Mitochondria, Volume 80

2nd Edition

Editors: Liza Pon Eric Schon
Hardcover ISBN: 9780125441735
eBook ISBN: 9780080489377
Imprint: Academic Press
Published Date: 10th April 2007
Page Count: 944
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Table of Contents

Isolation and Subfractionation of Mitochondria

  1. Isolation and Subfractionation of Mitochondria in Animal Cells
  2. Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae
  3. Isolation and Subfractionation of Mitochondria from Plants

Biochemical Assays of Mitochondrial Activity

  1. Biochemical Assays of Respiratory Chain Complex Activity
  2. Polarographic Assays of Respiratory Chain Complex Activity
  3. Optical Imaging Techniques (Histochemical, Immunohistochemical and In Situ Hybridization Staining Methods) to Visualize Mitochondria
  4. Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues
  5. Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures
  6. Assays of Fatty Acid ƒÒ-Oxidation Activity
  7. Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc
  8. Assays of Cardiolipin Levels
  9. Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems
  10. Methods for Studying Fe/S Cluster Protein Maturation in Yeast Mitochondria

Assays for Mitochondrial Respiratory Activity and Permeability in Living Cells

  1. Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores
  2. Biosensors for the Detection of Calcium and pH
  3. Measurement of Membrane Permeability and the Permeability Transition of Mitochondria
  4. Luciferase Expression for ATP Imaging: Application to Cardiac Myocytes

Oxidative Stress Measurements

  1. Measurement of Reactive Oxygen Species in Cells and Mitochondria
  2. Measurements of the Antioxidant Enzyme Activities of Superoxide D


This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.


Researchers and graduate students in cell biology, developmental biology, and genetics. Academic and research libraries, pharmaceutical and biotechnology companies, and private research facilities.


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© Academic Press 2007
Academic Press
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About the Editors

Liza Pon Editor

Eric Schon Editor

Affiliations and Expertise

Columbia University, Neurology Department, New York, U.S.A.