Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular parasitism. Each chapter outlines practical procedures and describes the rationale behind their development. This volume should prove useful to anyone interested in the biology of infectious agents, or their exploitation as a new generation of cell biological reagents.

Key Features

@introbul:Key Features @bul:* Introduction by renowned microbiologist Dr. Stanley Falkow * Covers manipulation of pathogens, especially generation and selection of non-virulent phenotypes * Guides researchers in the study of intracellular pathogenesis * Describes microbial adherence and phagocytosis assays * Focuses on protein trafficking in infected cells * Well-illustrated with color plates, halftones, and diagrams


Researchers, students, and technicians in cell and developmental biology, microbiology, parasitology, immunology, biochemistry, pathology, genetics, and molecular biology.

Table of Contents

D.G. Russell, Preface. S. Falkow, Introduction. D.G. Russell, Obtaining and Maintaining Microbial Pathogens. Manipulation of Pathogens: W. Trager, Cultivation of Malaria Parasites. D.S. Roos, R.G.K. Donald, N.S. Morrissette, and A.L.C. Moulton, Molecular Tools for Genetic Dissection of the Protozoan Parasite Toxoplasma gondii. J.H. LeBowitz, Transfection Experiments with Leishmania. R. Tsolis and F. Heffron, Mutagenesis and Variant Selection in Salmonella. N.D. Connell, Mycobacterium: Isolation, Maintenance, Transformation, and Mutant Selection. Microbial Adherence and Invasion Assays: G.J. Bancroft, H.L. Collins, L.B. Sigola, and C.E. Cross, Modulation of Murine Macrophage Behavior in Vivo and in Vitro. E.J. Brown, In Vitro Assays of Phagocytic Function of Human Peripheral Blood Leukocytes: Receptor Modulation and Signal Transduction. P. Falk, T. Borén, D. Haslam, and M. Caparon, Bacterial Adhesion and Colonization Assays. I. Crandall and I.W. Sherman, Cytoadherence and the Plasmodium falciparum-Infected Erythrocyte. The Study of Intracellular Pathogenesis: M.J. Blackman, Purification of Plasmodium falciparum Merozoites for Analysis of the Processing of Merozoite Surface Protein-1. K. Haldar, H.G. Elmendorf, A. Das, W.L. Li, D.J.P. Ferguson, and B.C. Elford, In Vitro Secretory Assays with Erythrocyte-Free Malaria Parasites. K.H. Berger and R.R. Isberg, Intracellular Survival by Legionella. P. Chakraborty, S. Sturgill-Koszycki, and D.G. Russell, Isolation and Characteriz


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© 1994
Academic Press
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About the serial-volume-editor

David Russell

Affiliations and Expertise

Washington University School of Medicine, St. Louis, Missouri, U.S.A.


@qu:"Microbes as Tools for Cell Biology contains a potpourri of techniques." @source:--TRENDS IN MICROBIOLOGY