Methods in Methane Metabolism, Part A

Methods in Methane Metabolism, Part A

Methanogenesis

1st Edition - March 24, 2011
This is the Latest Edition
  • Editors: Amy Rosenzweig, Stephen Ragsdale
  • eBook ISBN: 9780123851130
  • Hardcover ISBN: 9780123851123

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Description

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

Key Features

  • Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements
  • The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field

Readership

Researchers and the wider scientific community

Table of Contents

  • 1. Techniques for Cultivating Methanogens
    Ralph S. Wolfe
    2. Genetic Methods for Methanosarcina species
    Nicole Buan, Gargi Kulkarni and William Metcalf
    3. Genetic Systems for Hydrogenotrophic Methanogens
    Felipe Sarmiento B, John A. Leigh and William B. Whitman
    4. Molecular tools for investigating ANME community structure and function
    Steven J. Hallam, Antoine Pagé, Lea Constan, Young C. Song, Angela Norbeck, Heather Mottaz, and Ljiljana Pasa-Tolic
    5. Studying gene regulation in methanogenic archaea
    Michael Rother, Christian Sattler and Tilmann Stock
    6. Growth of Methanogens Under Defined Hydrogen Conditions
    John A. Leigh
    7. Preparation of [Fe]-hydrogenase from methanogenic archaea
    Seigo Shima, Michael Schick and Haruka Tamur
    8. Assay of methylotrophic methyltransferases from methanogenic archaea
    Donald J. Ferguson, David G. Longstaff and Joseph A. Krzycki
    9. Methyl-Coenzyme M Reductase from Methanothermobacter marburgensis
    Evert C. Duin, Divya Prakash, Charlene Brungess
    10. Methods for Analysis of Acetyl-CoA Synthase: Applications to bacterial and archaeal systems
    David A. Grahame
    11. Acetate Kinase and Phosphotransacetylase
    James G. Ferry
    12. Sodium ion translocation and ATP synthesis in methanogens
    Katharina Schlegel and Volker Müller
    13. Proton translocation in methanogens
    Cornelia Welte and Uwe Deppenmeier
    14. Measuring isotope fractionation by autotrophic microorganisms and enzymes
    Kathleen M. Scott, Gordon Fox and Peter R. Girguis
    15. 2-Oxoacid Metabolism in Methanogenic CoM and CoB Biosynthesis
    David E. Graham
    16. Biomethanation and its potential
    Irini Angelidaki, Fons Stams and Dimitar Karakashev

Product details

  • No. of pages: 424
  • Language: English
  • Copyright: © Academic Press 2011
  • Published: March 24, 2011
  • Imprint: Academic Press
  • eBook ISBN: 9780123851130
  • Hardcover ISBN: 9780123851123
  • About the Editors

    Amy Rosenzweig

    Stephen Ragsdale