
Methods in Kidney Cell Biology Part A
Description
Key Features
- Written by experts in their field who have perfected the methods they write about
- Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form
- Includes cutting-edge, recently developed methods
Readership
Anyone conducting renal research from novice to veteran and from technicians to graduate students to postdoctoral fellows and principal investigators
Table of Contents
1. Generation of primary cells from ADPKD and normal human kidneys
Darren P. Wallace and Gail A. Reif
2. Measurement of cytoplasmic and cilioplasmic calcium in a single living cell
Rinzhin T. Sherpa, Rajasekharreddy Pala, Ashraf M. Mohieldin and Surya M. Nauli
3. Application of physiological shear stress to renal tubular epithelial cells
Nicholas Ferrell, Ruben M. Sandoval, Bruce A. Molitoris, Paul Brakeman, Shuvo Roy and William H. Fissell
4. ADPKD cell proliferation and Cl−-dependent fluid secretion
Gail A. Reif and Darren P. Wallace
5. In vitro cyst formation of ADPKD cells
Madhulika Sharma, Gail A. Reif and Darren P. Wallace
6. Engineering kidney tissues for polycystic kidney disease modeling and drug discovery
Valerio Brizi, Valentina Benedetti, Angelo Michele Lavecchia and Christodoulos Xinaris
7. Differentiation of human kidney organoids from pluripotent stem cells
Nelly M. Cruz and Benjamin S. Freedman
8. Studying Na+ and K+ channels in aldosterone-sensitive distal nephrons
Jacques Teulon and Wen-Hui Wang
9. Metanephric organ culture
Robin L. Maser, Brenda S. Magenheimer and James P. Calvet
10. Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells
Biagio Saitta, Michael F. Jalili, Hamidreza Zohoorkari, Renee Rao, Kenneth R. Hallows, Catherine J. Baty and Nuria M. Pastor-Soler
11. Analysis of primary cilia in renal tissue and cells
Luciane M. Silva, Wei Wang, Bailey A. Allard, Tana S. Pottorf, Damon T. Jacobs and Pamela V. Tran
12. Quantifying autophagic flux in kidney tissue using structured illumination microscopy
Kensei Taguchi, Bertha C. Elias, Subo Qian and Craig R. Brooks
13. Investigation of epigenetics in kidney cell biology
Linda Xiaoyan Li, Ewud Agborbesong, Lu Zhang and Xiaogang Li
Product details
- No. of pages: 292
- Language: English
- Copyright: © Academic Press 2019
- Published: August 3, 2019
- Imprint: Academic Press
- eBook ISBN: 9780128170830
- Hardcover ISBN: 9780128170823
About the Serial Volume Editor
Thomas Weimbs

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