Methods in Neurosciences - 1st Edition - ISBN: 9780121852573, 9781483269368

Methods in Neurosciences

1st Edition

Electrophysiology and Microinjection

eBook ISBN: 9781483269368
Imprint: Academic Press
Published Date: 28th January 1991
Page Count: 520
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Methods in Neurosciences, Volume 4: Electrophysiology and Microinjection is a collection of papers that deals with the electrically excitability feature of many cell types.
This volume describes the characteristic features of some nervous tissue to conduct signals along cellular paths or ""wires."" The text presents such paths as a way stimuli are transferred in the nervous system. Section I reviews the recording methodologies such as those used in measuring noninactivating calcium current in smooth muscles cells or the two-suction electrode voltage-clamp recording. Section II deals in detail with voltage clamping and voltammetry; the text also explains the practical steps in using the current pump-assisted voltage clamp. One paper examines the X-ray microprobe analysis of voltage clamped single heart ventricular myocytes, while another paper explains in vivo voltammetry. Section III addresses electrophysiology and purification of specific receptors; one paper presents the results of an electrophysiological study of hormone secretion by single adenohypophyseal cells. Section IV describes special electrodes and equipment, while Section V deals with special preparations needed in culture preparation or in the study of pharmacology of excitatory amino acids on neurons found in the central nervous system. Chapter VI addresses data analysis and reduction such as digital filtering of bioelectric potentials in personal computers.
This book will prove valuable for microbiologists, cellular scientists, microchemists, and academicians working in the fields of neuroscience.

Table of Contents

Contributors to Volume 4


Volumes in Series

Section I Recording Methodologies

1. Single-Unit Recording in Conscious Sheep

2. Single-Unit Recording from Pontomedullary Neuraxis in Awake, Freely Behaving Animals

3. Measurement of Whole-Cell Calcium Current in Cardiac Myocytes

4. Measurement of Noninactivating Calcium Current in Smooth Muscle Cells

5. Two-Suction Electrode Voltage-Clamp Recording

Section II Voltage Clamping and Voltammetry

6. Voltage-Clamp Measurement of Steady State Currents and Isotope Flux

7. Current Pump-Assisted Voltage-Clamp Apparatus

8. X-Ray Microprobe Analysis of Voltage-Clamped Single Heart Ventricular Myocytes

9. In Vivo Voltammetry

10. Simultaneous In Vivo Voltammetric and Electrophysiological Recording with Carbon Fiber Microelectrodes

Section III Electrophysiology and Purification of Specific Receptors

11. Electrophysiological Expression of Ion Channels in Xenopus Oocytes

12. Electrophysiology of Neuronal Nicotinic Acetylcholine Receptors Expressed in Xenopus Oocytes following Nuclear Injection of Genes or cDNAs

13. Electrophysiological Study of Hormone Secretion by Single Adenohypophyseal Cells

14. Purification of L-Type Calcium Channel Drug Receptors

Section IV Special Electrodes and Equipment

15. Movable Intracranial Stimulating Electrode System, Electroencephalogram, and Evoked Potential Recording in Pigs and Sheep

16. Multiple Microelectrodes

17. Oil and Hook Electrodes for en Passant Recording from Small Nerves

18. Measurement of Calcium Flux and Intracellular Sodium by Ion-Selective Microelectrodes

19. Microincubator for Regulating Temperature and Superfusion of Tissue-Cultured Neurons during Electrophysiological or Optical Studies

Section V Special Preparations

20. Multicompartment Cell Cultures for Studies of Neuronal Interactions

21. Grease-Gap Methods for Studying Pharmacology of Excitatory Amino Acids on Central Nervous System Neurons

22. Perforated Patch Recording

23. Patch-Clamp Measurements of Ion Channels in Biomembrane Vesicles Reconstituted into Giant Proteoliposomes by Freeze-Thawing without Use of Detergent

24. Injections into Mouse Sciatic Nerve for In Vivo Studies of Quantitative, Short-Term Metabolism

Section VI Data Analysis and Reduction

25. Digital Filtering of Potentials on Personal Computers

26. Fitting of Single-Channel Dwell Time Distributions

27. Discrimination of Kinetic Models of Ion Channel Gating

28. Voltage Noise Analysis in Small Cells

29. List-Oriented Analysis of Single-Channel Data



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© Academic Press 1991
Academic Press
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