Methods in Stem Cell Biology - Part B

Methods in Stem Cell Biology - Part B

1st Edition - August 9, 2022

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  • Editors: Ilio Vitale, Gwenola Manic, Lorenzo Galluzzi
  • Hardcover ISBN: 9780323900188
  • eBook ISBN: 9780323900195

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Description

Methods in Cancer Stem Cell Biology: Part B, Volume 171 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Cell Biology series
  • Includes the latest information on the topic of Methods in Cancer Stem Cell Biology

Readership

Academic, resercahers, government and industrial sectors

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • Contributors
  • Chapter 1: Multiple gene knockdown strategies for investigating the properties of human leukemia stem cells and exploring new therapies
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Notes
  • 5: Concluding remarks
  • Acknowledgments
  • Disclosures
  • References
  • Chapter 2: Evaluation of cancer stem cells markers expression in HCC trough real-time polymerase chain reaction
  • Abstract
  • 1: Introduction and rationale
  • 2: Materials
  • 3: Methods
  • 4: Results and discussion
  • Disclosures
  • References
  • Chapter 3: Reverse Phase Protein Arrays in cancer stem cells
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Notes
  • 5: Concluding remarks
  • Disclosures
  • References
  • Chapter 4: Isolation of cancer stem cells from skin squamous cell carcinoma
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: CSCs assays
  • 5: Precautions to be taken during CSC isolation to avoid commonly faced problems
  • 6: Concluding remarks
  • Disclosures
  • References
  • Chapter 5: Immunoblotting-assisted assessment of JAK/STAT and PI3K/Akt/mTOR signaling in myeloproliferative neoplasms CD34+ stem cells
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Isolation of mononuclear cells (MNCs) from human peripheral blood by density gradient centrifugation
  • 4: Immunomagnetic separation of hematopoietic stem cells (CD34 +) from MNCs
  • 5: Protein extraction and quantification from CD34 + cells
  • 6: Sample preparation and SDS-PAGE Western blotting
  • 7: Membranes probing to detect phosphorylated proteins of JAK/STAT and PI3K/mTOR/Akt pathway
  • 8: Phosphorylated protein revelation and data acquisition
  • 9: Membrane stripping and re-probing to detect total protein of JAK/STAT and PI3K/mTOR/Akt pathway
  • 10: Human colony forming unit (CFU) assay of hematopoietic stem cells (CD34 +)
  • 11: Notes
  • 12: Concluding remarks
  • Disclosures
  • References
  • Chapter 6: Isolation of cancer stem cells from cultured breast cancer cells and xenografted breast tumors based on aldehyde dehydrogenase activity
  • Abstract
  • 1: Background
  • 2: The AldefluorTM assay to isolate putative cancer stem cells
  • 3: The importance of ALDH1A1 and ALDH1A3 in breast cancer
  • 4: Conclusions
  • Acknowledgments
  • References
  • Chapter 7: Intracerebroventricular transplantation of human iPSC-derived neural stem cells (hiPSC-NSCs) into neonatal mice
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Notes
  • 5: Concluding remarks
  • Acknowledgments
  • References
  • Chapter 8: Quantifying epithelial-mesenchymal heterogeneity and EMT scoring in tumor samples via tyramide signal amplification (TSA)
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Imaging and analysis
  • 5: Quantification
  • 6: Concluding Remarks
  • 7: Limitations
  • 8: Notes
  • References
  • Chapter 9: Immunofluorescence staining of colorectal cancer patient-derived organoids
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Method
  • 4: Notes
  • 5: Concluding remarks
  • Disclosures
  • References
  • Chapter 10: Isolation of murine bone marrow hematopoietic stem and progenitor cell populations via flow cytometry
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Notes
  • 5: Concluding remarks
  • Acknowledgments
  • Conflict of interest
  • Disclosures
  • References
  • Chapter 11: Limiting dilution assay to quantify the self-renewal potential of cancer stem cells in hepatocellular carcinoma
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Method
  • 4: NOTES
  • Funding
  • References
  • Chapter 12: Methods to isolate adipose tissue-derived stem cells
  • Abstract
  • 1: Introduction
  • 2: Stromal vascular fraction (SVF) and adipose tissue-derived stem cells (ADSCs)
  • 3: Enzymatic isolation of ADSCs: An overview
  • 4: Non-enzymatic isolation of SVF and ADSCs
  • 5: Novel non-enzymatic systems of ADSCs isolation
  • 6: Conclusions
  • References
  • Chapter 13: Generation of neuronal/glial mixed cultures from human induced pluripotent stem cells (hiPSCs)
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Expected results
  • 5: Notes
  • 6: Concluding remarks
  • Acknowledgments
  • References

Product details

  • No. of pages: 260
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: August 9, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323900188
  • eBook ISBN: 9780323900195

About the Serial Volume Editors

Ilio Vitale

Dr Ilio Vitale is an esteemed oncologist. He is a member of the University of Rome and associated to the Regina Elena National Cancer Institute based in Italy.

Affiliations and Expertise

Regina Elena National Cancer Institute, Rome and Department of Biology, University of Rome, Italy

Gwenola Manic

Gwenola Manic received her Ph.D. from the University of Paris Sud/Paris XI (France) in 2012 studying the impact of the design of HIV-1-derived vectors and DNA repair machinery on lentiviral expression. At present she is working as a postdoctoral fellow at the Department of Biology at the Università degli Studi di Roma “Tor Vergata” in Rome (Italy) on the contribution of chromosomal instability in tumorigenesis and the therapeutic targeting of cancer stem cells.

Affiliations and Expertise

Candiolo Cancer Institute, FPO -IRCCs, Candiolo, Italy

Lorenzo Galluzzi

Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and Expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

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