Description

This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies.

Key Features

Key Features * Isolation of mitochondria from commonly used cells and tissues * Assays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeleton * Biochemical and optical methods for studying protein-protein interactions in mitochondria * Approaches to studying mitochondrial replication, transcription, and translation * Transmitochondrial technologies * Methods in microassay data analysis

Readership

Researchers and graduate students in cell biology, developmental biology, and genetics. Academic and research libraries, pharmaceutical and biotechnology companies, and private research facilities.

Table of Contents

Contributors. Preface. Isolation and Subfractionation of Mitocondria from Animal Cells and Tissue Culture Lines, F. Pallotti and G. Lenaz. Isolation and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae, K. Dierkert, A.I.P.M. de Kroon, G. Kispal, and R. Lill. Isolation and Subfractionation of Mitochondria from Plants, A.H. Miller, A. Liddell, and C.J. Leaver. Assessing Functional Integrity of Mitochondria in Vitro and in Vivo, M.D. Esposti. Assaying Mitochondrial Respiratory Complex Activity in Mitochondria Isolated from Human Cells and Tissues, M.A. Birch-Machin and D.M. Turnbull. In Vivo Measurements of Respiration Control by Cytochrome c Oxidase and in Situ Analysis of Oxidative Phosphorylation, G. Villani and G. Attardi. Assay of Mitochondrial ATP Synthesis in Animal Cells, G. Manfredi, A. Spinazzola, N. Checcarelli, and A. Naini. Measurement of Membrane Permeability and Permeability Transition of Mitochondria, N. Zamzami, C. Maisse, D. Métivier, and G. Kroemer. Assaying Actin-Binding Activity of Mitochondria in Yeast, I.R. Boldogh and L.A. Pon. Analysis and Prediction of Mitochondrial Targeting Peptides, O. Emanuelsson, G. von Heijne, and G. Schneider. Assaying Protein Import into Mitochondria, M.T. Ryan, W. Voos, and N. Pfanner. Analysis of Protein-Protein Interactions in Mitochondria by Coimmunoprecipitation and Chemical Cross-Linking, J.M. Herrmann, B. Westermann, and W. Neupert. Blue-Native Gels to Isolate Protein Complexes from Mitochondria, H. Schägger. Application of Electron Tomography to Mitochondrial Research, C.A. Mannella. Epitope Taggi

Details

No. of pages:
519
Language:
English
Copyright:
© 2001
Published:
Imprint:
Academic Press
Print ISBN:
9780125441698
Electronic ISBN:
9780080574455

Reviews

@qu:"This is an extremely high quality offering that is really unique...It should serve as an invaluable reference for a wide audience of biomedical researchers." @source:--DOODY'S PUBLISHING REVIEWS