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Mass Spectrometry - 1st Edition - ISBN: 9780121820947, 9780080881140

Mass Spectrometry, Volume 193

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: James McCloskey
eBook ISBN: 9780080881140
Imprint: Academic Press
Published Date: 28th December 1990
Page Count: 960


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Table of Contents

J.A. McCloskey, Introduction. General Techniques: R.J. Cotter and A.G. Harrison, Methods of Ionization. K.R. Jennings and G.G. Dolnikowski, Mass Analyzers. S. Evans, Detectors. J.T. Watson, Selected Ion Measurements. M.L. Vestal, Liquid Chromatography-Mass Spectrometry. M.L. Gross, Tandem Mass Spectrometry: Multisector Magnetic Instruments. R.A. Yost and R.K. Boyd, Tandem Mass Spectrometry: Quadrupole and Hybrid Instruments. E. De Pauw, Matrix Selection for Liquid Secondary Ion and Fast Atom Bombardment Mass Spectrometry. R.M. Caprioli, Continuous-Flow Fast Atom Bombardment Mass Spectrometry. M.L. Gross and R.N. Hayes, Collison-Induced Dissociation. R.D. Macfarlane, Principles of Californium-252 Plasma Desorption Mass Spectrometry Applied to Protein Analysis. F. Hillenkamp and M. Karas, Mass Spectrometry of Peptides and Proteins by Matrix-Assisted Ultraviolet Laser Desorption/Ionization. K. Biemann, Utility of Exact Mass Measurements. R.M. Milberg, Selection and Use of Mass Reference Standards. D.R. Knapp, Chemical Derivatization for Mass Spectrometry. J.A. McCloskey, Introduction of Deuterium by Exchange for Measurement by Mass Spectrometry. R.C. Murphy and K.L. Clay, Preparation of Labeled Molecules by Exchange with Oxygen-18 Water. Peptides and Proteins: K. Biemann, Peptides and Proteins: Overview and Strategy. T.D. Lee and J.E. Shively, Enzymatic and Chemical Digestion of Proteins for Mass Spectrometry. D.L. Smith and Z. Zhou, Strategies for Locating Disulfide Bonds in Proteins. K.L. Stone, J.I. Elliott, G. Peterson, W. McMurray, and K.R. Williams, Reversed-Phase High-Performance Liquid Chromatography for Fractionation of Enzymatic Digests and Chemical Cleavage Products of Proteins. C.G. Edmonds and R.D. Smith, Electrospray Ionization Mass Spectrometry. P. Roepstorff, Sample Preparation for Plasma Desorption Mass Spectrometry. I. Jardine, Molecular Weight Analysis of Proteins. K. Biemann, Sequencing of Peptides by Tandem Mass Spectrometry on High-Energy Collision-Induced Dissocation. B.W. Gibson and P. Cohen, Liquid Secondary Ion Mass Spectrometry of Phosphorylated and Sulfated Peptides and Proteins. S.A. Carr, J.R. Barr, G.D. Roberts, K. Anumula, and P.B. Taylor, Identification of Attachment Sites and Structural Classes of Asparagine-Linked Carbohydrates in Glycoproteins. H.A. Scoble and S.A. Martin, Characterization of Recombinant Proteins. Glycoconjugates: R.A. Laine, Glycoconjugates: Overview and Strategy. C.G. Hellerqvist, Linkage Analysis Using Lindberg Method. G.R. Gray, Linkage Analysis Using Reductive Cleavage Method. A.-S. Angel and B. Nilsson, Linkage Positions in Glycoconjugates by Periodate Oxidation and Fast Atom Bombardment Mass Spectrometry. D.R. Maduller, B. Domon, and W.J. Richter, Tandem Mass Spectrometry in Structural Characterization of Oligosaccharide Residues in Glycoconjugates. B.E. Samuelsson, W. Pimlott, and K.-A. Karlsson, Mass Spectrometry of Mixtures of Intact Glycosphingolipids. A. Dell, Preparation and Desorption Mass Spectrometry of Permethyl and Peracetyl Derivatives of Oligosaccharides. L. Poulter and A.L. Burlingame, Desorption Mass Spectrometry of Oligosaccharides Coupled with Hydrophobic Chromophores. A.L. Burlingame and B.L. Gillece-Castro, Oligosaccharide Characterization with High-Energy Collision-Induced Dissociation Mass Spectrometry. J. Peter-Kataliniaac and H. Egge, Desorption Mass Spectrometry of Glycosphingolipids. G.C. Hansson and H. Karlsson, High-Mass Gas Chromatography-Mass Spectrometry. C.E. Costello and J.E. Vath, Tandem Mass Spectrometry of Glycolipids. Nucleotides and Nucleosides: J.A. McCloskey, Constituents of Nucleic Acids: Overview and Strategy. P.F. Crain, Preparation and Enzymatic Digestion of DNA and RNA for Mass Spectrometry. K.H. Schram, Preparation of Trimethylsilyl Derivatives of Nucleic Acid Components for Analysis by Mass Spectrometry. S.C. Pomerantz and J.A. McCloskey, Analysis of RNA Hydrolyzates by Liquid Chromatography-Mass Spectrometry. J.A. McCloskey, Electron Ionization Mass Spectra of Trimethylsilyl Derivatives of Nucleosides. M. Dizdaroglu, Gass Chromatography-Mass Spectrometry of Free Radical-Induced Products of Pyrimidines and Purines in DNA. P.F. Crain, Analysis of 5-Methylcytosine in DNA by Isotope Dilution Gas Chromatography-Mass Spectrometry. Appendixes: Isotopic Exact Masses and Abundances. Reference Ions for Exact Mass Measurements. Matrix Cluster Ions. Calculation of Isotopic Abundance Patterns. Nomenclature for Peptide Fragment Ions. Residue Mass for Peptides. Author Index. Subject Index.


