Description

This book provides up-to-date, state-of-the-art methods for the purification of metallothionein and related proteins, for the study of metal binding, for the quantitation of metallothionein, and for the study of the physical and biological properties of metallothionein. New views of the structure and function of metallothionein are also included in some chapters.

Readership

Biochemists, cell biologists, molecular biologists, biophysicists, analytical chemists, physical chemists, and immunologists.

Table of Contents

Introduction: B.L. Vallee, Introduction to Metallothionein. Y. Kojima, Definitions and Nomenclature of Metallothioneins. D.M. Templeton and M.G. Cherian, Toxicological Significance of Metallothionein. I. Bremner, Nutritional and Physiologic Significance of Metallothionein. Isolation of Metallothioneins: M. Va~js~aaak, Large-Scale Preparation of~Metallothionein: Biological Sources. M. Va~js~aaak, Standard Isolation Procedure for Metallothionein. M. Va~js~aaak, Criteria of Purity for Metallothioneins. K.H. Falchuk and M. Czupryn, Isolation of Metallothioneins under Metal-Free Conditions. Quantification in Tissues and Body Fluids: K.H. Summer and D. Klein, Determination of Metallothionein in Biological Materials. I. Bremner and R.K. Mehra, Assay of Extracellular Metallothionein. C.C. McCormick and L.-Y. Lin, Quantification and Identification of Metallothioneins by Gel Electrophoresis and Silver Staining. A.M. Scheuhammer and M.G. Cherian, ~Quantification of Metallothionein by Silver Saturation. D.L. Eaton and M.G. Cherian, Determination of Metallothionein in Tissues by Cadmium-Hemoglobin Affinity Assay. M.G. Cherian and D. Banerjee, Immunohistochemical Localization of Metallothionein. B. Jasani and M.E. Elmes, Immunohistochemical Detection of Metallothionein. Y. Aoki and K.T. Suzuki, Detection of Metallothionein by Western Blotting. M. Kimura, S. Koizumi, and F. Otsuka, Detection of Carboxymethylmetallothionein by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Z.A. Shaikh, Radioimmunoassay for

Details

No. of pages:
681
Language:
English
Copyright:
© 1991
Published:
Imprint:
Academic Press
Print ISBN:
9780121821067
Electronic ISBN:
9780080883199

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY