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Methods in Virology - 1st Edition - ISBN: 9780124702080, 9781483259017

Methods in Virology

1st Edition

Volume VIII

Editors: Karl Maramorosch Hilary Koprowski
eBook ISBN: 9781483259017
Imprint: Academic Press
Published Date: 7th December 1984
Page Count: 414
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Methods in Virology, Volume VIII focuses on the methods used in virology, including microscopy, hybridization, viruses, and fingerprint analysis. The selection first offers information on the hybridization of viral nucleic acids; applications of oligonucleotide fingerprinting to the identification of viruses; and immunosorbent electron microscopy in plant virus studies. Discussions focus on the detection of double-stranded RNA, principles and mechanisms of fingerprint analysis, preparation of labeled nucleic acid probes, and basic methods of nucleic acid hybridization. The text then elaborates on quantitative transmission electron microscopy for the determination of mass-molecular weight of viruses and use of thin sectioning for visualization and identification of plant viruses. Topics include technical procedures for processing plant tissues, cytological modifications of diagnostic value, procedure and treatment of data to obtain the average mass of virus particles, and applications in virology. The book takes a look at the detection of genome-linked proteins of plant and animal viruses; methods for assay, purification, and characterization of prions; and the use of mosquitoes to detect and propagate viruses. The selection is a valuable source of information for researchers interested in the methods employed in virology.

Table of Contents



Contents of Previous Volumes

Chapter 1. Hybridization of Viral Nucleic Acids: Newer Methods on Solid Media and in Solution

I. Introduction

II. Purification of Nucleic Acid

III. Preparation of Labeled Nucleic Acid Probes

IV. Basic Methods of Nucleic Acid Hybridization

V. Southern Blot Hybridization

VI. Northern Blots


Chapter 2. Applications of Oligonucleotide Fingerprinting to the Identification of Viruses

I. Introduction

II. Principles and Mechanisms of Fingerprint Analysis

III. Experimental Procedures

IV. Applications

V. Summary and Conclusions


Chapter 3. Immunosorbent Electron Microscopy in Plant Virus Studies

I. Introduction

II. Parameters of the Method

III. Varying Success with ISEM

IV. Applications

V. Detection of Double-Stranded RNA

VI. Preparation of Grids for Later Use

VII. Problems and Prospects for the Future


Chapter 4. Quantitative Transmission Electron Microscopy for the Determination of Mass-Molecular Weight of Viruses

I. Introduction

II. Theory

III. Procedure and Treatment of Data to Obtain the Average Dry Mass of Virus Particles

IV. Applications in Virology

V. Discussion

VI. Summary


Chapter 5. Use of Thin Sectioning for Visualization and Identification of Plant Viruses

I. Introduction

II. Technical Procedures for Processing Plant Tissues

III. Cytological Modifications of Diagnostic Value


Chapter 6. Purification and Immunological Analyses of Plant Viral Inclusion Bodies

I. Introduction

II. Inclusion Purification

III. SerologicalAnalyses

IV. Discussion


Chapter 7. Use of Mosquitoes to Detect and Propagate Viruses

I. Rationale for Use of the Technique

II. Technical Considerations

III. Possible Limitations and Pitfalls


Chapter 8. Prions: Methods for Assay, Purification, and Characterization

I. Introduction

II. Assays for Scrapie Prions

III. Histopathology

IV. Purification

V. Identification and Detection of PrP

VI. Electron Microscopy

VII. Polarization Microscopy

VIII. Biocontainment and Decontamination Procedures


Chapter 9. Detection of Genome-Linked Proteins of Plant and Animal Viruses

I. Introduction

II. Detection of a Genome-Linked Protein (VPg)

III. Characterization of Genome-Linked Proteins and Adjacent Nucleotide Sequences

IV. Biosynthesis of Genome-Linked Proteins

V. Possible Functions of Genome-Linked Proteins




No. of pages:
© Academic Press 1984
7th December 1984
Academic Press
eBook ISBN:

About the Editors

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

Hilary Koprowski

Ratings and Reviews