Microbeam Analysis in Biology

Microbeam Analysis in Biology

1st Edition - January 1, 1979

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  • Editor: Claude Lechene
  • eBook ISBN: 9780323143349

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Description

Microbeam Analysis in Biology contains the proceedings of a workshop on Biological X-Ray Microanalysis by Electron Beam Excitation, held in Boston, Massachusetts on August 25-26, 1977. This book focuses on the principles, techniques, and biological use of electron probe microanalysis, energy-loss spectroscopy, and ion probe microanalysis. This text reflects the emphasis of the workshop on presenting the principles of analysis, the optimization of operating conditions, the description of successful techniques for sample preparation and quantitation, the illustration of problems and pitfalls, and the direction of microbeam analysis in biology.

Table of Contents


  • Speakers

    Participants

    Preface

    I. Instrumentation: X-ray, Mass, and Electron Energy Loss Spectroscopy

    Analytical Transmission Electron Microscopy in Biology

    Factors Affecting X-Ray Sensitivity in Electron Microbeam Instrumentation

    X-Ray Spectral Artifacts Encountered in the SEM and STEM

    Electron Energy Loss Spectroscopy (ELS) Within the Electron Microscope. Where Are We?

    Quantitation and Detection Limits in Electron Energy Loss Spectroscopy (EELS) of Thin Biological Sections

    A Practical Electron Energy Loss Spectrometer

    Electron Energy Loss Microanalysis of Biological Material

    Discussion of ELS

    Secondary Ion Emission Microanalysis: Biomedical Applications

    Secondary-Ion Mass Spectrometry: Future Application to Biological Samples

    General Discussion

    II. Quantitation

    Quantitative Microprobe Analysis in Biology

    Statistics and X-Ray Analysis

    Problems of the Continuum-Normalization Method for the Quantitative Analysis of Sections of Soft Tissue

    Electron Probe Microanalysis of Thin Samples

    A Catalogue of Artifacts Observed in Energy-Dispersive X-Ray Spectrometry and Their Influence on Analysis

    Quantitative Electron Probe Analysis of Biological Thin Sections

    III. Liquid-Droplet and Single-Cell Analysis

    Electron Probe Analysis of Liquid Droplets

    X-Ray Microanalysis of Red Blood Cells

    Sodium and Potassium Microprobe Analysis of Isolated Human Red Blood Cells or Red Blood Cell Resealed Ghosts: A Comparison of Results Between Freeze-Dried or Frozen-Hydrated Cells

    Energy-Dispersive X-Ray Analysis of Biological Microdroplets

    Application of the Electron Microprobe to Analysis of Developing Frog Oocytes

    IV. Analysis of Frozen-Hydrated Tissue

    Electron Probe Microanalysis of Frozen-Hydrated Bulk Specimens: Basic Experiments

    The Electron Microprobe X-Ray Analysis of Frozen-Hydrated Sections with New Information on Fluid Transporting Epithelia

    Electron Probe Analysis of Frozen-Hydrated Bulk Tissue

    Freezing of Muscle Fibers, Ultrathin Cryocutting, and Transfer of Frozen-Hydrated Sections

    V . Analysis of thin and Ultrathin Preparations: I. Nondiffusible Elements

    Electron Probe Microanalysis: The Study of Nondiffusible Elements on Ultrathin Sections

    X-Ray Microanalysis of Diffusible and Nondiffusible Elements in Ultrathin Biological Tissues Using Human Sperm Cells as a Model for Investigating Specimen Preparation

    Cytochemical/Analytical Studies of Biogenic Amines in Ultrathin Preparations

    Renal Na-K-ATPase: Quantitative X-Ray Microanalysis

    VI. Analysis of thin and Ultrathin Preparations: II. Diffusible Elements

    Electron Probe X-Ray Microanalysis and the Cellular Basis of Transepithelial Calcium Transport

    Quantitative Determination of Cellular Electrolyte Concentrations in Thin Freeze-Dried Cryosections Using Energy-Dispersive X-Ray Microanalysis

    Cryoultramicrotomy for Electron Probe Analysis

    Mitochondrial Calcium Content in Vascular Smooth Muscle

    Quantitative Electron Probe Microanalysis of Ultrathin Biological Sections

    Use of Beryllium and Graphite-Polymer Substrates to Reduce Spurious X-Ray Signal

    Problems and Progress in Quantitative Localization of Sodium, Potassium and Chlorine in Freeze-Dried Sections: Use of Erythrocytes as a Model System

    A Study of the Maintenance of Calcium and Phosphorus in Anhydrous Preparations of Mineralized Tissue for Electron Optical Examination

    Electron Probe Measurement of Electrolytes in Rat Foot Muscle

    Index

Product details

  • No. of pages: 694
  • Language: English
  • Copyright: © Academic Press 1979
  • Published: January 1, 1979
  • Imprint: Academic Press
  • eBook ISBN: 9780323143349

About the Editor

Claude Lechene

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