Methods in Virology

Methods in Virology

Volume VIII

1st Edition - December 7, 1984

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  • Editors: Karl Maramorosch, Hilary Koprowski
  • eBook ISBN: 9781483259017

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Description

Methods in Virology, Volume VIII focuses on the methods used in virology, including microscopy, hybridization, viruses, and fingerprint analysis. The selection first offers information on the hybridization of viral nucleic acids; applications of oligonucleotide fingerprinting to the identification of viruses; and immunosorbent electron microscopy in plant virus studies. Discussions focus on the detection of double-stranded RNA, principles and mechanisms of fingerprint analysis, preparation of labeled nucleic acid probes, and basic methods of nucleic acid hybridization. The text then elaborates on quantitative transmission electron microscopy for the determination of mass-molecular weight of viruses and use of thin sectioning for visualization and identification of plant viruses. Topics include technical procedures for processing plant tissues, cytological modifications of diagnostic value, procedure and treatment of data to obtain the average mass of virus particles, and applications in virology. The book takes a look at the detection of genome-linked proteins of plant and animal viruses; methods for assay, purification, and characterization of prions; and the use of mosquitoes to detect and propagate viruses. The selection is a valuable source of information for researchers interested in the methods employed in virology.

Table of Contents


  • Contributors

    Preface

    Contents of Previous Volumes

    Chapter 1. Hybridization of Viral Nucleic Acids: Newer Methods on Solid Media and in Solution

    I. Introduction

    II. Purification of Nucleic Acid

    III. Preparation of Labeled Nucleic Acid Probes

    IV. Basic Methods of Nucleic Acid Hybridization

    V. Southern Blot Hybridization

    VI. Northern Blots

    References

    Chapter 2. Applications of Oligonucleotide Fingerprinting to the Identification of Viruses

    I. Introduction

    II. Principles and Mechanisms of Fingerprint Analysis

    III. Experimental Procedures

    IV. Applications

    V. Summary and Conclusions

    References

    Chapter 3. Immunosorbent Electron Microscopy in Plant Virus Studies

    I. Introduction

    II. Parameters of the Method

    III. Varying Success with ISEM

    IV. Applications

    V. Detection of Double-Stranded RNA

    VI. Preparation of Grids for Later Use

    VII. Problems and Prospects for the Future

    References

    Chapter 4. Quantitative Transmission Electron Microscopy for the Determination of Mass-Molecular Weight of Viruses

    I. Introduction

    II. Theory

    III. Procedure and Treatment of Data to Obtain the Average Dry Mass of Virus Particles

    IV. Applications in Virology

    V. Discussion

    VI. Summary

    References

    Chapter 5. Use of Thin Sectioning for Visualization and Identification of Plant Viruses

    I. Introduction

    II. Technical Procedures for Processing Plant Tissues

    III. Cytological Modifications of Diagnostic Value

    References

    Chapter 6. Purification and Immunological Analyses of Plant Viral Inclusion Bodies

    I. Introduction

    II. Inclusion Purification

    III. SerologicalAnalyses

    IV. Discussion

    References

    Chapter 7. Use of Mosquitoes to Detect and Propagate Viruses

    I. Rationale for Use of the Technique

    II. Technical Considerations

    III. Possible Limitations and Pitfalls

    References

    Chapter 8. Prions: Methods for Assay, Purification, and Characterization

    I. Introduction

    II. Assays for Scrapie Prions

    III. Histopathology

    IV. Purification

    V. Identification and Detection of PrP

    VI. Electron Microscopy

    VII. Polarization Microscopy

    VIII. Biocontainment and Decontamination Procedures

    References

    Chapter 9. Detection of Genome-Linked Proteins of Plant and Animal Viruses

    I. Introduction

    II. Detection of a Genome-Linked Protein (VPg)

    III. Characterization of Genome-Linked Proteins and Adjacent Nucleotide Sequences

    IV. Biosynthesis of Genome-Linked Proteins

    V. Possible Functions of Genome-Linked Proteins

    References

    Index

Product details

  • No. of pages: 414
  • Language: English
  • Copyright: © Academic Press 1984
  • Published: December 7, 1984
  • Imprint: Academic Press
  • eBook ISBN: 9781483259017

About the Editors

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

Hilary Koprowski

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