This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international specialists. The biochemical methods include liquid chromatography/electrochemistry, liquid chromatography/fluorescence and chemiluminescence detection, gas chromatography or liquid chromatography with mass spectrometry, and immunoassays.

Morphological and physiological methods are also described, including immunohistochemistry, in situ hybridization, in vivo microdialysis, electrophysiological analysis, microwave irradiation, electrophysiological analysis, micro-anatomical dissection, peptide sequence analysis, quantitative autoradiographic analysis, and isolated CNS preparations. The neurotransmitters and neuropeptides include catecholamines, indoleamines, histamine, polyamines, and various neuropeptides, and their related enzymes. The book is the most comprehensive, advanced and versatile volume on the biochemical, morphological and physiological analysis of neurotransmitters and neuropeptides.

Table of Contents

PART 1. 1. Multi-electrode detection system for determination of neurotransmitters (M. Naoi, W. Maruyama, I.N. Acworth, D. Nakahara, H. Parvez). 2. Determination of biogenic amines, their metabolites, and other neurochemicals by liquid chromatography/electrochemistry (C.T. Duda, P.T. Kissinger). 3. High performance liquid chromatography-fluorometric or chemiluminometric detection of neurotransmitters and their metabolites (K. Imai, H. Watanabe). 4. Combined gas chromatography mass spectrometry for the analysis of biogenic amines (F. Karoum, S. Chrapusta, M. Egan). 5. Immunochemistry and in situ hybridization of catecholamine-synthesizing enzymes and the related neurotransmitters (I. Nagatsu, K. Yamada, M. Sakai, N. Karasawa). 6. Electron microscopic exploration of synaptic organization (D.G. Jones). 7. In vivo microdialysis of neurotransmitters and their metabolites (D. Nakahara, N. Ozaki, T. Nagatsu). 8. Electrophysiological analysis of sympathetic function (H. Togashi, M. Yoshioka, H. Saito). 9. Current status of rapid enzyme inactivation through the use of microwave irradiation (Y. Maruyama, Y. Ikarashi, C.L. Blank, W.B. Stavinoha). 10. Precise micro- and macro-anatomical dissection of the rat brain and the investigation of neuroendocrine function (H. Parvez, S. Parvez, M. Naoi). 11. Analysis of enzymes in catecholamine biosynthesis (K. Kojima, H. Parvez, S. Parvez, T. Nagatsu). 12. Catechol-O-methyltransferase (C.R. Creveling). Subject index. Part 2. 1. Immunoassays of catecholamines and their metabolites (M. Yoshioka). 2. Assay of serotonin, related metabolites and tryptophan hydroxylase (C.J. Schmidt, D.M. Kuhn, W. Lovenberg). 3. Determination of the monoamine oxidases (K.F. Tipton, M.B.H. Youdim). 4. Histamine and its related metabolites (A. Yamatodani, H. Wada). 5. Histamine and its related enzymes (H. Hayashi, T. Watanabe). 6. Histamine receptors (H. Fukui, K. Yanai). 7. Assay methods for polyamines (K. Fujita, T. Nagatsu a


© 1993
Elsevier Science
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