Microfluidics in Cell Biology Part B: Microfluidics in Single Cells

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells

1st Edition - August 27, 2018

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  • Editors: Matthieu Piel, Junsang Doh, Daniel Fletcher
  • Hardcover ISBN: 9780128142820
  • eBook ISBN: 9780128142837

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Description

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more.

Key Features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics

Readership

Researchers and academics in the cell biology field

Table of Contents

  • Section 1 - Microfluidics for micro-organisms
    1. Temperature control and drug delivery for cell division cycle control in fission yeast
    Damien Coudreuse
    2. H2O2 stress response in budding yeast
    Gilles Charvin
    3. Antibiotic resistance in bacteria
    Bob Austin and Julia Bos
    4. Metabolism in bacteria
    Andrew Griffiths and Philippe Nghe
    5. Fluidized beds for bacterial sorting and amplification
    Stephanie Descroix and Jean-Louis Viovy

    Section 2 - Microfluidics for cell culture and cell sorting of mammalian cells
    6. Hydrogel microwells
    Samy Gobaa
    7. Cell sorting with conical filters
    Yong Chen and Olivier Shi
    8. Directed neuronal cell culture
    Catherine Villard and Jean-Louis Viovy

    Section 3 - Microfluidics for cell migration
    9. Immune cells migration in complexe environments
    Michael Sixt
    10. Neutrophiles migration in health and disease
    Daniel Irimia
    11. Cell guidance by physical cues
    Daniel Riveline
    12. Stable gradients in gels of extracellular matrix for cancer cell migration
    Danijela Vignjevic

    Section 4 - Microfluidics for cell mechanics
    13. Size based cell sorting and single cell mechanobiology
    Chwee Teck Lim
    14. High throughput measure of single cell deformability/stiffness
    Jochen Guck
    15. Host-microbe interactions
    John McKinney
    16. Cell confinement and intracellular crowding
    Liam Holt, Morgan Delarue and Oskar Hallatschek

    Section 5 - Microfluidics for Single cell analytics
    17. Quantification of single cell secretion
    Andrew Griffiths
    18. Mechanical cell disruption
    Wenhui Wang

Product details

  • No. of pages: 247
  • Language: English
  • Copyright: © Academic Press 2018
  • Published: August 27, 2018
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128142820
  • eBook ISBN: 9780128142837

About the Serial Volume Editors

Matthieu Piel

Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell’s environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development.

Affiliations and Expertise

Systems Biology of Cell Division and Cell Polarity, Cell Biology and Cancer Department, Institut Curie, Paris, France Institut Pierre Gilles de Gennes for Microfluidics, Paris, France

Junsang Doh

Junsang Doh is an associate professor of Mechanical Engineering/Interdisciplinary Bioscience and Bioengineering (I-Bio) in POSTECH, South Korea. Prof. Doh’s group develops and utilizes engineering tools such as microfabrication/imaging/mechanics to study fundamental aspects of immune cell behaviors, including synapse-based cell-cell interactions and motility under complex microenvironments, in the context of cancer immunotherapy.

Affiliations and Expertise

Associate Professor, Department of Mechanical Engineering, School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology

Daniel Fletcher

Dr. Fletcher and his team develops diagnostic technologies and studies mechanical regulation of membrane and cytoskeleton organization in the context of cell motility, signaling, and host-pathogen interactions. His lab specialize in development of optical microscopy, force microscopy, and microfluidic technologies to understand fundamental organizational principles through both in vitro reconstitution and live cell experiments. Recent work includes investigating the mechano-biochemistry of branched actin network assembly with force microscopy, studying membrane deformation by protein crowding and oligomerization with model membranes, and reconstituting spindle scaling in encapsulated cytoplasmic extracts. The long-term goal of his work is to understand and harness spatial organization for therapeutic applications in cancer and infectious diseases.

Affiliations and Expertise

Purnendu Chatterjee Chair in Engineering Biological Systems, Department of Bioengineering, University of California Berkeley; Scientist and Deputy Division Director, Physical Biosciences Division, Lawrence Berkeley National Laboratory

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