Mitochondrial Function, Part A

Mitochondrial Function, Part A

Mitochondrial Electron Transport Complexes and Reactive Oxygen Species

1st Edition - April 3, 2009

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  • Editors: William Allison, Immo Scheffler
  • eBook ISBN: 9780080915333
  • Hardcover ISBN: 9780080877761

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Description

The first of two new volumes covering mitochondria, Mitochondrial Function, Part A presents modern methods that have been developed to examine mitochondrial electron transport chain complexes, iron-sulfur proteins and reactive oxygen species. These new techniques provide investigators with sensitive, original approaches to the study of disease states associated with mitochondrial malfunction. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences.

Key Features

  • New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them
  • Along with companion volume covering mitochondrial diseases and defects, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction
  • Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Readership

Researchers and students of biochemistry and molecular and cell biology

Table of Contents

  • Part A: Electron Transport Complexes
    1. Visualizing functional flexibility by three dimensional electron microscopy: reconstructing Complex I of the mitochondrial respiratory chain; M. Radermacher
    2. Electron tomography of the mitochondrion; T. Frey, G. Perkins, M. Sun
    3. Mid-IR vibrational studies of complex I; D. Marshall, P. Rich
    4. Electron transfer in respiratory complexes resolved by an ultra-fast freeze-quench approach; N. Belevich, M. Verkhovskya, M. Verkhovsky
    5. Use of ruthenium photooxidation techniques to study electron transfer in the cytochrome bc1 complex; F. Millett, B. Durham, C. Yu, L. Yu
    6. Use of ruthenium photoreduction techniques to study electron transfer in cytochrome oxidase; F. Millett, B. Durham, R. Gennis
    7. Mass spectrometric characterization of subunits of electron transfer complexes that are encoded in the mitochondrial genome; J. Carroll, J. Walker
    8. The assembly of respiratory chain complexes studied by GFP-tagged subunits, L. Nijtmans
    9. Two-dimensional native electrophoresis for functional assays of mitochondrial complexes; W. Wittig, H. Schägger
    10. Reliable assay for complex I in human blood lymphocytes and skin fibroblasts; L. Elly, A. de Wit, W. Sluiter
    11. Purification of the cytochrome c reductase/cytochrome c oxidase supercomplex of yeast mitochondria; E. Boekema and H. Braun
    12. Supercomplex organization of the yeast mitochondrial respiratory chain complexes and the ADP/ATP carrier proteins; R. Stuart
    13. Role of cysteine desulfurase Nfs1 in human mitochondrial Fe-S protein biogenesis; O. Stehling, R. Lill
    14. Identification and properties of the novel 2Fe-2S center in the outer membrane protein, mitoNEET; S. Wiley, M. Rardin, J. Dixon
    15. Nucleotide-dependent iron-sulfur cluster biogenesis in mitochondria; D. Pain
    16. Preparation of samples for EPR and Mössbauer spectroscopy of intact Saccharomyces cerrevisiae mitochondria; J. Garber-Morales, R. Miao, G. Holmes-Hampton, P. Lindahl
    17. Fluorescence correlation spectroscopy to probe mitochondrial mobility and intramatrix protein diffusion; P. Willems, W. Koopman
    18. Characterization of alternative complex I of Plasmodium falciparum mitochondria; N. Fisher, A. J. Warman, S. Ward, G. Biagini
    19. Analysis of respiratory chain complex assembly using radiolabeled nuclear- and mitochondrial-encoded subunits; M. McKenzie, M. Lazarou, M. Ryan

    Part B: Reactive Oxygen Species
    20. Measuring redox changes to mitochondrial protein thiols using Redox DIGE; T. Hurd, K. Lilley, M. Murphy
    21.Measuring the effects of Hepatitis C proteins on mitochondrial electron transport and production of reactive oxygen species; S. Weinman
    22. Paraquat induced production of reactive oxygen species in brain mitochondria; D. Drechsel, M. Patel
    23. The uptake and interactions of the redox cycler paraquat with mitochondria; H. Cocheme; M. Murphy.
    24. Superoxide production by rat brain and skeletal muscle mitochondria; W. Kunz
    25. Quantitation, localization, and tissue specificities of mitochondrial reactive oxygen species production; C. Mathews
    26. The reaction mechanisms of electron transfer and superoxide generation in the cytochrome nc1 complex; D. Xia, S. Yang, Y. Yin, L. Yu; C. Yu
    27. Measurement of superoxide formation by mitochondrial complex I of Yarrowia lipolytica; S. Dröse, A. Galkin, U. Brandt
    28. An improved method for introducing point mutations into the mitochondrial cytochrme b gene to facilitate studying the role of cytochrome b in the formation of reactive oxygen species; B. Trumpower

Product details

  • No. of pages: 502
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: April 3, 2009
  • Imprint: Academic Press
  • eBook ISBN: 9780080915333
  • Hardcover ISBN: 9780080877761

About the Serial Volume Editors

William Allison

Immo Scheffler

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