This new volume of Methods in Cell Biology looks at micropatterning in cell biology and includes chapters on protein photo-patterning on PEG with benzophenone, laser-directed cell printing and dip pen nanolithography. The cutting-edge material in this comprehensive collection is intended to guide researchers for years to come.

Key Features

  • Includes sections on micropatterning in 2D with photomask, maskless micropatterning and 2D nanopatterning
  • Chapters are written by experts in the field
  • Cutting-edge material


Researchers and students in cell, molecular and developmental biology

Table of Contents

Section 1:  Micro-patterning in 2D
Ch1 Stamp-off” to micropattern sparse, multicomponent features
Ravi A. Desai, Natalia M. Rodriguez and Christopher S. Chen
Ch2 Poly(vinyl alcohol)-Micropatterned Surfaces for Manipulation of Mesenchymal Stem Cell Functions
Guoping Chen
Ch3 Patterning of polymeric cell culture substrates
Alexander Welle, Simone Weigel and Özgül Demir Bulut
Ch4 Photopatterning with a Printed Transparency Mask and a Protein-Friendly Photoresist
Jiwoo Kang, Jong-Cheol Choi, Miju Kim, Hong-Ryul Jung and Junsang Doh
Ch5 Plasma Micro-Contact Patterning (PCP): a technique for the precise control of surface patterning at small-scale
Remigio Picone, Buzz Baum and Rachel McKendry
Ch6 Patterning Gradients
Nagaiyanallur V. Venkataraman, Clément V. M. Cremmel, Christian Zink, Rebecca P. Huber and Nicholas D. Spencer

Section 2: Maskless Micropatterning
Ch7 Laser-assisted absorption by photobleaching
Jonathan M. Bélisle, Javier Mazzaferri and Santiago Costantino
Ch8 Micropatterning with a liquid-crystal display (LCD) projector
Kazuyoshi Itoga, Jun Kobayashi, Masayuki Yamato and Teruo Okano
Ch9 Cell patterning by Laser-assisted bioprinting
Raphaël Devillard, Emeline Pagès, Manuela Medina Correa, Virginie Kériquel, Murielle Rémy, Jérôme Kalisky, Muhammad Ali, Bertrand Guillotin and Fabien Guillemot
Ch10 Stereomask lithography for multi-protein patterning
Siwei Zhao, Arnold Chen, Alexander Revzin and Tingrui Pan
Ch11 Fabrication of Multifaceted, Micropatterned Surfaces and Image-Guided Patterning Using Laser Scanning Lithography
Jennifer L. West and John Hundley Slater
Ch12 Print-to-print: Printer-enabled Out-of-cleanroom Multi-object Microprinting Method
Siyuan Xing, Siwei Zhao and Tingrui Pan

Section 3: 2D Nanopatterning
Ch13 Soft/Elastic Nano-Patterned Biointerfaces in the Service of  Cell Biology
Ilia Platzman, Katarzyna M. Gadomska, Jan-Willi Janiesch, I


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© 2014
Academic Press
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