This volume presents a compilation of experimental methods and principles, with detailed protocols and examples. Techniques which have demonstrated value in problem-solving are included. Intended for biochemists, cell biologists, microbiologists, pharmacologists, biomedical scientists, biophysicists, analytical chemists, and physiologists, this book focuses on mass spectrometry. Topics discussed include the methods of ionization, liquid chromatography, continuous flow fast atom bombardment mass spectrometry, collision-induced dissociation, enzymatic and chemical digestion of proteins, and constituents of nucleic acids. Other titles in this series include Guide to Yeast Genetics and Molecular Biology and Retinoids, Part B: Cell Differentiation and Clinical Applications.


Biochemists, analytical and organic chemists, biophysicists, molecular biologists, and physical and pharmaceutical chemists.


No. of pages:
© Academic Press 1990
28th December 1990
Academic Press
eBook ISBN:


@from:Praise for the Volume @qu:"This volume will be a most welcome addition to the shelves of those working with mass spectrometry (MS) applications... The book is useful and comprehensive, offering a concise treatment of experimental principles along with detailed descriptions of specific methods... This book is a gold mine of information. The standards of excellence established in the Methods in Enzymology series have been continued in this volume. The 70 authors and the editor are to be congratulated." @source:--SPECTROSCOPY @qu:"The Methods in Enzymology series has a well-established reputation for producing carefully edited, authoritative, practical summaries of current research by experts in various specialty fields. J.A. McCloskey has continued that high standard in his selection of 70 scientists who have written 47 chapters... occupies a niche not filled previously by any single publication in the field... There are six very useful appendices to help guarantee that this volume will find a permanent home close to the operator of mass spectrometer (elemental mass and abundance data, ions in 11 defined reference materials for different types of ionization, characteristic spectra of seven FAB matrices, calculation of isotopic abundance distributions, nomenclature for peptide fragment ions, and residue masses for peptides). In summary, this book is an extremely valuable resource for the practicing mass spectroscopist. Due to the inclusion of general introductory material, it would be an excellent text for a course in biological mass spectrometry directed toward graduate students in chemistry or biochemistry." @source:--BOOK REVIEWS @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

James McCloskey

Affiliations and Expertise

University of Utah, Salt Lake City, U.S.A.