Description

This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.

Key Features

  • The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications
  • Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues
  • The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels

Readership

Investigators and students whose research interests include the development, physiology, or pathophysiology of adipose tissues

Table of Contents

Contributors
Preface
Chapter One: Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice
Abstract

1 Introduction

2 EMSC Isolation, Culture, Cryopreservation, and Differentiation

3 Conclusions

Acknowledgments

Chapter Two: 3-D Adipocyte Differentiation and Peri-adipocyte Collagen Turnover
Abstract

1 Introduction

2 Materials and Methods

Acknowledgment

Chapter Three: Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells
Abstract

1 Introduction

2 Human Pluripotent Stem Cells

3 Differentiation into Mesenchymal Progenitor Cells

4 Differentiation into Adipocytes

Chapter Four: Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells
Abstract

1 Introduction

2 Isolation of ASCs from AT

3 Protocols for Proliferation, Subculture, and Freezing Down

4 Protocols to Differentiate Human ASCs to Adipocytes

5 Methods to Use Newly Differentiated Adipocytes for Metabolic Studies

Acknowledgments

Chapter Five: Human Adipose-Derived Stromal/Stem Cell Isolation, Culture, and Osteogenic Differentiation
Abstract

1 Introduction

2 Methods for Cell Culture

3 Methods for Scaffold Preparation

4 Material Characterization

5 Cell Loading and Scaffold Culture

6 Cell Viability and Proliferation

7 Assessing Osteogenic Differentiation

Chapter Six: Analysis of Adipose Tissue Lipid Using Mass Spectrometry
Abstract

1 Introduction

2 Neutral Lipids

3 Phospholipids

4 Summary

Acknowledgments

Chapter Seven: Measurement of Long-Chain Fatty Acid Uptake into Adipocytes
Abstract

1 Introduction

2 Adipocyte Sources

3 Uptake Assay with Radioactive Fatty Acids

4 Uptake Assay with Flow Cytometer

Details

No. of pages:
344
Language:
English
Copyright:
© 2014
Published:
Imprint:
Academic Press
Electronic ISBN:
9780128003367
Print ISBN:
9780128002803

About the serial-volume-editor

Ormond Macdougald

Ormond A. MacDougald, Ph.D. is the John A. Faulkner Collegiate Professor in Physiology, and a Professor of Molecular & Integrative Physiology, and Internal Medicine in the University of Michigan Medical School. He currently is a Fulbright Scholar at Pembroke College and the Institute of Metabolic Science, University of Cambridge. MacDougald is an internationally recognized investigator for his work on adipocyte differentiation and metabolism. Specifically, his research explores the signals that act on mesenchymal stem cells to influence fat tissue development and metabolism, including effects on insulin sensitivity. This research provides important insight into the problems of obesity and type 2 diabetes. MacDougald’s bibliography reflects more than 100 peer-reviewed journal articles, reviews, and book chapters. MacDougald serves on numerous editorial boards and provides extensive peer-review service for journals and funding agencies. He’s a highly sought-after speaker at national and international meetings. In 2005, MacDougald received the UM Medical School’s Achievement in Basic Science Research Award. The same year, he earned the Henry Pickering Bowditch Award, one of the American Physiological Society’s highest honors, given to “a distinguished young physiologist less than 42 years of age who has made original and outstanding contributions in physiology.” More recently he was elected Fellow of the American Association for the Advancement of Science. In addition to his research accomplishments, MacDougald is highly regarded for his dedication and commitment to education. He has served in numerous educational roles as director, co-director and lecturer in departmental courses, and has been a member of more than 80 preliminary examination and graduate dissertation committees. He served as director of the Molecular & Integrative Physiology graduate program, and he initiated and directs a summer research program for undergraduate students. He received the Rack