
Microtubules, in vitro
Description
Key Features
- Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research
- Combines classical approaches and modern technologies
- Presents easy-to-use protocols and thorough background information, compiled by leaders in the field
Readership
Table of Contents
Series Page
Contributors
Preface1. Site-Specific Fluorescent Labeling of Tubulin
Abstract
Introduction
Acknowledgments
References2. Measuring Microtubule Persistence Length Using a Microtubule Gliding Assay
Abstract
Introduction
Summary
Acknowledgments
References3. Structural Studies of the Doublecortin Family of MAPs
Abstract
Introduction
Acknowledgments
References4. Detection and Quantification of Microtubule Detachment from Centrosomes and Spindle Poles
Abstract
Introduction
Summary
Acknowledgments
References5. Regulation of Tubulin Expression by Micro-RNAs: Implications for Drug Resistance
Abstract
Introduction and Rationale
Summary
References6. Determining the Structure–Mechanics Relationships of Dense Microtubule Networks with Confocal Microscopy and Magnetic Tweezers-Based Microrheology
Abstract
Introduction
Summary
Acknowledgments
References7. Studying Mitochondria and Microtubule Localization and Dynamics in Standardized Cell Shapes
Abstract
Introduction
Conclusion
Acknowledgments
References8. Going Solo: Measuring the Motions of Microtubules with an In Vitro Assay for TIRF Microscopy
Abstract
Introduction
Concluding Remarks
Acknowledgments
References9. Analysis of Microtubules in Isolated Axoplasm from the Squid Giant Axon
Abstract
Preparation of Axoplasm
Analysis of Axoplasmic Microtubule Dynamics
Biochemistry of Axoplasmic Microtubules
Immunohistochemistry of Axoplasmic Microtubules
References10. Imaging GTP-Bound Tubulin: From Cellular to In Vitro Assembled Microtubules
Abstract
Introduction
Acknowledgments
References11. Tubulin-Specific Chaperones: Components of a Molecular Machine That Assembles the α/β Heterodimer
Abstract
Introduction
References12. Heterotrimeric G Proteins and Microtubules
Abstract
Introduction
Concluding Comments
Acknowledgments
References13. Purification and Biophysical Analysis of Microtubule-Severing Enzymes In Vitro
Abstract
Introduction
Discussion and Summary
Acknowledgments
References14. Measurement of In Vitro Microtubule Polymerization by Turbidity and Fluorescence
Abstract
Background and Theory
Materials and Equipment
Methods
References15. Live-Cell Imaging of Microtubules and Microtubule-Associated Proteins in Arabidopsis thaliana
Abstract
Introduction
Concluding Comments
Acknowledgment
References16. Investigating Tubulin Posttranslational Modifications with Specific Antibodies
Abstract
Introduction
Concluding Remarks
Acknowledgments
References17. Purification and Assembly of Bacterial Tubulin BtubA/B and Constructs Bearing Eukaryotic Tubulin Sequences
Abstract
Introduction and Rationale
Conclusions
Acknowledgments
References18. Microtubule-Associated Proteins and Tubulin Interaction by Isothermal Titration Calorimetry
Abstract
Introduction
Conclusion
Acknowledgment
References19. Methods for Studying Microtubule Binding Site Interactions: Zampanolide as a Covalent Binding Agent
Abstract
Introduction
Summary
Acknowledgments
References20. Studying Kinetochore-Fiber Ultrastructure Using Correlative Light-Electron Microscopy
Abstract
Introduction
Acknowledgments
References21. Fluorescence-Based Assays for Microtubule Architecture
Abstract
Introduction
Acknowledgments
References22. Structure–Function Analysis of Yeast Tubulin
Abstract
Introduction
Acknowledgments
References23. Using MTBindingSim as a Tool for Experimental Planning and Interpretation
Abstract
Introduction
Conclusion
References24. Imaging Individual Spindle Microtubule Dynamics in Fission Yeast
Abstract
Introduction
Conclusion
Acknowledgments
ReferencesIndex
Volumes in Series
Product details
- No. of pages: 440
- Language: English
- Copyright: © Academic Press 2013
- Published: August 20, 2013
- Imprint: Academic Press
- Hardcover ISBN: 9780124077577
- eBook ISBN: 9780124078888