This book provides a comprehensive overview of recent developments in the fast moving field of protein transport across and into intracellular membranes. The soluble and membrane-bound components assisting in these processes are introduced, and their functions described. Besides a detailed analysis of protein translocation across the bacterial plasma membrane, the mechanisms of protein targeting within the eukaryotic cell are discussed with special focus on the transport of proteins to the endoplasmic reticulum, vacuoles, peroxisomes, mitochondria, and chloroplasts.

Providing stimulating hypotheses and models, the book is well suited for advanced students, graduate students, and for newcomers to the field seeking a general, yet accurate introduction to present knowledge in membrane transport of proteins.

Table of Contents

Part A. Bacteria 1. Where are we in the exploration of Escherichia coli translocation pathways? (B. Wickner). 2. Components involved in bacterial protein translocation (C. Harris, P.C. Tai). 3. Molecular characterization of Sec proteins comprising the protein secretory machinery of Escherichia coli (S. Mizushima, H. Tokuda, S. Matsuyama). 4. Distinct steps in the insertion pathway of bacteriophage coat proteins (A. Kuhn, D. Troschel). 5. Steps in the assembly of a cytoplasmic membrane protein - The MalF component of the maltose transport complex (B. Traxler, J. Beckwith). 6. Structural characteristics of presecretory proteins: Their implication as to translocation competency (S. Mizushima, K. Tani, C. Hikita, M. Kato). 7. Sequence determinants of membrane protein topology (G. von Heijne). 8. Lipid involvement in protein translocation (B. de Kruijff, E. Breukink, R.A. Demel, R. van 't Hof, H.H.J. de Jongh, W. Jordi, R.C.A. Keller, J.A. Killian, A.I.P.M. de Kroon, R. Kusters, M. Pilon). Part B. Endoplasmic reticulum 9. Membrane protein insertion into the endoplasmic reticulum - signals, machinery, and mechanisms (S. High, B. Dobberstein). 10. Translocation of proteins through the endoplasmic reticulum membrane: investigation of their molecular environment by crosslinking (E. Hartmann, T.A. Rapoport). 11. The role of GTP in protein targeting to the endoplasmic reticulum (C. Ogg, J.M. Nunnari, J.D. Miller, P. Walter). 12. Consecutive steps of nucleoside triphosphate hydrolysis are driving transport of precursor proteins into the endoplasmic reticulum (P. Klappa, G. Müller, G. Schlenstedt, H. Wiech, R. Zimmermann). Part C. Vacuoles 13. Mechanisms and regulation of import and degradation of


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© 1992
Elsevier Science
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