Methods in Virology

Methods in Virology

Volume III

1st Edition - January 1, 1967

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  • Editors: Karl Maramorosch, Hilary Koprowski
  • eBook ISBN: 9781483262260

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Methods in Virology, Volume III focuses on the advancements of methods employed in virology, including immunological, microscopic, and serological techniques and transformation assays. The selection first offers information on the analysis of protein constituents and lipid components of viruses. Discussions focus on the applications of the existing methodology to lipid-containing viruses; physical methods for the characterization of virus proteins; renaturation of virus proteins and reconstitution of viruses; and chemical methods for the characterization of virus proteins. The text then elaborates on RNA polymerase, immunological techniques for animal viruses, and serological techniques for plant viruses. The book tackles the plaque assay of animal viruses, transformation assays, and the methods for selecting RNA bacteriophage. Topics include identification of the nucleic acid, assay methods for particular viruses, general consideration of the plaque assay method, virus-dilution media and procedures, monolayer assay methods, and incubation and staining of plates and counting of plaques. The manuscript also takes a look at the structural studies of viruses, microscopic techniques, electron microscopy of isolated virus particles and their components, and the application of thin sectioning. The selection is a vital source of data for researchers interested in the methods employed in virology.

Table of Contents

  • List of Contributors


    Contents of Other Volumes

    Chapter 1—Analysis of Protein Constituents of Viruses

    I. Introduction

    II. Chemical Methods for Characterization of Virus Proteins

    III. Physical Methods for Characterization of Virus Proteins

    IV. Amino Acid-Sequence Analysis of Virus Proteins

    V. Renaturation of Virus Proteins and Reconstitution of Viruses


    Chapter 2—Analysis of Lipid Components of Viruses

    I. Introduction

    II. Lipid Methodology

    III. Applications of Existing Methodology to Lipid-Containing Viruses

    IV. Summary


    Chapter 3—RNA Virus RNA Polymerase: Detection, Purification, and Properties

    I. Introduction

    II. Detection

    III. Purification

    IV. Properties of the Qβ-RNA Polymerase



    Chapter 4—Immunological Techniques for Animal Viruses

    I. Introduction

    II. Neutralization Test

    III. Hemagglutination (HA) and Hemagglutination-Inhibition (HI) Tests

    IV. Complement-Fixation (CF) Test

    V. Fluorescent-Antibody (FA) Techniques

    VI. Immunodiffusion

    VII. Other Tests


    Chapter 5—Serological Techniques for Plant Viruses

    I. The Preparation of Reagents

    II. The Precipitation Reaction

    III. Modifications of the Precipitation Reaction

    IV. Other Types of Serological Tests

    V. The Quantitative Estimation of Viruses

    VI. The Estimation of Antibody Concentration

    VII. Methods for Determining Serological Relationships Between Plant Viruses

    VIII. Labeled Antibody Methods


    Chapter 6—The Plaque Assay of Animal Viruses

    I. Introduction

    II. General Consideration of the Plaque Assay Method

    III. Preparation of Media

    IV. Preparation of Cell Suspensions

    V. Virus-Dilution Media and Procedures

    VI. Culture Containers

    VII. Monolayer Assay Methods

    VIII. Agar Cell-Suspension Methods

    IX. Incubation and Staining of Plates and Counting of Plaques

    X. Special Uses of the Plaque Assay Method

    XI. Some Recent Developments

    XII. Methods for Individual Viruses

    XIII. Some Sources of Difficulty


    Chapter 7—Transformation Assays

    I. Introduction

    II. Principles of Assays for Transforming Activity

    III. Assay Methods for Particular Viruses


    Chapter 8—Methods for Selecting RNA Bacteriophage

    I. Introduction

    II. General Techniques

    III. Selection Techniques

    IV. Identification of the Nucleic Acid


    Chapter 9—Structural Studies of Viruses

    I. The Scope of the Article

    II. Principles of Design of Viruses

    III. Interpretation of Electron Micrographs

    IV. Introduction to the Theory of Diffraction

    V. X-ray Diffraction from Virus Crystals

    VI. Low-Angle X-ray Diffraction in Solution

    VII. X-ray Diffraction from Orientated Rod Viruses

    VIII. Comparison of X-ray and Electron Microscope Techniques


    Chapter 10—Microscopic Techniques

    I. Fluorescent-Antibody Techniques

    II. Staining with Acridine Orange



    Chapter 11—Electron Microscopy of Isolated VirusParticles and Their Components

    I. Some General Remarks on the Application of the Electron Microscope to the Study of Biological Structure at the Macromolecular Level

    II. Specimen-Support Films

    III. Mounting of Isolated Virus Particles and Components

    IV. Shadow-Casting Techniques

    V. Replica Techniques

    VI. Positive Staining of Virus Particles and Their Components

    VII. The Application of Negative Staining Techniques to the Study of Virus Structure

    VIII. Particle Counting

    IX. Calibration of the Electron Microscope

    X. Conclusion


    Chapter 12—The Application of Thin Sectioning

    I. Introduction

    II. History

    III. Fixation

    IV. Embedding

    V. Sectioning

    VI. Specimen Grids and Supporting Film

    VII. Staining

    VIII. The Electron Microscope

    IX. Ancillary Techniques

    X. Conclusion

    XI. Illustrations


    Chapter 13—Autoradiographic Methods for Electron Microscopy

    I. Specimen

    II. The Emulsions

    III. Description of the Technique

    IV. Quantitative Aspects of Electron Autoradiography

    V. Advantages and Limitations of This Method


    Author Index

    Subject Index

Product details

  • No. of pages: 694
  • Language: English
  • Copyright: © Academic Press 1967
  • Published: January 1, 1967
  • Imprint: Academic Press
  • eBook ISBN: 9781483262260

About the Editors

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

Hilary Koprowski

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