Mitochondrial Function, Part B

Mitochondrial Function, Part B

Mitochondrial Protein Kinases, Protein Phosphatases and Mitochondrial Diseases

1st Edition - May 5, 2009

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  • Editors: William Allison, Anne Murphy
  • eBook ISBN: 9780080923505
  • Hardcover ISBN: 9780123746221

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In this second of two new volumes covering mitochondria, methods developed to assess the number and function of nuclear-encoded proteins in the mitochondrion are presented. Chapters focus on the regulation of mitochondrial function and mitochondrial diseases, with a section emphasizing the mitochondrial defects associated with type 2 diabetes. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. With more than 450 volumes published, each volume presents material that is relevant in today's labs, truly an essential publication for researchers in all fields of life sciences.

Key Features

  • New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them
  • Along with a companion volume covering topics including mitochondrial electron transport chain complexes and reactive oxygen species, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction
  • Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods


Researchers, professors, and students of biochemistry, molecular biology and cell biology

Table of Contents

  • Defining the Mitochondrial Proteome
    1. Method to define the mammalian mitochondrial proteome; V. Mootha
    2. Proteome Characterization of Mouse Brain Mitochondria Using Electrospray Ionization Tandem Mass Spectrometry; X.Fang and C. S. Lee
    3. Method to analyze tissue heterogeneity of the mammalian mitochondrial proteome; R. S. Balaban
    4. Predicting proteomes of mitochondria and related organelles from genomic and expressed sequence tag data; D. Gaston, A. Tsausis and A. Roger
    5. MitoP2: the mitochondrial proteome database; H. Prokisch

    Post-Translational Modifications of Mitochondrial Proteins
    6. Reversible protein acetylation in mitochondria; E. Verdin
    7.Post-translational modifications of rat liver mitochondria outer membrane proteins' C. L. Hoppel
    8.Characterization of a novel ser/thr protein phosphatase in mitochondria; Y. Wang
    9. R. Rizzuto
    10.Selective enrichment in phosphopeptides for the identification of phosphorylated mitochondrial proteins; G. Pocsfalvi
    11. Isolation of Regulatory-competent, Phosphorylated Cytochrome c and Cytochrome c Oxidase; M. Hüttemann
    12. Identification of tyrosine phosphorylated proteins by Src kinases in rat brain; M. Salvi
    13. Detection of a mitochondrial kinase complex that mediates an ERK-dependent phosphorylation of mitochondrial protein in steroid biosynthesis; E. J. Podestá
    14. Dual specific phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane; J. Rardin & J. E. Dixon
    15.M. A. Kostiuk and B. O. Keller: “Isolation and identification of palmitoylated proteins in mitochondria using a chemical biology approach; L. G. Berthiaume

    Mitochondrial Dynamics
    16.Mitochondrial fusion and movement using photoactivatable GFP; O. Shirihai
    17. Regulation of mitochondrial fission and fusion by kinase and phosphatase activities. no title yet; S. Strack
    18. Mitochondrial movement and synaptic function in Drosophila; T. L. Schwarz
    19. The use of yeast and a novel biosensor to study mitochondrial ion transport, swelling, and mitophagy; R. J. Scheweyen
    20.Complete Exchange of mtDNA’s between Mouse Cells for Testing the Effects of mtDNA on Tumor Phenotypes; J. Hayashi

    Mitochondrial Function in Pancreatic Beta Cells
    21. Coupling efficiency INS-1E cells; M. D. Brand and C. Affourtit
    22. Methods in the study of substrate-specific differences on mitochondrial function mediating insulin secretion from pancreatic beta cells; B. E. Corkey
    23. Knocking down of UCP 2 in animal models of obesity; L. A.Velloso
    24.Roles of the cytosolic and mitochondrial forms of the malic enzyme on insulin secretion; G. W. Cline
    25. Altered regulation of the PINK1 locus as a link between type 2 diabetes and neurodegeneration; J. A. Timmons
    26. Insulin secretion from pancreatic beta cells is affected deletion of the nicotinamide nucleotide transhydrogenase; R. D. Cox
    27. Determination of oxidative capacity and mitochondrial function in insulin resistance and type 2 diabetes; G. Shulman et al
    28.Mitochondrial ATP production rate; K. S. Nair

Product details

  • No. of pages: 450
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: May 5, 2009
  • Imprint: Academic Press
  • eBook ISBN: 9780080923505
  • Hardcover ISBN: 9780123746221

About the Serial Volume Editors

William Allison

Anne Murphy

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