Microtubules, in vitro

Microtubules, in vitro

2nd Edition - August 20, 2013

Write a review

  • Editors: John Correia, Leslie Wilson
  • Hardcover ISBN: 9780124077577
  • eBook ISBN: 9780124078888

Purchase options

Purchase options
Available
DRM-free (EPub, PDF, Mobi)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order

Description

There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This Second Edition brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.

Key Features

  • Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research
  • Combines classical approaches and modern technologies
  • Presents easy-to-use protocols and thorough background information, compiled by leaders in the field

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

  • Series Page
    Contributors
    Preface

    1. Site-Specific Fluorescent Labeling of Tubulin
    Abstract
    Introduction
    Acknowledgments
    References

    2. Measuring Microtubule Persistence Length Using a Microtubule Gliding Assay
    Abstract
    Introduction
    Summary
    Acknowledgments
    References

    3. Structural Studies of the Doublecortin Family of MAPs
    Abstract
    Introduction
    Acknowledgments
    References

    4. Detection and Quantification of Microtubule Detachment from Centrosomes and Spindle Poles
    Abstract
    Introduction
    Summary
    Acknowledgments
    References

    5. Regulation of Tubulin Expression by Micro-RNAs: Implications for Drug Resistance
    Abstract
    Introduction and Rationale
    Summary
    References

    6. Determining the Structure–Mechanics Relationships of Dense Microtubule Networks with Confocal Microscopy and Magnetic Tweezers-Based Microrheology
    Abstract
    Introduction
    Summary
    Acknowledgments
    References

    7. Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes
    Abstract
    Introduction
    Conclusion
    Acknowledgments
    References

    8. Going Solo: Measuring the Motions of Microtubules with an In Vitro Assay for TIRF Microscopy
    Abstract
    Introduction
    Concluding Remarks
    Acknowledgments
    References

    9. Analysis of Microtubules in Isolated Axoplasm from the Squid Giant Axon
    Abstract
    Preparation of Axoplasm
    Analysis of Axoplasmic Microtubule Dynamics
    Biochemistry of Axoplasmic Microtubules
    Immunohistochemistry of Axoplasmic Microtubules
    References

    10. Imaging GTP-Bound Tubulin: From Cellular to In Vitro Assembled Microtubules
    Abstract
    Introduction
    Acknowledgments
    References

    11. Tubulin-Specific Chaperones: Components of a Molecular Machine That Assembles the α/β Heterodimer
    Abstract
    Introduction
    References

    12. Heterotrimeric G Proteins and Microtubules
    Abstract
    Introduction
    Concluding Comments
    Acknowledgments
    References

    13. Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro
    Abstract
    Introduction
    Discussion and Summary
    Acknowledgments
    References

    14. Measurement of In Vitro Microtubule Polymerization by Turbidity and Fluorescence
    Abstract
    Background and Theory
    Materials and Equipment
    Methods
    References

    15. Live-Cell Imaging of Microtubules and Microtubule-Associated Proteins in Arabidopsis thaliana
    Abstract
    Introduction
    Concluding Comments
    Acknowledgment
    References

    16. Investigating Tubulin Posttranslational Modifications with Specific Antibodies
    Abstract
    Introduction
    Concluding Remarks
    Acknowledgments
    References

    17. Purification and Assembly of Bacterial Tubulin BtubA/B and Constructs Bearing Eukaryotic Tubulin Sequences
    Abstract
    Introduction and Rationale
    Conclusions
    Acknowledgments
    References

    18. Microtubule-Associated Proteins and Tubulin Interaction by Isothermal Titration Calorimetry
    Abstract
    Introduction
    Conclusion
    Acknowledgment
    References

    19. Methods for Studying Microtubule Binding Site Interactions: Zampanolide as a Covalent Binding Agent
    Abstract
    Introduction
    Summary
    Acknowledgments
    References

    20. Studying Kinetochore-Fiber Ultrastructure Using Correlative Light-Electron Microscopy
    Abstract
    Introduction
    Acknowledgments
    References

    21. Fluorescence-Based Assays for Microtubule Architecture
    Abstract
    Introduction
    Acknowledgments
    References

    22. Structure–Function Analysis of Yeast Tubulin
    Abstract
    Introduction
    Acknowledgments
    References

    23. Using MTBindingSim as a Tool for Experimental Planning and Interpretation
    Abstract
    Introduction
    Conclusion
    References

    24. Imaging Individual Spindle Microtubule Dynamics in Fission Yeast
    Abstract
    Introduction
    Conclusion
    Acknowledgments
    References

    Index
    Volumes in Series

Product details

  • No. of pages: 440
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: August 20, 2013
  • Imprint: Academic Press
  • Hardcover ISBN: 9780124077577
  • eBook ISBN: 9780124078888

About the Editors

John Correia

Affiliations and Expertise

University of Mississippi Medical Center, Jackson, USA

Leslie Wilson

University of California, Santa Barbara, USA

Affiliations and Expertise

University of California, Santa Barbara, USA

Ratings and Reviews

Write a review

There are currently no reviews for "Microtubules, in vitro"