Metabolons and Supramolecular Enzyme Assemblies

Metabolons and Supramolecular Enzyme Assemblies

1st Edition - February 19, 2019

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  • Editors: Claudia Schmidt-Dannert, Maureen Quin
  • Hardcover ISBN: 9780128170748
  • eBook ISBN: 9780128170755

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Metabolons and Supramolecular Enzyme Assemblies, Volume 617 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Dynamic plant metabolons, TCA cycle metabolons, the Chemotactic assembly of metabolons, Repurposing peroxisomes for metabolic engineering, Repurposing yeast mitochondria for metabolic engineering, Repurposing plant compartments for metabolic engineering, Protein scaffolds for pathway co-localization on lipid droplets, Engineered enzyme assemblies for metabolic engineering, NRPS assembly lines and P450 interactions, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in this series on enzymology
  • Updated release includes the latest information on dynamic plant metabolons, TCA cycle metabolons, the chemotactic assembly of metabolons, and much more


Biochemists, chemists, microbiologists, microbiome researchers, marine scientists, protein chemists, biotechnologists, chemical biologists, blue biotech specialists, natural product chemists, drug discovery experts

Table of Contents

  • 1. Molecular snapshots of dynamic membrane-bound metabolons
    Jean-Etienne Bassard and Tomas Laursen
    2. Tricarboxylic acid metabolon
    Fei Wu and Shelley Minteer
    3. Metabolon formation by chemotaxis
    Xi Zhao and Ayusman Sen
    4. Cloning, expression, and purification of intact polyketide synthase modules
    Laurence Maschio, Alice E. Parnell, Nicholas R. Lees, Christine L. Willis, Christiane Schaffitzel, James E.M. Stach and Paul R. Race
    5. Repurposing peroxisomes for microbial synthesis for biomolecules
    Jiaoqi Gao and Yongjin J. Zhou
    6. Studying trans-acting enzymes that target carrier protein bound amino acids during nonribosomal peptide synthesis
    Anja Greule, Louise K. Charkoudian and Max J. Cryle
    7. Cargo encapsulation in bacterial microcompartments: Methods and analysis
    Taylor Nichols, Nolan Kennedy and Danielle Tullman-Ercek
    8. Bacterial bioreactors: Outer membrane vesicles for enzyme encapsulation
    Kendrick B. Turner, Scott N. Dean and Scott A. Walper
    9. TXTL-based approach to synthetic cells
    Jonathan Garamella, David Garenne and Vincent Noireaux
    10. Cell-surface display of designer cellulosomes by Lactobacillus plantarum
    Yonit Ben-David, Sarah Morais, Johanna Stern, Itzhak Mizrahi and Edward A. Bayer
    11. Enzyme colocalization in protein-based hydrogels
    Louis Lancaster, Beyza Bulutoglu, Scott Banta and Ian Wheeldon
    12. DNA binding adaptors to assemble proteins of interest on DNA scaffold
    Eiji Nakata, Huyen Dinh, Thang Minh Nguyen and Takashi Morii
    13. Protein-based scaffolds for enzyme immobilization
    Guoqiang Zhang, Sarah Schmidt-Dannert, Maureen Quin and Claudia Schmidt-Dannert
    14. Artificial scaffolds for enhanced biocatalysis
    Qing Sun, Shen-Long Tsai, and Wilfred Chen
    15. On-pot and cell-free biocatalysis using coimmobilized enzymes on advanced materials
    Fernando López-Gallego
    16. Combinatorial biosynthesis of small molecules in plants: Engineering strategies and tools
    Iryna Gerasymenko, Yuriy Sheludko, Sabine Fräbel, Agata Staniek and Heribert Warzecha
    17. Insider information on successful covalent protein coupling with help from SpyBank
    Anthony H. Keeble and Mark Howarth
    18. EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors
    Sally Jones, Andrea Martella and Yizhi Cai

Product details

  • No. of pages: 511
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: February 19, 2019
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128170748
  • eBook ISBN: 9780128170755

About the Serial Volume Editors

Claudia Schmidt-Dannert

Claudia Schmidt-Dannert is at the University of Minnesota, USA

Affiliations and Expertise

University of Minnesota, USA

Maureen Quin

Maureen Quin works at the University of Minnesota, USA

Affiliations and Expertise

University of Minnesota, USA

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