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1. Generation of primary cells from ADPKD and normal human kidneys
Darren P. Wallace and Gail A. Reif
2. Measurement of cytoplasmic and cilioplasmic calcium in a single living cell
Rinzhin T. Sherpa, Rajasekharreddy Pala, Ashraf M. Mohieldin and Surya M. Nauli
3. Application of physiological shear stress to renal tubular epithelial cells
Nicholas Ferrell, Ruben M. Sandoval, Bruce A. Molitoris, Paul Brakeman, Shuvo Roy and William H. Fissell
4. ADPKD cell proliferation and Cl−-dependent fluid secretion
Gail A. Reif and Darren P. Wallace
5. In vitro cyst formation of ADPKD cells
Madhulika Sharma, Gail A. Reif and Darren P. Wallace
6. Engineering kidney tissues for polycystic kidney disease modeling and drug discovery
Valerio Brizi, Valentina Benedetti, Angelo Michele Lavecchia and Christodoulos Xinaris
7. Differentiation of human kidney organoids from pluripotent stem cells
Nelly M. Cruz and Benjamin S. Freedman
8. Studying Na+ and K+ channels in aldosterone-sensitive distal nephrons
Jacques Teulon and Wen-Hui Wang
9. Metanephric organ culture
Robin L. Maser, Brenda S. Magenheimer and James P. Calvet
10. Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells
Biagio Saitta, Michael F. Jalili, Hamidreza Zohoorkari, Renee Rao, Kenneth R. Hallows, Catherine J. Baty and Nuria M. Pastor-Soler
11. Analysis of primary cilia in renal tissue and cells
Luciane M. Silva, Wei Wang, Bailey A. Allard, Tana S. Pottorf, Damon T. Jacobs and Pamela V. Tran
12. Quantifying autophagic flux in kidney tissue using structured illumination microscopy
Kensei Taguchi, Bertha C. Elias, Subo Qian and Craig R. Brooks
13. Investigation of epigenetics in kidney cell biology
Linda Xiaoyan Li, Ewud Agborbesong, Lu Zhang and Xiaogang Li
Methods in Kidney Cell Biology, Volume 153, represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, and clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
- Written by experts in their field who have perfected the methods they write about
- Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form
- Includes cutting-edge, recently developed methods
Anyone conducting renal research from novice to veteran and from technicians to graduate students to postdoctoral fellows and principal investigators
- No. of pages:
- © Academic Press 2019
- 3rd August 2019
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
Dr. Weimbs received his doctoral degree from the Department of Biochemistry of the University of Cologne, Germany, in 1993. He conducted postdoctoral research with Keith Mostov at the Department of Anatomy, University of California at San Francisco until 1999 where he investigated the role of SNARE proteins in membrane trafficking and epithelial cell polarity. In 1999, he joined the Department of Cell Biology in the Lerner Research Institute of the Cleveland Clinic as an Assistant Professor where he established his own research laboratory. Besides continuing his work on SNAREs and epithelial cell polarity his laboratory began to investigate molecular mechanisms underlying polycystic kidney disease (PKD). In 2005, Dr. Weimbs moved his lab to the University of California in Santa Barbara where he is currently a Professor in the Department of Molecular, Cellular, and Developmental Biology. Research on PKD in Dr. Weimbs’ laboratory has contributed to our understanding of the molecular pathogenesis and the function of polycystin-1, the protein affected in this disease. These contributions include the roles of mTOR and STAT signaling in PKD. Recent work has focused on developing new kidney-targeted therapeutics, the role of metabolic changes and tubular crystal deposition in PKD disease progression, and the use of dietary interventions for PKD therapy.
University of California, Santa Barbara, USA
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