Membrane Protein Purification and Crystallization - 2nd Edition - ISBN: 9780123617767, 9780080536170

Membrane Protein Purification and Crystallization

2nd Edition

A Practical Guide

Editors: Carola Hunte Gebhard von Jagow Hermann Schagger
Paperback ISBN: 9780123617767
eBook ISBN: 9780080536170
Imprint: Academic Press
Published Date: 19th December 2002
Page Count: 316
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This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Key Features

@introbul:Key features @bul:* Provides general guidelines and strategies for isolation and crystallization of membrane proteins

  • Gives detailed protocols that have wide application, and low specialized equipment needs
  • Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins
  • Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes
  • Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization
  • Presents a comprehensive guide to 2D-crystallization of membrane proteins


Biochemists, molecular, cell, and structural biologists, and researchers in the pharmaceutical and biotechnical industry

Table of Contents

I. Strategies and Techniques

Purification Strategies for Membrane Proteins. Chromatographic Techniques and Basic Operations in Membrane Protein Purification. Production and purification of recombinant membrane proteins. SDS Electrophoresis Techniques. Blue-Native Electrophoresis. Preparative Isoelectric Focussing. Membrane protein crystallization.

II. Discussion of Selected Isolation Protocols

Lipid Dependent Inactivation and Reactivation of Bovine complex III. Purification of affinity-epitope tagged G-protein coupled receptors. Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D Crystallization. Purification of the yeast cytochrome bc1 complex.

III. Crystallization of Membrane Proteins

Antibody fragment-mediated crystallization of membrane proteins. Crystallization of Wolinella succinogenes quinol:fumarate reductase. Ba3-type cytochrome-c oxidase from Thermus thermophilus: purification, crystallization, and crystal transformation. Two-dimensional crystallization of membrane proteins: a practical guide. In cubo crystallization of membrane proteins.


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© Academic Press 2003
Academic Press
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About the Editor

Carola Hunte

Affiliations and Expertise

Max-Planck-Institut fuer Biophysik, Frankfurt, Germany

Gebhard von Jagow

Affiliations and Expertise

Zentrum Für Biologische Chemie, Frankfurt, Germany

Hermann Schagger

Affiliations and Expertise

Zentrum Für Biologische Chemie, Universitätsklinikum Frankfurt, Germany