Metallobiochemistry, Part B: Metallothionein and Related Molecules, Volume 205

1st Edition

Serial Volume Editors: James Riordan Bert Vallee
Print ISBN: 9780121821067
eBook ISBN: 9780080883199
Imprint: Academic Press
Published Date: 26th November 1991
Page Count: 681

Institutional Access


This book provides up-to-date, state-of-the-art methods for the purification of metallothionein and related proteins, for the study of metal binding, for the quantitation of metallothionein, and for the study of the physical and biological properties of metallothionein. New views of the structure and function of metallothionein are also included in some chapters.


Biochemists, cell biologists, molecular biologists, biophysicists, analytical chemists, physical chemists, and immunologists.

Table of Contents


B.L. Vallee, Introduction to Metallothionein.

Y. Kojima, Definitions and Nomenclature of Metallothioneins.

D.M. Templeton and M.G. Cherian, Toxicological Significance of Metallothionein.

I. Bremner, Nutritional and Physiologic Significance of Metallothionein.

Isolation of Metallothioneins:

M. Va~js~aaak, Large-Scale Preparation of~Metallothionein: Biological Sources.

M. Va~js~aaak, Standard Isolation Procedure for Metallothionein.

M. Va~js~aaak, Criteria of Purity for Metallothioneins.

K.H. Falchuk and M. Czupryn, Isolation of Metallothioneins under Metal-Free Conditions.

Quantification in Tissues and Body Fluids:

K.H. Summer and D. Klein, Determination of Metallothionein in Biological Materials.

I. Bremner and R.K. Mehra, Assay of Extracellular Metallothionein.

C.C. McCormick and L.-Y. Lin, Quantification and Identification of Metallothioneins by Gel Electrophoresis and Silver Staining.

A.M. Scheuhammer and M.G. Cherian, ~Quantification of Metallothionein by Silver Saturation.

D.L. Eaton and M.G. Cherian, Determination of Metallothionein in Tissues by Cadmium-Hemoglobin Affinity Assay.

M.G. Cherian and D. Banerjee, Immunohistochemical Localization of Metallothionein.

B. Jasani and M.E. Elmes, Immunohistochemical Detection of Metallothionein.

Y. Aoki and K.T. Suzuki, Detection of Metallothionein by Western Blotting.

M. Kimura, S. Koizumi, and F. Otsuka, Detection of Carboxymethylmetallothionein by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis.

Z.A. Shaikh, Radioimmunoassay for


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© Academic Press 1991
Academic Press
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About the Serial Volume Editor

James Riordan

Affiliations and Expertise

Harvard Medical School, Boston, Massachusetts, U.S.A.

Bert Vallee

Affiliations and Expertise

Harvard Medical School, Boston, Massachusetts, U.S.A.


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