Methods in Virology - 1st Edition - ISBN: 9780124702080, 9781483259017

Methods in Virology

1st Edition

Volume VIII

Editors: Karl Maramorosch Hilary Koprowski
eBook ISBN: 9781483259017
Imprint: Academic Press
Published Date: 7th December 1984
Page Count: 414
Sales tax will be calculated at check-out Price includes VAT/GST
43.99
54.95
72.95
Unavailable
Price includes VAT/GST
× DRM-Free

Easy - Download and start reading immediately. There’s no activation process to access eBooks; all eBooks are fully searchable, and enabled for copying, pasting, and printing.

Flexible - Read on multiple operating systems and devices. Easily read eBooks on smart phones, computers, or any eBook readers, including Kindle.

Open - Buy once, receive and download all available eBook formats, including PDF, EPUB, and Mobi (for Kindle).

Institutional Access

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Description

Methods in Virology, Volume VIII focuses on the methods used in virology, including microscopy, hybridization, viruses, and fingerprint analysis.

The selection first offers information on the hybridization of viral nucleic acids; applications of oligonucleotide fingerprinting to the identification of viruses; and immunosorbent electron microscopy in plant virus studies. Discussions focus on the detection of double-stranded RNA, principles and mechanisms of fingerprint analysis, preparation of labeled nucleic acid probes, and basic methods of nucleic acid hybridization. The text then elaborates on quantitative transmission electron microscopy for the determination of mass-molecular weight of viruses and use of thin sectioning for visualization and identification of plant viruses. Topics include technical procedures for processing plant tissues, cytological modifications of diagnostic value, procedure and treatment of data to obtain the average mass of virus particles, and applications in virology. The book takes a look at the detection of genome-linked proteins of plant and animal viruses; methods for assay, purification, and characterization of prions; and the use of mosquitoes to detect and propagate viruses.

The selection is a valuable source of information for researchers interested in the methods employed in virology.

Table of Contents


Contributors


Preface


Contents of Previous Volumes


Chapter 1. Hybridization of Viral Nucleic Acids: Newer Methods on Solid Media and in Solution


I. Introduction


II. Purification of Nucleic Acid


III. Preparation of Labeled Nucleic Acid Probes


IV. Basic Methods of Nucleic Acid Hybridization


V. Southern Blot Hybridization


VI. Northern Blots


References


Chapter 2. Applications of Oligonucleotide Fingerprinting to the Identification of Viruses


I. Introduction


II. Principles and Mechanisms of Fingerprint Analysis


III. Experimental Procedures


IV. Applications


V. Summary and Conclusions


References


Chapter 3. Immunosorbent Electron Microscopy in Plant Virus Studies


I. Introduction


II. Parameters of the Method


III. Varying Success with ISEM


IV. Applications


V. Detection of Double-Stranded RNA


VI. Preparation of Grids for Later Use


VII. Problems and Prospects for the Future


References


Chapter 4. Quantitative Transmission Electron Microscopy for the Determination of Mass-Molecular Weight of Viruses


I. Introduction


II. Theory


III. Procedure and Treatment of Data to Obtain the Average Dry Mass of Virus Particles


IV. Applications in Virology


V. Discussion


VI. Summary


References


Chapter 5. Use of Thin Sectioning for Visualization and Identification of Plant Viruses


I. Introduction


II. Technical Procedures for Processing Plant Tissues


III. Cytological Modifications of Diagnostic Value


References


Chapter 6. Purification and Immunological Analyses of Plant Viral Inclusion

Details

No. of pages:
414
Language:
English
Copyright:
© Academic Press 1984
Published:
Imprint:
Academic Press
eBook ISBN:
9781483259017

About the Editor

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

Hilary Koprowski

Ratings and Reviews