Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers microbial metagenomics, metatranscriptomics, and metaproteomics, and includes chapters on such topics as in-solution FISH for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers microbial metagenomics, metatranscriptomics, and metaproteomics
  • Contains chapters on such topics as in-solution fluorescence in situ hybridization (FISH) for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

Series Page

METHODS IN ENZYMOLOGY

Contributors

Preface

Methods in Enzymology

Section I: Preparation and Genomic Analyses of Single Cells from Natural Populations

Chapter One. In-Solution Fluorescence In Situ Hybridization and Fluorescence-Activated Cell Sorting for Single Cell and Population Genome Recovery

Abstract

1 Introduction

2 Technical Protocols and Considerations

3 Case Study: Targeted Isolation of Anaerobic Methanotrophic Archaea for Single Cell and Population Genomics

4 Summary

Acknowledgments

References

Chapter Two. Whole Cell Immunomagnetic Enrichment of Environmental Microbial Consortia Using rRNA-Targeted Magneto-FISH

Abstract

1 Introduction

2 Methods

3 Results and Discussion

4 Summary

Acknowledgments

References

Chapter Three. Coupling FACS and Genomic Methods for the Characterization of Uncultivated Symbionts

Abstract

1 Introduction

2 Fluorescence-Activated Cell Sorting

3 Metagenomic Sequencing of Uncultured Symbiont Populations

4 Determining Host Identity and Metabolism

References

Chapter Four. Optofluidic Cell Selection from Complex Microbial Communities for Single-Genome Analysis

Abstract

1 Introduction

2 Optical Tweezing for Cell Isolation

3 Optical Hardware Setup

4 Microfluidics Configuration and Setup

5 Procedure for Sorting and Amplifying Single Cells

6 Software

7 Performance and Limitations

Acknowledgments

References

Chapter Five. Quantifying and Identifying the Active and Damaged Subsets of Indigenous Microbial Communities

Abstract

1 Introduction

2 Flow Cytometry and Fluorescent Dyes

3 Experimental Procedures

4 Experimental Validation

5 Troubleshooting

Acknowled

Details

No. of pages:
688
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
eBook ISBN:
9780124079274
Print ISBN:
9780124078635

About the serial-volume-editor