Description

The first of two new volumes covering mitochondria, this volume presents modern methods that have been developed to examine mitochondrial electron transport chain complexes, iron-sulfur proteins and reactive oxygen species. These new techniques provide investigators with sensitive, original approaches to the study of disease states associated with mitochondrial malfunction. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences.

Key Features

New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them Along with companion volume covering mitochondrial diseases and defects, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Readership

Researchers and students of biochemistry and molecular and cell biology

Table of Contents

Part A: Electron Transport Complexes 1. Visualizing functional flexibility by three dimensional electron microscopy: reconstructing Complex I of the mitochondrial respiratory chain; M. Radermacher 2. Electron tomography of the mitochondrion; T. Frey, G. Perkins, M. Sun 3. Mid-IR vibrational studies of complex I; D. Marshall, P. Rich 4. Electron transfer in respiratory complexes resolved by an ultra-fast freeze-quench approach; N. Belevich, M. Verkhovskya, M. Verkhovsky 5. Use of ruthenium photooxidation techniques to study electron transfer in the cytochrome bc1 complex; F. Millett, B. Durham, C. Yu, L. Yu 6. Use of ruthenium photoreduction techniques to study electron transfer in cytochrome oxidase; F. Millett, B. Durham, R. Gennis 7. Mass spectrometric characterization of subunits of electron transfer complexes that are encoded in the mitochondrial genome; J. Carroll, J. Walker 8. The assembly of respiratory chain complexes studied by GFP-tagged subunits, L. Nijtmans 9. Two-dimensional native electrophoresis for functional assays of mitochondrial complexes; W. Wittig, H. Schägger 10. Reliable assay for complex I in human blood lymphocytes and skin fibroblasts; L. Elly, A. de Wit, W. Sluiter 11. Purification of the cytochrome c reductase/cytochrome c oxidase supercomplex of yeast mitochondria; E. Boekema and H. Braun 12. Supercomplex organization of the yeast mitochondrial respiratory chain complexes and the ADP/ATP carrier proteins; R. Stuart 13. Role of cysteine desulfurase Nfs1 in human mitochondrial Fe-S protein biogenesis; O. Stehling, R. Lill 14. Identification and properties of the novel 2Fe-2S center in the outer membrane protein, mitoNEET; S. Wiley, M. Rardin, J. Dixon 15. Nucleotide-dependent iron-sulfur cluster biogenesis in mitochondria; D. Pa

Details

No. of pages:
502
Language:
English
Copyright:
© 2009
Published:
Imprint:
Academic Press
Print ISBN:
9780080877761
Electronic ISBN:
9780080915333