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Microbial Toxins: Tools in Enzymology - 1st Edition - ISBN: 9780121820664, 9780080882826

Microbial Toxins: Tools in Enzymology, Volume 165

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: Sidney Harshman
eBook ISBN: 9780080882826
Imprint: Academic Press
Published Date: 28th October 1988
Page Count: 440


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Table of Contents

Preparation of Toxins: Gram-Positive Cocci: S. Harshman, N. Sugg, and P.Cassidy, Preparation and Purification of Staphylococcal Alpha Toxin. M. Plommet, Preparation and Purification of gg-Hemolysin of Staphylococci. T.H. Birkbeck and J.H. Freer, Purification and Assay of Staphylococcal ~gd-Lysin. M. Noda and I. Kato, Purification and Crystallization of Staphylococcal Leukocidin. J.C.S. de Azavedo, C.J. Bailey, and J.P. Arbuthnott, Purification of Epidermolytic Toxin of Staphylococcus aureus. D.A. Blomster-Hautamaa and P.M. Schlievert, Preparation of Toxic Shock Syndrome Toxin-1. J.J. Iandolo and R.K. Tweten, Purification of Staphylococcal Enterotoxins. J.E. Alouf and C. Geoffroy, Production, Purification, and Assay of Streptolysin O. J.E. Alouf and C. Loridan, Production, Purification, and Assay of Streptolysin S. C. Geoffroy and J.E. Alouf, Production, Purification, and Assay of Streptococcal Erythrogenic Toxin. Gram-Positive Bacilli: S.F. Carroll, J.T. Barbieri, and R.J. Collier, Diphtheria Toxin: Purification and Properties. L.L. Simpson, J.J. Schmidt, and J.L. Middlebrook, Isolation and Characterization of the Botulinum Neurotoxins. J.P. Robinson, Purification of Tetanus Toxin and Its Major Peptides. C. Jolivet-Reynaud, H. Moreau, and J.E. Alouf, Purification of Alpha Toxin from Clostridium perfringens: Phospholipase C. J.L. McDonel and B.A. McClane, Production, Purification, and Assay of Clostridium perfringens Enterotoxin. S.H. Leppla, Production and Purification of Anthrax Toxin. Gram-Negative Cocci: A.G. Plaut, Production and Isolation of Neissereal IgA1 Proteases. V.Y. Perera, Purification of Pertussis Toxin. Gram-Negative Bacilli: R.N. Greenberg and A.M.K. Saeed, Purification of Escherichia coli Heat-Stable Enterotoxin. G.A. Bohach, S.J. Cavalieri, and I.S. Snyder, Purification of Escherichia coli ~ga-Hemolysin. K.J. Kozak and C.B. Saelinger, Preparation of Pseudomonas Exotoxin A. G.T. Keusch, A. Donohue-Rolfe, M. Jacewitz, and A.V. Kane, Shigella Toxin: Production and Purification. T.C. Montie, Preparation of Yersinia pestis Plague Murine Toxin. Vibrios: J.J. Mekalanos, Production and Purification of Cholera Toxin. A.S. Kreger, M.H. Kothary, and L.D. Gray, Cytolytic Toxins of Vibrio vulnificus and Vibrio damsela. Y. Takeda, Thermostable Direct Hemolysin of Vibrio parahaemolyticus. J.T. Buckley and S.P. Howard, Aerolysin from Aeromonas hydrophila. akA. Ljungh and T. Wadstrvm, Preparation of Hemolysin from Aeromonas. Immunotoxins: T.N. Oeltmann and R.G. Wiley, Preparation of Diphtheria Toxin Fragment A Coupled to Hormone. Assay of Toxins: A.W. Bernheimer, Assay of Hemolytic Toxins. S.F. Carroll and R.J. Collier, Diphtheria Toxin: Quantification and Assay. C.B. Saelinger, Use of Exotoxin A to Inhibit Protein Synthesis. A. Dohohue-Rolfe, M. Jacewicz, and G.T. Keusch, Shigella Toxin as Inhibitor of Protein Synthesis. D.M. Gill and M. Woolkalis, [32P]ADP-Ribosylation of Proteins Catalyzed by Cholera Toxin and Related Heat-Labile Enterotoxins. M. Woolkalis, D.M. Gill, and J. Coburn, Assay and Purification of Cytosolic Factor Required for Cholera Toxin Activity. R.G. Wiley, A. Donohue-Rolfe, G.T. Keusch, and T.N. Oeltmann, Selective Destruction of Vagal Sensory Neurons Using Shigella Cytotoxin. J.J. Lipman, Measurement of Neurotoxic Actions on Mammalian Nerve Impulse Conduction. J.J. Lipman, Electroencephalogram as A Tool for Assaying Neurotoxicity. M. Thelestam, Assay of Pore-Forming Toxins in Cultured Cells Using Radioisotopes. R. F|ssle, J. Tranum-Jensen, A. Sziegoleit, and S. Bhakdi, Incorporation of Toxin Pores into Liposomes. C. Jolivet-Reynaud, H. Moreau, and J.E. Alouf, Assay Methods for Alpha Toxin from Clostridium perfringens: Phospholipase C. L.W. Daniel, L. King, and M. Kennedy, Phospholipase Activity of Bacterial Toxins. G.A. Bohach and P.M. Schlievert, Detection of Endotoxin by Enhancement with Toxic Shock Syndrome Toxin-1 (TSST-1). M.S. Bergdoll, Ileal Loop Fluid Accumulation Test for Diarrheal Toxins. M.S. Bergdoll, Monkey Feeding Test for Staphyloccocal Enterotoxin. J.C.S. de Azavedo and J.P. Arbuthnott, Assays for Epidermolytic Toxin of Staphylococcus aureus. P.M. Schlievert, Immunochemical Assays for Toxic Shock Syndrome Toxin-1. J.C.S. de Azavedo, R.N. Lucken, and J.P. Arbuthnott, Chick Embryo Assay for Staphylococcal Toxic Shock Syndrome Toxin-1. C. Montecucco, Photoreactive Lipids for the Study of Membrane-Penetrating Toxins. J. Tranum-Jensen, Electron Microscopy: Assays Involving Negative Staining. J. Tranum-Jensen, Electron Microscopy: Assays Involving Freeze-Fracture and Freeze-Etching. J. Reidler and J.P. Robinson, Two-Dimensional Crystals of Tetanus Toxin. Addenda. Author Index. Subject Index.


Microbial toxins are useful as agents in studies on membrane structure, biochemical pathways, and cellular regulatory mechanisms. Since relatively few of these toxins are commercially available, investigators must prepare their own reagents. This book provides in a single source the methods for preparing specific microbial toxins and their assay. It demonstrates their usefulness as reagents in biochemical and physiological studies, particularly at the cellular and molecular levels.


Biochemists, microbiologists, physiologists, molecular biologists, cell biologists, analytical chemists, pharmacologists, toxicologists


No. of pages:
© Academic Press 1988
28th October 1988
Academic Press
eBook ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

Sidney Harshman

Affiliations and Expertise

Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.