I. Strategies and Techniques
Purification Strategies for Membrane Proteins.
Chromatographic Techniques and Basic Operations in Membrane Protein Purification.
Production and purification of recombinant membrane proteins.
SDS Electrophoresis Techniques.
Preparative Isoelectric Focussing.
Membrane protein crystallization.
II. Discussion of Selected Isolation Protocols
Lipid Dependent Inactivation and Reactivation of Bovine complex III.
Purification of affinity-epitope tagged G-protein coupled receptors.
Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D
Purification of the yeast cytochrome bc1 complex.
III. Crystallization of Membrane Proteins
Antibody fragment-mediated crystallization of membrane proteins.
Crystallization of Wolinella succinogenes quinol:fumarate reductase.
Ba3-type cytochrome-c oxidase from Thermus thermophilus:
purification, crystallization, and crystal transformation.
Two-dimensional crystallization of membrane proteins: a practical guide.
In cubo crystallization of membrane proteins.