Mitosis and Meiosis Part A

Mitosis and Meiosis Part A

1st Edition - May 24, 2018

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  • Editors: Helder Maiato, Melina Schuh
  • Hardcover ISBN: 9780128141441
  • eBook ISBN: 9780128141458

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Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Analyzing the Spindle Assembly Checkpoint in human cell culture, an Analysis of CIN, a Functional analysis of the tubulin code in mitosis, Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis, Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells, Small Molecule Tools in Mitosis Research, Optogenetic control of mitosis with photocaged chemical, and more.

Key Features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics


Researchers and professionals in Cell Biology field

Table of Contents

  • Preface
    Helder Maiato and Melina Schuh
    1. Assays for the spindle assembly checkpoint in cell culture
    Chiara Marcozzi and Jonathon Pines
    2. Quantitative methods to measure aneuploidy and chromosomal instability
    Kristina M. Godek and Duane A. Compton
    3. Dissecting the role of the tubulin code in mitosis
    Luísa T. Ferreira, Ana C. Figueiredo, Bernardo Orr, Danilo Lopes and Helder Maiato
    4. Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis
    Kara L. McKinley
    5. Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells
    Bramwell G. Lambrus, Tyler C. Moyer and Andrew J. Holland
    6. Small molecule tools in mitosis research
    Franziska Teusel, Lars Henschke and Thomas U. Mayer
    7. Optogenetic control of mitosis with photocaged chemical dimerizers
    Huaiying Zhang, David M. Chenoweth and Michael A. Lampson
    8. Measuring mitotic forces
    Anna A. Ye and Thomas J. Maresca
    9. Employing the one-cell C. elegans embryo to study cell division processes
    Neil Hattersley, Pablo Lara-Gonzalez, Dhanya Cheerambathur, Sebastian Gomez, Taekyung Kim, Bram Prevo, Renat Khaliullin, Kian-Yong Lee, Midori Ota, Rebecca Green, Karen Oegema and Arshad Desai
    10. A cell-free system of Drosophila egg explants supporting native mitotic cycles
    Jorge de-Carvalho, Ojas Deshpande, Catarina Nabais and Ivo A. Telley
    11. Living Xenopus oocytes, eggs, and embryos as models for cell division
    Ani Varjabedian, Angela Kita and William Bement
    12. Spreading of chromosomes from mouse oocytes and mammalian cultured cells for light microscopic analysis
    Mariana C.C. Silva, Gordana Wutz, Kikuë Tachibana and Jan-Michael Peters
    13. In vitro reconstitution of lateral to end-on conversion of kinetochore–microtubule attachments
    Manas Chakraborty, Ekaterina Tarasovetc and Ekaterina L. Grishchuk
    14. Isolation of mitotic chromosomes from vertebrate cells and characterization of their proteome by mass spectrometry
    Itaru Samejima and William C. Earnshaw
    15. Purification of kinetochores from the budding yeast Saccharomyces cerevisiae
    Amitabha Gupta, Rena K. Evans, Lori B. Koch, Aimee J. Littleton and Sue Biggins
    16. Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae
    Rokas Grigaitis, Aitor Susperregui, Philipp Wild and Joao Matos
    17. Single-nucleus Hi-C of mammalian oocytes and zygotes
    Johanna Gassler, Ilya M. Flyamer and Kikuë Tachibana
    18. Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells
    Hidetaka Miyauchi, Hiroshi Ohta and Mitinori Saitou
    19. Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos
    Greg FitzHarris, John Carroll and Karl Swann
    20. Single cell genomics to study DNA and chromosome changes in human gametes and embryos
    Robert C. Blanshard, Chongyi Chen, X. Sunney Xie and Eva R. Hoffmann
    21. Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes
    Hirohisa Kyogoku, Shuhei Yoshida and Tomoya S. Kitajima

Product details

  • No. of pages: 494
  • Language: English
  • Copyright: © Academic Press 2018
  • Published: May 24, 2018
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128141441
  • eBook ISBN: 9780128141458

About the Serial Volume Editors

Helder Maiato

Helder Maiato graduated in Biochemistry and holds a PhD in Biomedical Sciences from the University of Porto. He was a visiting PhD student at the University of Edinburgh (UK) and a Post-doctoral Research Affiliate at the New York State Department of Health (USA). At present, he is a Principal Investigator at the Institute for Research and Innovation in Health (i3S) and Invited Professor at the Faculty of Medicine of the University of Porto (Portugal). In 2015 he was honoured with the Young Investigator Award from the Louis-Jeantet Foundation and was recently elected EMBO Member. He served as National Counsellor for Science and Technology to the Portuguese Prime Minister and founded Yscience (, a pilot non-profit initiative that brings together scientists to promote science education in young children. His main research interest is the spatiotemporal control of chromosome segregation during cell division.

Affiliations and Expertise

Principal Investigator/Group Leader, Chromosome Instability and Dynamics Lab, Instituto de Investigacao e Inovacao em Saude da Universidade do Porto Rua Alfredo Allen, Porto, Portugal

Melina Schuh

Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany

Affiliations and Expertise

Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Gottingen, Germany

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