Microbial Toxins: Tools in Enzymology, Volume 165

1st Edition

Serial Volume Editors: Sidney Harshman
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121820664
eBook ISBN: 9780080882826
Imprint: Academic Press
Published Date: 28th October 1988
Page Count: 440


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Table of Contents

Preparation of Toxins: Gram-Positive Cocci:

S. Harshman, N. Sugg, and P.Cassidy, Preparation and Purification of Staphylococcal Alpha Toxin.

M. Plommet, Preparation and Purification of ~gg-Hemolysin of Staphylococci.

T.H. Birkbeck and J.H. Freer, Purification and Assay of Staphylococcal ~gd-Lysin.

M. Noda and I. Kato, Purification and Crystallization of Staphylococcal Leukocidin.

J.C.S. de Azavedo, C.J. Bailey, and J.P. Arbuthnott, Purification of Epidermolytic Toxin of Staphylococcus aureus.

D.A. Blomster-Hautamaa and P.M. Schlievert, Preparation of Toxic Shock Syndrome Toxin-1.

J.J. Iandolo and R.K. Tweten, Purification of Staphylococcal Enterotoxins.

J.E. Alouf and C. Geoffroy, Production, Purification, and Assay of Streptolysin O.

J.E. Alouf and C. Loridan, Production, Purification, and Assay of Streptolysin S.

C. Geoffroy and J.E. Alouf, Production, Purification, and Assay of Streptococcal Erythrogenic Toxin.

Gram-Positive Bacilli:

S.F. Carroll, J.T. Barbieri, and R.J. Collier, Diphtheria Toxin: Purification and Properties.

L.L. Simpson, J.J. Schmidt, and J.L. Middlebrook, Isolation and Characterization of the Botulinum Neurotoxins.

J.P. Robinson, Purification of Tetanus Toxin and Its Major Peptides.

C. Jolivet-Reynaud, H. Moreau, and J.E. Alouf, Purification of Alpha Toxin from Clostridium perfringens: Phospholipase C.

J.L. McDonel and B.A. McClane, Production, Purification, and Assay of Clostridium perfringens Enterotoxin.

S.H. Leppla, Production and Purification of Anthrax Toxin.

Gram-Negative Cocci:

A.G. Plaut, Pr


Microbial toxins are useful as agents in studies on membrane structure, biochemical pathways, and cellular regulatory mechanisms. Since relatively few of these toxins are commercially available, investigators must prepare their own reagents. This book provides in a single source the methods for preparing specific microbial toxins and their assay. It demonstrates their usefulness as reagents in biochemical and physiological studies, particularly at the cellular and molecular levels.


Biochemists, microbiologists, physiologists, molecular biologists, cell biologists, analytical chemists, pharmacologists, toxicologists


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© Academic Press 1988
Academic Press
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About the Serial Volume Editors

Sidney Harshman Serial Volume Editor

Affiliations and Expertise

Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA