Description

This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

Key Features

  • Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
  • Chapters are written by experts in the field
  • Cutting-edge material

Readership

Researchers and students in cell, molecular and developmental biology

Table of Contents

Series Page

Contributors

Preface

Part 1: Model Systems and Functional Studies

Chapter 1. Analysis of Golgi Complex Functions: In Vitro Reconstitution Systems

Abstract

Introduction

Acknowledgments

References

Chapter 2. RNA Interference Approaches to Examine Golgi Function in Animal Cell Culture

Abstract

Overview of Gene Depletion Methodologies

2.1 Key Considerations for the Analysis of Organelle Function

2.2 Strategies for RNAi Experiments

2.3 Choosing Between siRNA and shRNA

2.4 Sources of Reagents for RNAi

2.5 Controls

2.6 Methods

2.7 Discussion

Summary

Acknowledgments

References

Chapter 3. Trafficking Along the Secretory Pathway in Drosophila Cell Line and Tissues: A Light and Electron Microscopy Approach

Abstract

Introduction and Rationale

3.1 Materials

3.2 Methods

Conclusions/Perspectives

Acknowledgments

References

Chapter 4. Studying Membrane Trafficking in the Worm C. elegans by RNA Interference

Abstract

Introduction and Rationale

4.1 Materials

4.2 Methods

4.3 Discussion

Summary

Acknowledgment

References

Chapter 5. Endomembrane and Golgi Traffic in Plant Cells

Abstract

Introduction

5.1 Leaves as a Model System for Analyzing the Plant Golgi and Secretory Pathway

5.2 Mutant Screens for Trafficking Proteins

5.3 Photobleaching for Transport Studies

5.4 Förster Resonance Energy Transfer for Protein Interactions

Summary

Acknowledgments

References

Chapter 6. Biochemical Analysis of Secretory Trafficking in Mammalian Cells

Abstract

Introduction

6.1 Materials

6.2 Methods

6.3 Discussion

Summary

Acknowledg

Details

No. of pages:
498
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Electronic ISBN:
9780124171831
Print ISBN:
9780124171640

About the serial-volume-editors

David Stephens

School of Biochemistry, Bristol University, UK