This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.


Researchers and graduate students in cell biology, developmental biology, and genetics. Academic and research libraries, pharmaceutical and biotechnology companies, and private research facilities.

Table of Contents

Isolation and Subfractionation of Mitochondria 1. Isolation and Subfractionation of Mitochondria in Animal Cells 2. Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae 3. Isolation and Subfractionation of Mitochondria from Plants Biochemical Assays of Mitochondrial Activity 4. Biochemical Assays of Respiratory Chain Complex Activity 5. Polarographic Assays of Respiratory Chain Complex Activity 6. Optical Imaging Techniques (Histochemical, Immunohistochemical and In Situ Hybridization Staining Methods) to Visualize Mitochondria 7. Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues 8. Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures 9. Assays of Fatty Acid ƒÒ-Oxidation Activity 10. Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc 11. Assays of Cardiolipin Levels 12. Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems 13. Methods for Studying Fe/S Cluster Protein Maturation in Yeast Mitochondria Assays for Mitochondrial Respiratory Activity and Permeability in Living Cells 14. Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores 15. Biosensors for the Detection of Calcium and pH 16. Measurement of Membrane Permeability and the Permeability Transition of Mitochondria 17. Luciferase Expression for ATP Imaging: Application to Cardiac Myocytes Oxidative Stress Measurements 18. Measurement of Reactive Oxygen Species in Cells and Mitochondria 19. Measurements of the Antioxidant Enzyme Activities of Superoxide D


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© 2007
Academic Press
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About the editors

Eric Schon

Affiliations and Expertise

Columbia University, Neurology Department, New York, U.S.A.