Part 1: Introduction
1. Microfluidic Cell Culture Technologies
2. Inertial microfluidics for stem cell isolation
3. Microfluidic Structures for Controlling Stem Cell Microenvironments
4. Microfluidic cell culture platforms with embedded nanoscale features
5. Microfluidic Vascular Networks for Engineered Tissues
6. Direct Write Assembly of Organ Models
7. 3D Bioprinting for Organ Models
Part II: Organ-Specific Disease and Drug Safety Models
8. Microfabricated Kidney Tissue Models
9. Microfluidic Hepatotoxicity Platform
10. Dynamic Gastrointestinal and Pulmonary Organ Models
11. Cardiac Models
12. Neural Tissue Microphysiological Systems
13. Models of the Female Reproductive Tract
14. Microscale Models of Inflammatory Diseases
15. Blood-Retinal Barrier-on-a –chip
16. Human blinking eye-on-a chip
17. Circulating tumor cell cultures for evaluation of anticancer treatment
Part III: Technologies for Sensing, Multiplexed and Interconnected Organ Models
18. Live Cell Analysis Under Shear Flow
19. High Throughput Flow Systems for Cardiovascular Research
20. Phaseguide technology for connected organ models
21. Electromagnetically microactuated platforms for dynamic microphysiological systems
22. Interconnected organ models for skin and liver
23. Heart-Liver-Vascular Interconnected Microphysiological Systems
24. Rocking Platform Technology for Interconnected Organ Models
Microfluidic Cell Culture Systems, Second Edition, applies the design and experimental techniques used in MEMS microfabrication and microfluidics to cell culture systems. The book serves as a professional reference, providing a practical guide to the design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. It covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. This updated edition contains new material that strengthens the focus on in vitro models that are useful for drug discovery and development.
New sections in this edition review liver organ models from an industry perspective, while other new material highlights the development of organ models and systems for specific applications in disease modeling and drug safety.
- Provides design and operation methodology for microfluidic and microfabricated biomaterials approaches to tissue engineering and cell culture systems
- Comprehensively covers strategies and techniques, ranging from academic first principles, to industrial scale-up approaches
- Offers design principles, operation techniques and background information/perspectives
Academic scientists working in areas related to cell and tissue culture, industry scientists working on drug discovery, safety, and drug screening, and clinical researchers working toward regenerative medicine technologies
- No. of pages:
- © Elsevier 2018
- 1st June 2018
- eBook ISBN:
- Paperback ISBN:
Jeffrey T. Borenstein is Laboratory Technical Staff at the Charles Stark Draper Laboratory in Cambridge, Massachusetts, USA. Dr. Borenstein is a Technical Director for several of Draper’s programs in artificial organs, tissue engineering and implantable devices. His expertise is in MEMS fabrication technology, biological microsystems and the development of microdevices for therapeutic clinical applications. Dr. Borenstein currently serves as Principal Investigator for projects involving the application of microsystems technology towards engineered tissue constructs for organ assist devices and drug discovery, as well as implantable drug delivery systems for hearing loss and other diseases. These programs are funded by the Department of Defense, the National Institutes of Health and several commercial sponsors.
Laboratory Technical Staff, Charles Stark Draper Laboratory in Cambridge, Massachusetts, USA
Vishal Tandon is a Research Fellow at the Biomedical Engineering Center, Draper University, USA. His research focuses on the design and testing of implantable microfluidic devices for drug delivery into the ear.
Research Fellow, Biomedical Engineering Center, Draper University, Massachusetts, USA
Sarah Tao is Senior Manager, New Technologies at CooperVision, Inc. She was previously Senior Member Technical Staff, MEMS Design Group at Draper University, and Research Professor Equivalent, Bioengineering and Therapeutic Sciences at the University of California, San Francisco, USA. Her research interests lie in the areas of biomaterials, nanotechnology, regenerative medicine, drug delivery, BioMEMS, microfluidics and cell culture.
Senior Manager, New Technologies at CooperVision, Inc., California, USA
Dr. Charest is director of in vitro model and organ-assist work at Draper Laboratory. The work of his teams leverages micro- and nano-fabrication along with advanced machining techniques to create systems which recapitulate native tissue and organ architecture, morphology, and function in vitro. The systems span applications from medical devices to screening platforms for pharmaceuticals, and impact fields of use in various organ and tissue types such as tumor, kidney, vascular tissue and lung. Dr. Charest graduated from Georgia Tech with an MS and PhD in Mechanical Engineering and from Penn State with a BS in Mechanical Engineering.
Director, In vitro Model and Organ-assist Work, Draper Laboratory, Massachusetts, USA