Description

This book is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers that are world leaders in this field. Detailed lab protocols range from methods to visualize adipocytes and adipose tissues in humans and experimental models, to convert stem cells into white and brown adipocytes in vitro, to evaluate aspects of adipocyte metabolism, to inducibly knock out genes in adipose tissues, and to evaluate transcriptional control of adipogenesis on a global scale.

Key Features

  • The study of adipose tissue goes hand in hand with our global effort to understand and reverse the epidemic of obesity and associated medical complications
  • Contributors include leading researchers who have made tremendous contributions to our ability to investigate white and brown adipose tissues
  • The wide variety of experimental approaches detailed within this volume: including the evaluation of adipose tissue biology at the molecular, biochemical, cellular, tissue, and organismal levels

Readership

Investigators and students whose research interests include the development, physiology, or pathophysiology of adipose tissues

Table of Contents

Contributors
Preface
Chapter One: Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice
Abstract

1 Introduction

2 EMSC Isolation, Culture, Cryopreservation, and Differentiation

3 Conclusions

Acknowledgments

Chapter Two: 3-D Adipocyte Differentiation and Peri-adipocyte Collagen Turnover
Abstract

1 Introduction

2 Materials and Methods

Acknowledgment

Chapter Three: Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells
Abstract

1 Introduction

2 Human Pluripotent Stem Cells

3 Differentiation into Mesenchymal Progenitor Cells

4 Differentiation into Adipocytes

Chapter Four: Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells
Abstract

1 Introduction

2 Isolation of ASCs from AT

3 Protocols for Proliferation, Subculture, and Freezing Down

4 Protocols to Differentiate Human ASCs to Adipocytes

5 Methods to Use Newly Differentiated Adipocytes for Metabolic Studies

Acknowledgments

Chapter Five: Human Adipose-Derived Stromal/Stem Cell Isolation, Culture, and Osteogenic Differentiation
Abstract

1 Introduction

2 Methods for Cell Culture

3 Methods for Scaffold Preparation

4 Material Characterization

5 Cell Loading and Scaffold Culture

6 Cell Viability and Proliferation

7 Assessing Osteogenic Differentiation

Chapter Six: Analysis of Adipose Tissue Lipid Using Mass Spectrometry
Abstract

1 Introduction

2 Neutral Lipids

3 Phospholipids

4 Summary

Acknowledgments

Chapter Seven: Measurement of Long-Chain Fatty Acid Uptake into Adipocytes
Abstract

1 Introduction

2 Adipocyte Sources

3 Uptake Assay with Radioactive Fatty Acids

4 Uptake Assay with Flow Cytometer

Details

No. of pages:
344
Language:
English
Copyright:
© 2014
Published:
Imprint:
Academic Press
Print ISBN:
9780128002803
Electronic ISBN:
9780128003367