Description

Microbial toxins are useful as agents in studies on membrane structure, biochemical pathways, and cellular regulatory mechanisms. Since relatively few of these toxins are commercially available, investigators must prepare their own reagents. This book provides in a single source the methods for preparing specific microbial toxins and their assay. It demonstrates their usefulness as reagents in biochemical and physiological studies, particularly at the cellular and molecular levels.

Readership

Biochemists, microbiologists, physiologists, molecular biologists, cell biologists, analytical chemists, pharmacologists, toxicologists

Table of Contents

Preparation of Toxins: Gram-Positive Cocci: S. Harshman, N. Sugg, and P.Cassidy, Preparation and Purification of Staphylococcal Alpha Toxin. M. Plommet, Preparation and Purification of ~gg-Hemolysin of Staphylococci. T.H. Birkbeck and J.H. Freer, Purification and Assay of Staphylococcal ~gd-Lysin. M. Noda and I. Kato, Purification and Crystallization of Staphylococcal Leukocidin. J.C.S. de Azavedo, C.J. Bailey, and J.P. Arbuthnott, Purification of Epidermolytic Toxin of Staphylococcus aureus. D.A. Blomster-Hautamaa and P.M. Schlievert, Preparation of Toxic Shock Syndrome Toxin-1. J.J. Iandolo and R.K. Tweten, Purification of Staphylococcal Enterotoxins. J.E. Alouf and C. Geoffroy, Production, Purification, and Assay of Streptolysin O. J.E. Alouf and C. Loridan, Production, Purification, and Assay of Streptolysin S. C. Geoffroy and J.E. Alouf, Production, Purification, and Assay of Streptococcal Erythrogenic Toxin. Gram-Positive Bacilli: S.F. Carroll, J.T. Barbieri, and R.J. Collier, Diphtheria Toxin: Purification and Properties. L.L. Simpson, J.J. Schmidt, and J.L. Middlebrook, Isolation and Characterization of the Botulinum Neurotoxins. J.P. Robinson, Purification of Tetanus Toxin and Its Major Peptides. C. Jolivet-Reynaud, H. Moreau, and J.E. Alouf, Purification of Alpha Toxin from Clostridium perfringens: Phospholipase C. J.L. McDonel and B.A. McClane, Production, Purification, and Assay of Clostridium perfringens Enterotoxin. S.H. Leppla, Production and Purification of Anthrax Toxin. Gram-Negative Cocci: A.G. Plaut, Pr

Details

No. of pages:
440
Language:
English
Copyright:
© 1988
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080882826
Print ISBN:
9780121820664

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Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY