Methods in Mycoplasmology V2 - 1st Edition - ISBN: 9780125838023, 9780323149396

Methods in Mycoplasmology V2

1st Edition

Diagnostic Mycoplasmology

Editors: Joseph Tully
eBook ISBN: 9780323149396
Imprint: Academic Press
Published Date: 28th January 1983
Page Count: 464
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Description

Methods in Mycoplasmology, Volume II: Diagnostic Mycoplasmology is the second of a two-volume series that resulted from cumulative efforts to meet the need for standardized techniques in mycoplasmology. The book is organized into five sections. Section A discusses techniques for isolating mycoplasmas from humans. These include the recovery of mycoplasmas from the respiratory and genitourinary tracts, from blood and special tissues, and serological Identification of mycoplasmas from humans. Section B focuses on techniques for isolating mycoplasmas from animals, including the recovery and identification of avian, bovine, caprine, ovine, canine, and feline mycoplasmas. Section C presents techniques for isolating mycoplasmas from cell cultures. These include agar and broth techniques, the use of indicator cell lines, and DNA fluorochrome staining. Section D discusses techniques for isolating mycoplasmas from plants and insects. Section E describes some of the techniques that are useful in evaluating the pathogenicity of mycoplasmas, ureaplasmas, and spiroplasmas. Section F defines some of the techniques useful in virus detection by electron microscopy and presents methods for isolating and characterizing viruses from both mycoplasmas and spiroplasmas.

Table of Contents


Contributors

Preface

Contents of Volume I

Section A Special Techniques for Isolation and Identification of Mycoplasmas from Humans

A1 Introductory Remarks

A2 Recovery of Mycoplasmas from the Respiratory Tract

A3 Recovery of Mycoplasmas from the Genitourinary Tract

A4 Recovery of Mycoplasmas from Blood and Special Tissues

A5 Serological Identification of Mycoplasmas from Humans

A6 The Complement Fixation Test for Diagnosis of Mycoplasma pneumoniae Infection

A7 Serological Identification of Ureaplasmas from Humans

Section Β Special Techniques for Isolation and Identification of Mycoplasmas from Animals

B1 Introductory Remarks

B2 Recovery and Identification of Avian Mycoplasmas

B3 Recovery and Identification of Bovine Mycoplasmas

B4 Recovery and Identification of Caprine and Ovine Mycoplasmas

B5 Recovery and Identification of Canine and Feline Mycoplasmas

B6 Recovery of. Identification of, and Serological Response to Porcine Mycoplasmas

B7 Recovery and Identification of Murine Mycoplasmas

B8 Recovery and Identification of Mycoplasmas from Other Laboratory Animals (Including Primates)

B9 Lavage Techniques for Recovery of Animal Mycoplasmas

Section C Special Techniques for Isolation and Identification of Mycoplasmas from Cell Cultures

C1 Introductory Remarks

C2 Isolation of Mycoplasmas from Cell Cultures by Agar and Broth Techniques

C3 Use of Indicator Cell Lines for Recovery and Identification of Cell Culture Mycoplasmas

C4 Detection and Identification of Mycoplasmas in Infected Cell Cultures by Direct Immunofluorescence Staining

C5 Detection of Mycoplasmal Infection of Cell Cultures by DNA Fluorochrome Staining

C6 Biochemical Procedures for the Detection of Mycoplasmal Infection in Cell Cultures

C7 Prevention and Control of Mycoplasmal Infection of Cell Cultures

Section D Special Techniques for Isolation and Identification of Mycoplasmas from Plants and Insects

D1 Introductory Remarks

D2 Culture Techniques for Spiroplasmas from Arthropods

D3 Culture Techniques for Spiroplasmas from Plants

D4 Culture Techniques for Plant Mycoplasmas

D5 Physical Techniques for Purification of Mycoplasmas from Plant Tissues

D6 Special Serological Tests for Spiroplasma Identification

D7 Transmission Techniques with Vectors of Plant and Insect Mycoplasmas and Spiroplasmas

D8 Antibiotic Treatment of Plants and Bioassay to Determine Foliar Antibiotic Concentration

D9 Treatment of Woody Plants with Antibiotics

Section Ε Pathogenicity Tests and Antibiotic Sensitivity Testing

E1 Introductory Remarks

E2 Respiratory Challenge of Experimental Animals

E3 Intramammary Inoculation of Cattle and Other Animals

E4 Evaluation of Arthritogenic Properties of Mycoplasmas for Small Laboratory Animals

E5 Tests for Mycoplasma neurolyticum Neurotoxin

E6 Pathogenicity Tests in Cell Cultures

E7 Genitourinary Challenge in Various Hosts

E8 Tracheal Organ Cultures as Models in Pathogenicity Studies

E9 Fallopian Tube Cultures as Models in Pathogenicity Studies

E10 Tests for Adherence Properties of Mycoplasmas

E11 Demonstration of Phytotoxin Production by Spiroplasmas

E12 Pathogenicity of Mycoplasmas for Arthropods and Its Possible Significance in Biological Control

E13 Special Procedures for Demonstration of Mycoplasma Pathogenicity in Insects

E14 Determination of Spiroplasma Pathogenicity by Inoculation of Suckling Rats and Embryonated Chicken Eggs

E15 Assays for Lymphocyte Activation

E16 Antibiotic Sensitivity Testing of Mycoplasmas

Section F Mycoplasma Viruses

F1 Introductory Remarks

F2 Virus Detection by Electron Microscopy

F3 Isolation and Characterization of Mycoplasma Viruses

F4 MV-L2: Growth and Purification

F5 Isolation and Characterization of Spiroplasma Viruses

Index


Details

No. of pages:
464
Language:
English
Copyright:
© Academic Press 1983
Published:
Imprint:
Academic Press
eBook ISBN:
9780323149396

About the Editor

Joseph Tully

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