cell C. elegans Embryo to Study Cell Division Processes
Arshad Desai, Neil Hattersley and Pablo Lara-Gonzalez
10. A cell-free system of Drosophila egg explants supporting native mitotic cycles
Ivo Andreas Telley
11. Living Xenopus oocytes, eggs and embryos as models for cell division
Bill Bement
12. Chromosome spreads in somatic cells and oocytes OR TIRF to study cohesin
Jan-Michael Peters
13. In Vitro Reconstitution of Microtubule Lateral to End-on Conversion Assisted by the Kinetochore Kinesin CENP-E
Ekaterina L. Grishchuk and Manas Chakraborty
14. Isolation of mitotic chromosomes from vertebrate cells and characterisation of their proteome by mass spectrometry
William C. Earnshaw and Itaru Samejima
15. Isolation of yeast kinetochores
Sue Biggins
16. Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae.
Joao Matos
17. Single-nucleus Hi-C in oocytes and zygotes
Kikue Tachibana-Konwalski, Johanna Gassler and Ilya Flyamer
18. Induction of Fetal Primary Oocytes and the Meiotic Prophase from Mouse Pluripotent Stem Cells
Mitinori Saitou
19. Electrical-assisted microinjection for analysis of fertilisation and cell division in mammalian oocytes and early embryos
Greg FitzHarris and Karl Swann
20. Single cell genomics to study DNA and chromosome changes in human gametes and embryos
Eva R. Hoffmann
21. Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes
Tomoya Kitajima


Description

Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Mitotic live cell imaging at different time scales, The characterization of mitotic spindle by multi-mode correlative microscopy, STED microscopy of mitosis, Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture, quantification of three-dimensional spindle architecture, Imaging based assays for mitotic chromosome condensation and dynamics, and more.

Key Features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics

Readership

Researchers and professionals in Cell Biology field


Details

No. of pages:
362
Copyright:
© Academic Press 2018
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128141441

Ratings and Reviews


About the Serial Volume Editors

Helder Maiato Serial Volume Editor

Principal Investigator/Group Leader, Chromosome Instability & Dynamics Lab (room 203-S1) i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto Rua Alfredo Allen, 208 4200–135 Porto, PORTUGAL

Affiliations and Expertise

Principal Investigator/Group Leader, Chromosome Instability and Dynamics Lab, Instituto de Investigacao e Inovacao em Saude da Universidade do Porto Rua Alfredo Allen, Porto, Portugal

Melina Schuh Serial Volume Editor

Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany

Affiliations and Expertise

Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Gottingen, Germany