Methods in Gene Technology, Volume 2 - 1st Edition - ISBN: 9781559382649

Methods in Gene Technology, Volume 2

1st Edition

Editors: J.W. Dale P.G. Sanders
Hardcover ISBN: 9781559382649
Imprint: Elsevier Science
Published Date: 1st March 1995

Institutional Access


Table of Contents

Contents. List of Contributors. Introduction. Purification of DNA (J.W. Dale). Electrophoretic Separation of Nucleic Acids (P.G. Sanders0. Preparative Gel Electrophoresis (J.C. Maule). Plasmid Isolation Procedures (A.I. Knight). Plasmid Isolation: column Methods (T.J. Hellyer0. Isolation of Large Plasmids (G.L. Jones and P.A. Williams). Isolation of Linear and Single-Strand Plasmid DNA (J.C. Alonso). Preparation of Bacteriophage &lgr; DNA (E. Norman). Purification of Viral RNA (J.E. Whitby, A.D.T. Barrett, A.D. Jennings, and P. Johnstone). Large Scale DNA Sequencing by Manual Methods (A.J. Davison and E.A.R. Telford). Pulsed-Field Gel Electrophoresis: Applications to Bacteria (P.R. Stewart, W. El-Adhami, and B. Inglis). Pulsed-Field Gel Electrophoresis: Large Scale Restriction Maps (N. Okahashi and T. Koga). Pulsed-Field Gel Electrophoresis: Isolation and Analysis of Large Linear Plasmids (H. Kinashi). Detection of Mutations by PCR-Single-Strand Conformation Polymorphism (A. Telenti). Analysis of DNA Supercoiling (K. Drlica, M. Malik, J.Y. Wang, A. Sasiak and R.R. Sinden). Gel Retardation Assays and DNA Footprinting (I.S. Goping, D.A. Underhill, and G.C. Shore). Southwestern Blot (G. Corte and M.T. Corsetti). Genomic Fingerprinting Using the PCR-Random Amplified Polymorphic DNA Technique (S.V.P.S. Silva and J.D. Procunier). PCR Walking (M.G. Burdon and J.J. Willoughby). Index.

Details

Language:
English
Copyright:
© Elsevier Science 1994
Published:
Imprint:
Elsevier Science
Hardcover ISBN:
9781559382649

About the Editor

J.W. Dale

Affiliations and Expertise

Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, England

P.G. Sanders

Affiliations and Expertise

Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, England