Methods in Stem Cell Biology - Part B

Methods in Stem Cell Biology - Part B

1st Edition - August 1, 2022

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  • Editors: Ilio Vitale, Gwenola Manic, Lorenzo Galluzzi
  • Hardcover ISBN: 9780323900188

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Methods in Cancer Stem Cell Biology: Part B, Volume 171 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Cell Biology series
  • Includes the latest information on the topic of Methods in Cancer Stem Cell Biology


Academic, resercahers, government and industrial sectors

Table of Contents

  • 1. Multiple Gene Knockdown Strategies for Investigating the Properties of Human Leukemia Stem Cells and Exploring New Therapies
    Xiaoyan Jiang
    2. Assessment of CD133, EpCAM, and CD44 in human and murine cancer cell by real-time qPCR
    Guillermo Mazzolini
    3. Reverse Phase Protein Array in cancer stem cells
    Michele Signore
    4. Isolation of cancer stem cells from squamous cell carcinoma
    Sanjeev K. Centre (TMC), Advanced Centre for Treatment and Research and Education in Cancer (ACTREC),
    5. Immunoblotting-assisted assessment of JAK2 signaling in leukemic stem cells
    Niccolò Bartalucci
    6. Isolation of cancer stem cells from based on aldehyde dehydrogenase activity
    Paola Marcato
    7. Generation of neuronal/glial mixed cultures from human induced pluripotent stem cells (hiPSCs)
    Angela Gritti
    8. Immunohistochemistry-based analysis of EMT markers in mammary tumors
    Diwakar Pattabiraman
    9. Immunofluorescence staining of colorectal cancer patient-derived organoids
    Joseph L. Regan
    10. Isolation of murine bone marrow hematopoietic stem and progenitor cell populations via flow cytometry
    Michael A. Rieger
    11. In vitro and in vivo limiting dilution assay of hepatocellular carcinoma cells
    Bin Wang Sr., Yingying Lai and Xi Zheng
    12. Methods to isolate adipose tissue-derived stem cells
    Barbara Zavan, Paolo Pinton, Leticia Ferroni, Chiara Gardin and Francesco De Francesco
    13. In vivo assessment of the tumorigenic and metastatic potential of the cancer stem cell fraction in breast cancer cells
    Antonella Sistigu

Product details

  • Language: English
  • Copyright: © Academic Press 2022
  • Published: August 1, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323900188

About the Serial Volume Editors

Ilio Vitale

Dr Ilio Vitale is an esteemed oncologist. He is a member of the University of Rome and associated to the Regina Elena National Cancer Institute based in Italy.

Affiliations and Expertise

Regina Elena National Cancer Institute, Rome and Department of Biology, University of Rome, Italy

Gwenola Manic

Gwenola Manic received her Ph.D. from the University of Paris Sud/Paris XI (France) in 2012 studying the impact of the design of HIV-1-derived vectors and DNA repair machinery on lentiviral expression. At present she is working as a postdoctoral fellow at the Department of Biology at the Università degli Studi di Roma “Tor Vergata” in Rome (Italy) on the contribution of chromosomal instability in tumorigenesis and the therapeutic targeting of cancer stem cells.

Affiliations and Expertise

Candiolo Cancer Institute, FPO -IRCCs, Candiolo, Italy

Lorenzo Galluzzi

Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and Expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA

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