Description

Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. This is accompanied by examples of typical results, illustrations, troubleshooting of potential pitfalls, sources of chemicals and equipment, and complete reference lists. Protocols are written to be easily comprehended by beginning research students, but these extensive volumes will also be a valuable addition to the libraries of expert researchers.

Key Features

@introbul:Key Features @bul:* Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants

Readership

Graduate students, technicians, post doctoral, and experienced researchers in cell and developmental biology, plant science, agricultural biotechnology, agronomy, and related segments of plant genetics, molecular biology, biochemistry, and physiology. Especially useful to plant biologists, plant molecular biologists, plant pathologists, plant cell biologists, plant biotechnologists, and biotechnology company personnel.

Table of Contents

Subcellular Fractionation and Analysis of Function: D.W. Galbraith, J. Sheen, R.J. Grebenok, G.M. Lambert, and J. Sheen, Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green Fluorescent Protein. W.F.J. Vermaas, Functional Effects of Structural Changes in Photosystem II as Measured by Chlorophyll Fluorescence Kinetics. J.-K. Zhu, R.A. Bressan, and P.M. Hasegawa, Determination of Protein Isoprenylation in Vitro and in Vivo. W.H. Outlaw, Jr., Extraction and Assay of Protein from Single Plant Cells. J. Feldwich, A. Vente, N. Campos, R. Zettl, and K. Palme, Photoaffinity Labeling and Strategies for Plasma Membrane Protein Purification. S. Lucretti and J. Dolezel, Cell Cycle Synchronization, Chromosome Isolation, and Flow-Sorting in Plants. W.L. Crosby and P. Schorr, Principles and Applications of Recombinant Antibody Phage Display Technology to Plant Biology. T.J. Guilfoyle, Isolation and Characterization of Plant Nuclei. J.C. Cushman, Isolation of Nuclei Suitable for in Vitro Transcriptional Studies. G.E. Schaller and N.D. DeWitt, Analysis of H+-ATPase and Other Proteins of the Arabidopsis Plasma Membrane. J.M. Ward and H. Sze, Isolation and Reconstitution of the Vacuolar H+-ATPase. J.M. Gualberto, H. Handa, and J.M. Grienenberger, Isolation and Fractionation of Plant Mitochondria and Chloroplasts: Specific Examples. H. Wein, B. Brady, and W.Z. Cande, Isolating the Plant Mitotic Appratus: A Procedure for Isolating Spindles from the Diatom Cylindrotheca fusiformis. C.A. Price, N. Hadjeb, L.A. Newman, L.A. Newman, and E.M. Reardon, Chromoplasts. E. Davies and

Details

No. of pages:
555
Language:
English
Copyright:
© 1995
Published:
Imprint:
Academic Press
Print ISBN:
9780125641524
Electronic ISBN:
9780080859484

Reviews

@qu:"...It forms a valuable and readable addition to the literature on plant cell biology..." @source:--P.R. Shewry, IACR--Long Ashton Research Station, in PLANT GROWTH REGULATION @qu:"The text is supplemented with flowcharts of procedures and with instructive figures. This is why the volumes are successfully used by both experienced researchers and students... The traditionally well-edited text is supplemented with a detailed subject index." @source:--Z. Sestak in PHOTOSYNTHETICA @qu:"The present volume competes will in terms of scope and the quality of the individual contribution, many of which contain lengthy introductions as well as detailed methods. In addition, the quality of production is well up to the usual Academic Press standards, including a small number of colour plates. Altogether it forms a valuable and readable addition to the literature on plant cell biology..." @source:--P.R. Shewry in PLANT GROWTH REGULATION