This volume provides comprehensive treatment of tools and proper usage for the identification of proteins, affinity chromatography and studies the complexity of protein machines and assemblages, assignment of the most common protein posttranslational modifications (phosphorylation and glycosylation) and glycolipidomics.

Key Features

*Part 2 of 2 volumes about Mass Spectrometry *Discusses peptide and protein cleanup and preparation requirements for mass spectrometry *Explains protein enzymic and chemical digestion strategies *Includes case studies of protein assemblages and machines


Biochmemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists

Table of Contents

Chapter 1 Molecular Weight Determination of Peptides and proteins by ESI and MALDI ; Chapter 2 Batch Introduction Techniques ; Chapter 3 In-solution digestion of proteins for mass spectrometry ; Chapter 4 Identification of phosphorylation sites using micro immobilised metal affinity chromatography ; Chapter 5 Mapping Posttransitional Modifications of Proteins by MS-based selective Detection : Application to Phosphoproteins ; Chapter 6 Characterization of protein N-glycosylation ; Chapter 7 O-Glycosylation of Proteins ;Chapter 8 Analysis of Glycosylphosphatidylinositol (GPI) Protein Ancors – The Prion Protein ; Chapter 9 Comprehensive mass spectrometric analysis of the proteasome complex; Chapter 10 The Analysis of Multiprotein Complexes: The Yeast and Human Spliceosome as Case Studies; Chapter 11 The Use of Mass Spectrometry in the Identification of Antigens from Proteasome processing; Chapter 12 Glycosphingolipid Structural Analysis and Glycosphingolipidomics; Chapter 13 Mapping Bacterial Glycolipid Complexity Using Capillary Electrophoresis and Electrospray Mass Spectrometry


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© 2005
Academic Press
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