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Methods in Virology, Volume VIII focuses on the methods used in virology, including microscopy, hybridization, viruses, and fingerprint analysis. The selection first offers information on the hybridization of viral nucleic acids; applications of oligonucleotide fingerprinting to the identification of viruses; and immunosorbent electron microscopy in plant virus studies. Discussions focus on the detection of double-stranded RNA, principles and mechanisms of fingerprint analysis, preparation of labeled nucleic acid probes, and basic methods of nucleic acid hybridization. The text then elaborates on quantitative transmission electron microscopy for the determination of mass-molecular weight of viruses and use of thin sectioning for visualization and identification of plant viruses. Topics include technical procedures for processing plant tissues, cytological modifications of diagnostic value, procedure and treatment of data to obtain the average mass of virus particles, and applications in virology. The book takes a look at the detection of genome-linked proteins of plant and animal viruses; methods for assay, purification, and characterization of prions; and the use of mosquitoes to detect and propagate viruses. The selection is a valuable source of information for researchers interested in the methods employed in virology.
Contents of Previous Volumes
Chapter 1. Hybridization of Viral Nucleic Acids: Newer Methods on Solid Media and in Solution
II. Purification of Nucleic Acid
III. Preparation of Labeled Nucleic Acid Probes
IV. Basic Methods of Nucleic Acid Hybridization
V. Southern Blot Hybridization
VI. Northern Blots
Chapter 2. Applications of Oligonucleotide Fingerprinting to the Identification of Viruses
II. Principles and Mechanisms of Fingerprint Analysis
III. Experimental Procedures
V. Summary and Conclusions
Chapter 3. Immunosorbent Electron Microscopy in Plant Virus Studies
II. Parameters of the Method
III. Varying Success with ISEM
V. Detection of Double-Stranded RNA
VI. Preparation of Grids for Later Use
VII. Problems and Prospects for the Future
Chapter 4. Quantitative Transmission Electron Microscopy for the Determination of Mass-Molecular Weight of Viruses
III. Procedure and Treatment of Data to Obtain the Average Dry Mass of Virus Particles
IV. Applications in Virology
Chapter 5. Use of Thin Sectioning for Visualization and Identification of Plant Viruses
II. Technical Procedures for Processing Plant Tissues
III. Cytological Modifications of Diagnostic Value
Chapter 6. Purification and Immunological Analyses of Plant Viral Inclusion Bodies
II. Inclusion Purification
Chapter 7. Use of Mosquitoes to Detect and Propagate Viruses
I. Rationale for Use of the Technique
II. Technical Considerations
III. Possible Limitations and Pitfalls
Chapter 8. Prions: Methods for Assay, Purification, and Characterization
II. Assays for Scrapie Prions
V. Identification and Detection of PrP
VI. Electron Microscopy
VII. Polarization Microscopy
VIII. Biocontainment and Decontamination Procedures
Chapter 9. Detection of Genome-Linked Proteins of Plant and Animal Viruses
II. Detection of a Genome-Linked Protein (VPg)
III. Characterization of Genome-Linked Proteins and Adjacent Nucleotide Sequences
IV. Biosynthesis of Genome-Linked Proteins
V. Possible Functions of Genome-Linked Proteins
- No. of pages:
- © Academic Press 1984
- 7th December 1984
- Academic Press
- eBook ISBN:
Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.
Rutgers University, New Brunswick, NJ, USA
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