Description

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* This volume covers methodology used in * Mitochondrial gene expression * Mitochondrial genetics and gene manipulation * Mitochondrial diseases and aging

Readership

Biochemists, geneticists, microbiologists, biotechnologists, genetic engineers, analytical chemists, clinical chemists, and industrial researchers working on protein products; plant scientists involved in plant breeding and pathology.

Table of Contents

In Vivo and in Vitro Approaches to Study of Mitochondrial Gene Expression: V. Micol, P. Fernandez-Silva, and G. Attardi, In Vivo Footprinting of Human Mitochondrial DNA in Cultured Cell Systems. C.S. Madsen, S.C. Ghivizzani, C.V. Ammini, M.R. Nelen, and W.W. Hauswirth, Genomic Footprinting of Mitochondrial DNA: I. In Organello Analysis of Protein–Mitochondrial DNA Interactions in Bovine Mitochondria. C.V. Ammini, S.C. Ghivizzani, C.S. Madsen, and W.W. Hauswirth, Genomic Footprinting of Mitochondrial DNA: II. In Vivo Analysis of Protein–Mitochondrial DNA Interactions in Xenopus laevis Eggs and Embryos. R.O. Poyton, G. Bellus, E.E. McKee, K.A. Sevarino, and B. Goehring, In Organello Mitochondrial Protein and RNA Synthesis Systems from Saccharomyces cerevisiae. G.L.Gaines, III, In Organello RNA Synthesis System from HeLa Cells. J.A. Enriquez, A. Perez-Martos, M.J. Lopez-Perez, and J. Montoya, In Organello RNA Synthesis System from Mammalian Liver and Brain. D.A. Mangus and J.A. Jaehning, Transcription in Vitro with Saccharomyces cerevisiae Mitochondrial RNA Polymerase. P.S. Perlman and M. Podar, Reactions Catalyzed byGroup II Introns in Vitro. K.R. Groom, Y.L. Dang, G.-J. Gao, Y.C. Lou, N.C. Martin, C.A. Wise, and M.J. Morales, Genetic and Biochemical Approaches for Analysis of Mitochondrial RNase P from Saccharomyces cerevisiae. L. Simpson, G.C. Frech, andD.A. Maslov, RNA Editing in Trypanosomatid Mitochondria. C. Ausenda and A. Chomyn, Purification of Mitochondrial DNA from Human Cell Cultures and Placenta. P. Fernandez-Silva, V. Micol, and G. Attardi, Mitochondrial DNA Transcription Initiation and Termination Using Mitochondrial Lysates from Cultured Human Cells. G.S. Shadel and D.A. Clayton, Mapping Promoters in Displacement-Loop Region of Vertebrate Mitochondrial DNA.

Details

No. of pages:
621
Language:
English
Copyright:
© 1996
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883779
Print ISBN:
9780121821654

About the serial-volume-editors

Giuseppe Attardi

Affiliations and Expertise

Division of Biology, California Institute of Technology, Pasadena, California, U.S.A.

Anne Chomyn

Affiliations and Expertise

Division of Biology, California Institute of Technology, Pasadena, California, U.S.A.

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY