Microscope Image Processing

Microscope Image Processing

2nd Edition - July 1, 2022

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  • Editors: Fatima Merchant, Kenneth Castleman
  • Paperback ISBN: 9780128210499

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Microscope Image Processing, Second Edition, introduces the basic fundamentals of image formation in microscopy including the importance of image digitization and display, which are key to quality visualization. Image processing and analysis are discussed in detail to provide readers with the tools necessary to improve the visual quality of images, and to extract quantitative information. Basic techniques such as image enhancement, filtering, segmentation, object measurement, and pattern recognition cover concepts integral to image processing. In addition, chapters on specific modern microscopy techniques such as fluorescence imaging, multispectral imaging, three-dimensional imaging and time-lapse imaging, introduce these key areas with emphasis on the differences among the various techniques.The new edition discusses recent developments in microscopy such as light sheet microscopy, digital microscopy, whole slide imaging, and the use of deep learning techniques for image segmentation and analysis with big data image informatics and management.Microscope Image Processing, Second Edition, is suitable for engineers, scientists, clinicians, post-graduate fellows and graduate students working in bioengineering, biomedical engineering, biology, medicine, chemistry, pharmacology and related fields, who use microscopes in their work and would like to understand the methodologies and capabilities of the latest digital image processing techniques or desire to develop their own image processing algorithms and software for specific applications.

Key Features

  • Presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms
  • Each chapter includes in-depth analysis of methods coupled with the results of specific real-world experiments
  • Co-edited by Kenneth R. Castleman, world-renowned pioneer in digital image processing and author of two seminal textbooks on the subject


Engineers, scientists, post-graduates and graduate students working in bioengineering, biomedical engineering, biology, medicine, chemistry, pharmacology and related fields

Table of Contents

  • 1. Introduction
    2. Fundamentals of Microscopy
    3. Image Digitization and Display
    4. Geometric Transformations
    5. Image Enhancement
    6. Morphological Image Processing
    7. Image Segmentation
    8. Object Measurement
    9. Object Classification
    10. Fluorescence Imaging
    11. Multispectral Imaging
    12. Three-Dimensional Imaging
    13. Time-Lapse Imaging
    14. Digital Microscopy
    15. Super Resolution Microscopy
    16. Image Informatics
    17. Deep Learning in Microscope Image Analysis
    18. Open Microscopy Platforms and Tool

Product details

  • No. of pages: 576
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: July 1, 2022
  • Imprint: Academic Press
  • Paperback ISBN: 9780128210499

About the Editors

Fatima Merchant

Associate Professor Fatima Merchant works at Computer Engineering Technology and Computational Health Informatics in Houston, TX, USA.

Affiliations and Expertise

Associate Professor, Computer Engineering Technology and Computational Health Informatics, Houston, TX, USA

Kenneth Castleman

Kenneth R. Castleman is the president of Advanced Digital Imaging Research (ADIR), the author of the canonical textbook Digital Image Processing ISBN 0-13-211467-4 and an authority in the field of image processing and pattern recognition. He holds a B.S, M.S. and Ph.D. all in electrical engineering from The University of Texas at Austin. In 1984, he co-founded Perceptive Systems, Inc (PSI) with Don Winkler in League City, TX.

Affiliations and Expertise

President, Advanced Digital Imaging Research (ADIR), TX, USA

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