Mitochondria - 2nd Edition - ISBN: 9780125441735, 9780080489377

Mitochondria, Volume 80

2nd Edition

Editors: Liza Pon Eric Schon
eBook ISBN: 9780080489377
Hardcover ISBN: 9780125441735
Imprint: Academic Press
Published Date: 10th April 2007
Page Count: 944
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Table of Contents

Isolation and Subfractionation of Mitochondria

  1. Isolation and Subfractionation of Mitochondria in Animal Cells
  2. Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae
  3. Isolation and Subfractionation of Mitochondria from Plants

Biochemical Assays of Mitochondrial Activity

  1. Biochemical Assays of Respiratory Chain Complex Activity
  2. Polarographic Assays of Respiratory Chain Complex Activity
  3. Optical Imaging Techniques (Histochemical, Immunohistochemical and In Situ Hybridization Staining Methods) to Visualize Mitochondria
  4. Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues
  5. Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures
  6. Assays of Fatty Acid ƒÒ-Oxidation Activity
  7. Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc
  8. Assays of Cardiolipin Levels
  9. Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems
  10. Methods for Studying Fe/S Cluster Protein Maturation in Yeast Mitochondria

Assays for Mitochondrial Respiratory Activity and Permeability in Living Cells

  1. Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores
  2. Biosensors for the Detection of Calcium and pH
  3. Measurement of Membrane Permeability and the Permeability Transition of Mitochondria
  4. Luciferase Expression for ATP Imaging: Application to Cardiac Myocytes

Oxidative Stress Measurements

  1. Measurement of Reactive Oxygen Species in Cells and Mitochondria
  2. Measurements of the Antioxidant Enzyme Activities of Superoxide Dismutase, Catalase, and Glutathione Peroxidase
  3. Methods for Measuring the Regulation of Respiration by Nitric Oxide
  4. Methods for Determining the Modification of Protein Thiols by Reactive Lipids

Mitochondrial Genes and Gene Expression

  1. Detection of Mutations in mtDNA
  2. Diagnostic Assays for Defects in mtDNA Replication and Transcription in Yeast and Humans
  3. Microdissection and Analytical PCR for the Investigation of mtDNA Lesions
  4. Transmitochondrial Technology in Animal Cells
  5. Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas Reinhardtii Mitochondria
  6. Generation of Transmitochondrial Mice: Development of Xenomitochondrial Mice to Model Neurodegenerative Diseases
  7. In vivo and In organello Analysis of Mitochondrial Translation Assays for Mitochondrial Morphology and Motility
  8. Visualization of Mitochondria in Budding Yeast
  9. Visualization and Quantification of Mitochondrial Dynamics in Living Animal Cells
  10. Cell-Free Assays for Mitochodnrial-Cytoskeletal Interactions
  11. Assays to Measure Mitochondrial Fusion In Vitro and the GTPase Activity of Mitochondrial Fission Proteins

Methods to Determine Protein Localization to Mitochondria

  1. Electrophoretic Methods to Isolate Protein Complexes from Mitochondria
  2. Analysis of Protein-Protein Interactions in Mitochondria
  3. Analysis and Prediction of Mitochondrial Targeting Signals
  4. Import of Proteins into Mitochondria

Appendices Appendix 1: Basic Properties of Mitochondria Appendix 2: Direct and Indirect Inhibitors of Mitochondrial ATP Synthesis Appendix 3: Linearized Maps of Mitochondrial Genomes from Representative Organisms Appendix 4: Mitochondrial Genetic Codes in Various Organisms Appendix 5: Gene Products Present in Mitochondria of Yeast and Animal Cells Appendix 6: Changes in the Mitochondrial Transcriptome and Proteome Under Various Stresses and Growth Conditions


Description

This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.

Readership

Researchers and graduate students in cell biology, developmental biology, and genetics. Academic and research libraries, pharmaceutical and biotechnology companies, and private research facilities.


Details

No. of pages:
944
Language:
English
Copyright:
© Academic Press 2007
Published:
Imprint:
Academic Press
eBook ISBN:
9780080489377
Hardcover ISBN:
9780125441735

About the Editors

Liza Pon Editor

Eric Schon Editor

Affiliations and Expertise

Columbia University, Neurology Department, New York, U.S.A.