Methods for Plant Molecular Biology

Table of Contents

List of Contributors

Preface

Contents of Methods in Enzymology,Volume 118

Section I. Cell Wall and Membrane


1. Isolation and Characterization of Plant Cell Walls and Cell Wall Components


2. Isolation of the Plasma Membrane

Section II. Nucleus


3. Purification and Restriction Endonuclease Analysis of Plant Nuclear DNA


4. Analyzing Genome Variation in Plants

Section III. Cytoplasmic Protein Synthesis


5. The Synthesis of High-Molecular-Weight Proteins in the Wheat Germ Translation System

Section IV. The Chloroplast

A. Genome Structure


6. Isolation and Structural Analysis of Chloroplast

B. Transcription-Translation


7. In Vitro Transcription of Chloroplast Protein Genes


8. Coupled Transcription-Translation in Chloroplast


9. Translation by Isolated Pea Chloroplasts

C. Photosynthetic Systems


10. Isolation and Characterization of Chloroplast Envelope Membranes


11. Characterization of the Thylakoid Membrane by Subfractionation Analyses


12. Subunit Structure and Biogenesis of ATP Synthase and Photosystem I Reaction Center

D. RuBP Carboxylase


13. Cloning and Expression of Genes for the Small Subunit of Ribulosebisphosphate Carboxylase


14. Separation and Reassembly of the Subunits of Ribulosebisphosphate Carboxylase


15. The Cloning and Expression in Escherichia coli of RuBP Carboxylase/Oxygenase Large Subunit Genes

Section V. Mitochondria


16. The Isolation of Mitochondria and Mitochondrial


17. Analysis of the Genome Structure of Plant Mitochondria


18. Strategies for the Identification and Analysis of Higher Plant Mitochondrial Genes


19. Isolation of Plant Mitochondrial

Section VI. Nitrogen Metabolism


20. Isolation and Characterization of Nitrogenase from Klebsiella pneumonia

Section VII. Cell Culture and Transformation


22. Establishment of Calli and Suspension Cultures


23. Protoplasts: Isolation, Culture, Plant Regeneration


24. Fusion and Transformation of Plant Protoplasts


25. Organelle Transfer

Section VIII. Gene Transfer


26. Gene Transfer in Plants: Production of Transformed Plants Using Ti Plasmid Vectors


27. Plant Virus Vectors: Cauliflower Mosaic Virus


28. Direct Gene Transfer to Plants

Section IX. Virology


29. In Vitro Transcription of Infectious Viral RNA from Cloned cDNA


30. Use of RNA Probes to Detect Plant RNA Viruses


31. Preparation and Use of cDNA Probes for Detection of Viral Genomes


32. ELISA Techniques

Subject Index