Methods in Methane Metabolism, Part A - 1st Edition - ISBN: 9780123851123, 9780123851130

Methods in Methane Metabolism, Part A, Volume 494

1st Edition

Methanogenesis

Editors: Amy Rosenzweig Stephen Ragsdale
eBook ISBN: 9780123851130
Hardcover ISBN: 9780123851123
Imprint: Academic Press
Published Date: 24th March 2011
Page Count: 424
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Table of Contents

1. Techniques for Cultivating Methanogens
Ralph S. Wolfe
2. Genetic Methods for Methanosarcina species
Nicole Buan, Gargi Kulkarni and William Metcalf
3. Genetic Systems for Hydrogenotrophic Methanogens
Felipe Sarmiento B, John A. Leigh and William B. Whitman
4. Molecular tools for investigating ANME community structure and function
Steven J. Hallam, Antoine Pagé, Lea Constan, Young C. Song, Angela Norbeck, Heather Mottaz, and Ljiljana Pasa-Tolic
5. Studying gene regulation in methanogenic archaea
Michael Rother, Christian Sattler and Tilmann Stock
6. Growth of Methanogens Under Defined Hydrogen Conditions
John A. Leigh
7. Preparation of [Fe]-hydrogenase from methanogenic archaea
Seigo Shima, Michael Schick and Haruka Tamur
8. Assay of methylotrophic methyltransferases from methanogenic archaea
Donald J. Ferguson, David G. Longstaff and Joseph A. Krzycki
9. Methyl-Coenzyme M Reductase from Methanothermobacter marburgensis
Evert C. Duin, Divya Prakash, Charlene Brungess
10. Methods for Analysis of Acetyl-CoA Synthase: Applications to bacterial and archaeal systems
David A. Grahame
11. Acetate Kinase and Phosphotransacetylase
James G. Ferry
12. Sodium ion translocation and ATP synthesis in methanogens
Katharina Schlegel and Volker Müller
13. Proton translocation in methanogens
Cornelia Welte and Uwe Deppenmeier
14. Measuring isotope fractionation by autotrophic microorganisms and enzymes
Kathleen M. Scott, Gordon Fox and Peter R. Girguis
15. 2-Oxoacid Metabolism in Methanogenic CoM and CoB Biosynthesis
David E. Graham
16. Biomethanation and its potential
Irini Angelidaki, Fons Stams and Dimitar Karakashev


Description

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

Key Features

  • Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements
  • The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field

Readership

Researchers and the wider scientific community


Details

No. of pages:
424
Language:
English
Copyright:
© Academic Press 2011
Published:
Imprint:
Academic Press
eBook ISBN:
9780123851130
Hardcover ISBN:
9780123851123

About the Editors

Amy Rosenzweig Editor

Stephen Ragsdale Editor