The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology.

This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications.

The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products.

Key Features

  • Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications
  • Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture
  • Provides balanced coverage of microsystems engineering and bioengineering


Academics, Researchers and Scientists working in a variety of fields, including (but not exclusive to) Biomedical Engineering, Materials Science, Microfabrication, Pharmaceuticals, Stem Cells and Regenerative Medicine technologies.

Table of Contents


List of Contributors

Part 1: Materials and Fabrication Methods

Chapter 1. Microfluidic Cell Culture Platforms with Embedded Nanoscale Features

1.1 Introduction

1.2 Engineering of nanoscale features

1.3 Assembly of PDMS-based microfluidic platforms

1.4 Microfluidic platforms with embedded nanoscale features for cell studies

1.5 Summary



Chapter 2. Microvascular Networks for Tissue Engineering

2.1 Introduction

2.2 Characteristics of branched vascular networks

2.3 Fabrication of 2-D microvascular networks

2.4 Fabrication of 3-D microvascular networks

2.5 Microchannel topologies

2.6 Engineering meets biology: toward tissue engineering applications

2.7 Outlook and future challenges



Chapter 3. Microfluidics for Engineering 3D Tissues and Cellular Microenvironments

3.1 Introduction

3.2 Fabricating 3D tissue scaffolds using microfluidics

3.3 Dynamic 3D cell cultures within PDMS microfluidic devices

3.4 Hydrogel-based microfluidic culture devices and tissue scaffolds

3.5 Conclusion and future directions


Chapter 4. Fabrication of Advanced Microcontainer Arrays for Perfused 3D Cell Culture in Microfluidic Bioreactors

4.1 Introduction

4.2 Micromolding of cell container arrays

4.3 Introducing porosity

4.4 Functionalization of cell container arrays

4.5 Integration into microfluidic bioreactors

4.6 Conclusion


Chapter 5. Mechanobiological Approaches for the Control of Cell Motility

5.1 Introduction

5.2 Passive control of cell motility

5.3 Active control of cell motility

5.4 Summary


Chapter 6. Transport Models for Three-Dimensional Cell Culture Systems



No. of pages:
© 2013
William Andrew
eBook ISBN:
Print ISBN:

About the editors

Christopher Bettinger

Department of Materials Science and Engineering, Carnegie Mellon University