Measurement and Manipulation of Intracellular Ions, Volume 27

1st Edition

Editor-in-Chiefs: P. Michael Conn
Serial Volume Editors: Jacob Kracier S. Dixon
Hardcover ISBN: 9780121852979
eBook ISBN: 9780080536460
Imprint: Academic Press
Published Date: 12th September 1995
Page Count: 400
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Table of Contents

Light-Optical-Based Reagents for the Measurement and Manipulation of Ions, Metabolites, and Macromolecules in Living Cells, K.A. Giuliano and D.L. Taylor. Use of Ion-Selective Microelectrodes for Recording Intracellular Ion Levels, M.E. Morris. Use of NMR Spectroscopy to Measure Intracellular Ion Concentrations, S.J. Karlik. Use of Ionophores for Manipulating Intracellular Ion Concentrations, G.A. Woolley, D.R. Pfeiffer, and C.M. Deber. Fluorescence Imaging of Cytosolic Calcium: An Introduction to Basic Experimental Principles, D.A. Williams. Fluorescence Measurements of Cytosolic Calcium: Combined Photometry with Electrophysiology, W.T. Mason, J. Dempster, R. Zorec, J. Joyland, and P.M. Lledo. Use of Chelators and Photoactivatable Caged Compounds to Manipulate Cytosolic Calcium, A.M. Gurney and S.E. Bates. Measurement of Cytosolic Calcium by 19 NMR, G.A. Smith and H.S. Bachelard. Fluorescence Measurement of Cytosolic pH in Cultured Rodent Astrocytes, S.J. Dixon and J.X. Wilson. Brain Cell Acid-Base Changes Measured with Ion-Selective Microelectrodes, C.D. Lascola and R.P. Kraig. Measurement of Cytosolic pH by Nuclear Magnetic Resonance Spectroscopy, O.A.C. Petroff and J.W. Prichard. Manipulation and Regulation of Cytosolic pH, M.O. Bevensee and W.F. Boron. Fluorescence Measurements of Cytosolic Sodium Concentration, M.A. Robertson and J.K. Foskett. Measurement of Cytosolic Sodium Using Ion-Selective Microelectrodes, J.W. Deitmer and T. Munsch. A Practical Guide to the Use of Fluorescent Indicators for the Measurement of Cytosolic Free Magnesium, E. Murphy and R.E. London. Measurement of Cytosolic Magnesium by 31P NMR Sp


Description

This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.

Key Features

@introbul:Key Features @bul:* A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies

  • Includes overviews of @subbul:* Optical probes and reagents
  • Fabrication and use of ion-selective microelectrodes
  • Use of NMR spectroscopy
  • Ionophores

Details

No. of pages:
400
Language:
English
Copyright:
© Academic Press 1995
Published:
Imprint:
Academic Press
eBook ISBN:
9780080536460
Hardcover ISBN:
9780121852979

About the Editor-in-Chiefs

P. Michael Conn Editor-in-Chief

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA

About the Serial Volume Editors

Jacob Kracier Serial Volume Editor

Affiliations and Expertise

The University of Western Ontario, Health Sciences Centre, London, Canada and Human Frontier Science Program, Strasbourg, France

S. Dixon Serial Volume Editor

Affiliations and Expertise

The University of Western Ontario, Health Sciences Centre, London, Canada